Ralstonia phage RSY1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Aresaunavirus; Ralstonia virus RSY1

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077K9Y4|A0A077K9Y4_9CAUD Uncharacterized protein OS=Ralstonia phage RSY1 OX=1530085 PE=3 SV=1
MM1 pKa = 7.26ATIDD5 pKa = 4.53LSQLPAPAVVEE16 pKa = 4.17TLDD19 pKa = 3.58YY20 pKa = 10.97EE21 pKa = 5.09AILAEE26 pKa = 4.14RR27 pKa = 11.84KK28 pKa = 10.13DD29 pKa = 4.24YY30 pKa = 10.68FVSLYY35 pKa = 10.33PADD38 pKa = 3.38QRR40 pKa = 11.84DD41 pKa = 3.64AVRR44 pKa = 11.84ATLEE48 pKa = 4.38LEE50 pKa = 4.22SEE52 pKa = 4.97PITKK56 pKa = 10.36LLQEE60 pKa = 3.83NAYY63 pKa = 10.37RR64 pKa = 11.84EE65 pKa = 4.4LVWRR69 pKa = 11.84QRR71 pKa = 11.84VNDD74 pKa = 3.86AARR77 pKa = 11.84GVMLAFAEE85 pKa = 4.78GEE87 pKa = 4.05DD88 pKa = 4.37LEE90 pKa = 5.07QIAANFNVQRR100 pKa = 11.84LTITPADD107 pKa = 4.13DD108 pKa = 3.49TTVPPTPAVMEE119 pKa = 4.7GDD121 pKa = 3.37DD122 pKa = 4.08SLRR125 pKa = 11.84EE126 pKa = 3.81RR127 pKa = 11.84AQEE130 pKa = 3.88AFEE133 pKa = 4.36GLSVAGPTKK142 pKa = 10.29AYY144 pKa = 9.83EE145 pKa = 4.0QFARR149 pKa = 11.84SADD152 pKa = 3.31GRR154 pKa = 11.84VADD157 pKa = 4.86ARR159 pKa = 11.84AISPAGAEE167 pKa = 4.17VVVSVLSHH175 pKa = 6.7LNDD178 pKa = 3.36GTADD182 pKa = 3.56EE183 pKa = 4.84SLLATVRR190 pKa = 11.84TALSDD195 pKa = 3.81DD196 pKa = 3.66DD197 pKa = 4.2TRR199 pKa = 11.84PLGDD203 pKa = 3.4RR204 pKa = 11.84LTVQSATIVPYY215 pKa = 10.48RR216 pKa = 11.84IRR218 pKa = 11.84ATLYY222 pKa = 10.2LASGPAAEE230 pKa = 5.8PILDD234 pKa = 3.94AAGKK238 pKa = 10.13RR239 pKa = 11.84ADD241 pKa = 3.48TYY243 pKa = 8.62RR244 pKa = 11.84TTRR247 pKa = 11.84RR248 pKa = 11.84RR249 pKa = 11.84IGRR252 pKa = 11.84DD253 pKa = 2.83INRR256 pKa = 11.84SAITAALHH264 pKa = 5.37VEE266 pKa = 4.34GVEE269 pKa = 3.92KK270 pKa = 10.8LVLHH274 pKa = 6.68EE275 pKa = 4.3PAEE278 pKa = 4.85DD279 pKa = 3.24IALDD283 pKa = 3.93LTQAGYY289 pKa = 8.4CTGVDD294 pKa = 3.11IVNGGTSEE302 pKa = 3.94

Molecular weight: 32.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077K9Q8|A0A077K9Q8_9CAUD Uncharacterized protein OS=Ralstonia phage RSY1 OX=1530085 PE=3 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84CPCLYY8 pKa = 9.37PVPGTRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84LSLAAQAGEE25 pKa = 4.32APTGAAYY32 pKa = 8.86WCICCEE38 pKa = 4.95LLTKK42 pKa = 9.19WAPRR46 pKa = 11.84GSALQIGTKK55 pKa = 10.07VPLRR59 pKa = 11.84GFSRR63 pKa = 11.84MVKK66 pKa = 10.07LRR68 pKa = 11.84ADD70 pKa = 3.5NKK72 pKa = 10.22EE73 pKa = 3.84GEE75 pKa = 4.16FPIIRR80 pKa = 11.84KK81 pKa = 8.67VFNLSPGLIFRR92 pKa = 11.84LLNIAHH98 pKa = 7.26

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12425

55

901

253.6

27.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.797 ± 0.521

0.934 ± 0.129

5.795 ± 0.197

5.416 ± 0.231

3.219 ± 0.193

7.831 ± 0.39

2.254 ± 0.26

3.903 ± 0.169

3.855 ± 0.258

8.869 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.136

3.292 ± 0.199

4.909 ± 0.219

4.209 ± 0.216

7.863 ± 0.437

5.078 ± 0.219

5.755 ± 0.376

7.509 ± 0.252

1.65 ± 0.13

2.704 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski