Klebsiella phage N1M2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Petsuvirus; unclassified Petsuvirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B7ZEI9|A0A6B7ZEI9_9CAUD Putative SbcD OS=Klebsiella phage N1M2 OX=2664939 GN=N1M2_44 PE=4 SV=1
MM1 pKa = 7.12LQSTNAAGITEE12 pKa = 4.01NMVTYY17 pKa = 10.28KK18 pKa = 10.74FKK20 pKa = 11.4VPDD23 pKa = 3.71LADD26 pKa = 3.63LFEE29 pKa = 4.95TFTPDD34 pKa = 2.81QSHH37 pKa = 6.71IALEE41 pKa = 4.31GALNIIFGRR50 pKa = 11.84APAQLAAVKK59 pKa = 10.79LEE61 pKa = 4.11GEE63 pKa = 4.2EE64 pKa = 4.44AEE66 pKa = 4.3PEE68 pKa = 4.06QTEE71 pKa = 4.3EE72 pKa = 4.43EE73 pKa = 4.27LQQDD77 pKa = 4.04HH78 pKa = 5.86EE79 pKa = 4.51TQIVGEE85 pKa = 4.2VAYY88 pKa = 10.35ALYY91 pKa = 9.08CHH93 pKa = 6.52QMGAEE98 pKa = 3.96AAALVPEE105 pKa = 5.31GCATAWTIPDD115 pKa = 3.97DD116 pKa = 4.18YY117 pKa = 11.06EE118 pKa = 5.13NSEE121 pKa = 3.86QYY123 pKa = 11.01QRR125 pKa = 11.84MEE127 pKa = 3.68EE128 pKa = 4.03MFRR131 pKa = 11.84GIVSHH136 pKa = 6.9ISNHH140 pKa = 4.83IAADD144 pKa = 3.64KK145 pKa = 10.61FNQDD149 pKa = 3.06VITLHH154 pKa = 8.0GEE156 pKa = 3.75MEE158 pKa = 4.3FFSRR162 pKa = 11.84IEE164 pKa = 4.12LYY166 pKa = 9.88TYY168 pKa = 10.85NYY170 pKa = 8.47NTDD173 pKa = 3.86CEE175 pKa = 4.49VLSVTIAAMLSS186 pKa = 3.49

Molecular weight: 20.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B7ZFB7|A0A6B7ZFB7_9CAUD Uncharacterized protein OS=Klebsiella phage N1M2 OX=2664939 GN=N1M2_229 PE=4 SV=1
MM1 pKa = 7.52LLSLLEE7 pKa = 4.57RR8 pKa = 11.84IKK10 pKa = 10.93GWVKK14 pKa = 8.47PTPKK18 pKa = 10.12QPTIEE23 pKa = 4.26FVSLTSISTTEE34 pKa = 3.65CMGWVKK40 pKa = 10.49VYY42 pKa = 10.66HH43 pKa = 6.37NKK45 pKa = 10.2LVIRR49 pKa = 11.84VNGVTRR55 pKa = 11.84IKK57 pKa = 9.8RR58 pKa = 11.84TKK60 pKa = 9.7TNSQLTCHH68 pKa = 6.35SFRR71 pKa = 11.84RR72 pKa = 11.84MFEE75 pKa = 3.77MCIGTGEE82 pKa = 4.22TLHH85 pKa = 6.81IARR88 pKa = 11.84SKK90 pKa = 10.61YY91 pKa = 10.54KK92 pKa = 9.98IVLSAKK98 pKa = 9.59FKK100 pKa = 11.24AGIGKK105 pKa = 8.84NFII108 pKa = 4.14

Molecular weight: 12.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

257

0

257

78103

29

3375

303.9

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.082 ± 0.167

0.853 ± 0.051

6.517 ± 0.097

6.477 ± 0.167

4.392 ± 0.114

6.605 ± 0.167

1.777 ± 0.089

7.078 ± 0.135

6.827 ± 0.123

8.149 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.097

5.808 ± 0.124

4.105 ± 0.136

3.367 ± 0.083

4.49 ± 0.119

5.958 ± 0.113

6.443 ± 0.187

6.929 ± 0.155

1.218 ± 0.067

4.11 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski