Edaphobacter aggregans

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Edaphobacter

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A428MMJ2|A0A428MMJ2_9BACT 30S ribosomal protein S12 OS=Edaphobacter aggregans OX=570835 GN=rpsL PE=3 SV=1
MM1 pKa = 7.57AAQAPSRR8 pKa = 11.84WILLALLGLAGTLTTQTAMADD29 pKa = 3.29SFTFAFSGGGISASGIITVSPTATPGTDD57 pKa = 3.21TITGISGFFSDD68 pKa = 4.64TNASANFSGAITGLEE83 pKa = 4.07FAPPPSSPPPFPAPAFTASGFSYY106 pKa = 11.4DD107 pKa = 3.45NLFYY111 pKa = 10.75TDD113 pKa = 3.73GNSPLVCPPEE123 pKa = 4.5TPGGPPGYY131 pKa = 9.1PFAGGFLDD139 pKa = 3.51IYY141 pKa = 11.2GVAFDD146 pKa = 3.74VAGGYY151 pKa = 7.7TVDD154 pKa = 3.11LWSNGVVPEE163 pKa = 4.5AGLTYY168 pKa = 10.12EE169 pKa = 4.2VSDD172 pKa = 4.14AFGTTLLEE180 pKa = 4.61PDD182 pKa = 4.09NEE184 pKa = 4.41GQAVPVSLSTSPIPEE199 pKa = 4.88PGSLLLMGTGLFGLVDD215 pKa = 3.24VLRR218 pKa = 11.84RR219 pKa = 11.84RR220 pKa = 11.84SKK222 pKa = 10.75HH223 pKa = 5.71SLGFDD228 pKa = 3.08AA229 pKa = 6.42

Molecular weight: 23.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R9PUH0|A0A3R9PUH0_9BACT Uncharacterized protein DUF4231 OS=Edaphobacter aggregans OX=570835 GN=EDE15_3704 PE=4 SV=1
MM1 pKa = 7.51PAPRR5 pKa = 11.84RR6 pKa = 11.84LLLLPPHH13 pKa = 6.94HH14 pKa = 7.29PPPSPGPAQPPSPHH28 pKa = 6.56RR29 pKa = 11.84PLRR32 pKa = 11.84PPHH35 pKa = 5.97ARR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 5.64PTLHH46 pKa = 6.39RR47 pKa = 11.84RR48 pKa = 11.84NPPPHH53 pKa = 6.92RR54 pKa = 11.84PQRR57 pKa = 11.84DD58 pKa = 3.25RR59 pKa = 11.84PPPRR63 pKa = 11.84RR64 pKa = 11.84PPPLRR69 pKa = 11.84PANRR73 pKa = 11.84QHH75 pKa = 6.31QPTPRR80 pKa = 11.84PQTHH84 pKa = 6.77PQHH87 pKa = 6.28QSPLRR92 pKa = 11.84TPSHH96 pKa = 5.5LHH98 pKa = 5.9RR99 pKa = 11.84RR100 pKa = 11.84HH101 pKa = 5.25HH102 pKa = 5.88HH103 pKa = 6.27RR104 pKa = 11.84PRR106 pKa = 11.84ARR108 pKa = 11.84PPRR111 pKa = 11.84SSIRR115 pKa = 11.84VRR117 pKa = 11.84PGHH120 pKa = 6.21RR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84PPQEE127 pKa = 3.42HH128 pKa = 6.82HH129 pKa = 7.08APTPRR134 pKa = 11.84PVRR137 pKa = 11.84PRR139 pKa = 11.84PTHH142 pKa = 6.3PRR144 pKa = 11.84TQTRR148 pKa = 11.84NPPHH152 pKa = 6.8PPSHH156 pKa = 6.28RR157 pKa = 11.84RR158 pKa = 11.84KK159 pKa = 7.58PTNIGVIRR167 pKa = 11.84ANRR170 pKa = 11.84CC171 pKa = 2.93

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4941

0

4941

1776201

29

6628

359.5

39.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.569 ± 0.047

0.861 ± 0.011

5.038 ± 0.028

5.138 ± 0.045

3.971 ± 0.028

7.981 ± 0.04

2.211 ± 0.018

5.191 ± 0.027

3.707 ± 0.032

9.815 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.019

3.639 ± 0.041

5.461 ± 0.028

3.888 ± 0.026

5.885 ± 0.048

6.508 ± 0.036

6.527 ± 0.073

7.242 ± 0.035

1.384 ± 0.017

2.769 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski