Candidatus Magnetoglobus multicellularis str. Araruama

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Candidatus Magnetoglobus; Candidatus Magnetoglobus multicellularis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1NSZ5|A0A1V1NSZ5_9DELT Glutamine--scyllo-inositol transaminase (Fragment) OS=Candidatus Magnetoglobus multicellularis str. Araruama OX=890399 GN=OMM_13869 PE=4 SV=1
AA1 pKa = 7.55FADD4 pKa = 3.82YY5 pKa = 11.36DD6 pKa = 3.86NDD8 pKa = 3.76GDD10 pKa = 4.82LDD12 pKa = 3.74ILITGDD18 pKa = 3.38SDD20 pKa = 3.33NGKK23 pKa = 8.01IAKK26 pKa = 8.11MFRR29 pKa = 11.84NTGNAFTEE37 pKa = 4.1DD38 pKa = 2.96TGINLTGVSHH48 pKa = 7.13SSTAFGDD55 pKa = 3.58YY56 pKa = 11.14DD57 pKa = 4.16NDD59 pKa = 3.76GDD61 pKa = 5.21LDD63 pKa = 4.15LLITGRR69 pKa = 11.84SDD71 pKa = 3.34SLIIAKK77 pKa = 9.17MYY79 pKa = 10.75QNTGGNFSEE88 pKa = 4.55DD89 pKa = 3.16TTINLMGVEE98 pKa = 4.26YY99 pKa = 10.65GSTSFGDD106 pKa = 3.56YY107 pKa = 11.22DD108 pKa = 4.13NDD110 pKa = 3.69GDD112 pKa = 5.0LDD114 pKa = 3.82ILISGDD120 pKa = 3.15TGSNKK125 pKa = 7.64ITKK128 pKa = 9.65VYY130 pKa = 10.36RR131 pKa = 11.84NTGGSFSEE139 pKa = 4.27DD140 pKa = 2.97TGIHH144 pKa = 6.76LIGVFDD150 pKa = 4.03GASAFGDD157 pKa = 3.7YY158 pKa = 11.26DD159 pKa = 3.87NDD161 pKa = 3.71GDD163 pKa = 5.07LDD165 pKa = 3.76ILITGDD171 pKa = 3.21TGNSKK176 pKa = 8.52NTTVYY181 pKa = 10.9QNTDD185 pKa = 2.67GHH187 pKa = 6.37FSEE190 pKa = 6.03ASDD193 pKa = 4.03INLTDD198 pKa = 3.55VTHH201 pKa = 5.83STTVFGDD208 pKa = 3.33YY209 pKa = 11.18DD210 pKa = 4.0NDD212 pKa = 3.83GDD214 pKa = 5.59LDD216 pKa = 3.75ILMSGQAASGRR227 pKa = 11.84MAKK230 pKa = 9.8IYY232 pKa = 10.51QNNTLISNTPANAPSSLTSVVSEE255 pKa = 4.2QIVLLSWSAASDD267 pKa = 4.01SEE269 pKa = 4.45TLSAAGLSYY278 pKa = 11.08NLRR281 pKa = 11.84IGSSPGACDD290 pKa = 3.09ILSPMALPLSNGYY303 pKa = 8.95RR304 pKa = 11.84QIPARR309 pKa = 11.84GAIQTLTATINPLNGGTYY327 pKa = 7.97YY328 pKa = 10.33WSVQSIDD335 pKa = 3.39TAFAGSDD342 pKa = 3.41FSNEE346 pKa = 3.77STFTIDD352 pKa = 2.97QTPIISAIAHH362 pKa = 4.54QHH364 pKa = 4.41TVIDD368 pKa = 4.14YY369 pKa = 7.81PISIPFQLL377 pKa = 4.32

Molecular weight: 39.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V1NVQ3|A0A1V1NVQ3_9DELT Uncharacterized protein (Fragment) OS=Candidatus Magnetoglobus multicellularis str. Araruama OX=890399 GN=OMM_12535 PE=4 SV=1
MM1 pKa = 7.81ASFEE5 pKa = 4.65KK6 pKa = 10.49PLEE9 pKa = 4.19TRR11 pKa = 11.84FLRR14 pKa = 11.84PSQRR18 pKa = 11.84KK19 pKa = 7.17LTASFTLSKK28 pKa = 10.41CLYY31 pKa = 8.92SWYY34 pKa = 10.63SPAARR39 pKa = 11.84AATPMIVAPTGLAARR54 pKa = 11.84VMRR57 pKa = 11.84DD58 pKa = 3.35VPTMPIAPLSMPRR71 pKa = 11.84PDD73 pKa = 3.69VSAANNGPAVARR85 pKa = 11.84PAAITPYY92 pKa = 9.88AAPAAPITATTVPAAAATPVSPIAIFVSMGCAVMKK127 pKa = 9.81PATLFAIFKK136 pKa = 10.19IIVPTGARR144 pKa = 11.84ATATLAADD152 pKa = 4.61LMNSLLTVRR161 pKa = 11.84HH162 pKa = 6.33ASPSSRR168 pKa = 11.84VRR170 pKa = 11.84VLDD173 pKa = 3.56VSNCRR178 pKa = 11.84FNVPEE183 pKa = 4.46TFPSTLSGDD192 pKa = 3.46

Molecular weight: 20.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9976

0

9976

3167227

32

7151

317.5

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.941 ± 0.027

1.199 ± 0.009

6.279 ± 0.02

5.698 ± 0.024

4.495 ± 0.019

5.95 ± 0.029

2.249 ± 0.012

8.781 ± 0.022

6.399 ± 0.04

8.762 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.015

5.95 ± 0.023

3.825 ± 0.018

4.303 ± 0.018

3.783 ± 0.024

7.336 ± 0.037

5.983 ± 0.043

5.568 ± 0.021

1.164 ± 0.01

3.888 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski