Southern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus; Southern bean mosaic virus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O73571|MVP_SBMVA Movement protein P1 OS=Southern bean mosaic virus (isolate Bean/United States/Arkansas) OX=652938 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.91SYY3 pKa = 10.9RR4 pKa = 11.84FLTVRR9 pKa = 11.84AFGFTGFHH17 pKa = 6.97CDD19 pKa = 2.89ATRR22 pKa = 11.84LLSEE26 pKa = 4.57TEE28 pKa = 4.32VIDD31 pKa = 4.1VPSSLDD37 pKa = 3.5FVGEE41 pKa = 4.15TEE43 pKa = 5.27LRR45 pKa = 11.84LEE47 pKa = 4.47TAWPQCEE54 pKa = 4.19EE55 pKa = 3.82NCYY58 pKa = 10.2TILPRR63 pKa = 11.84FNVQVDD69 pKa = 3.94FEE71 pKa = 4.84YY72 pKa = 11.17YY73 pKa = 8.29PVRR76 pKa = 11.84VEE78 pKa = 3.87IVCRR82 pKa = 11.84VCAASLSVIFSKK94 pKa = 10.5WDD96 pKa = 4.2FYY98 pKa = 11.06CSRR101 pKa = 11.84RR102 pKa = 11.84GHH104 pKa = 6.15FVPVDD109 pKa = 3.46QNGDD113 pKa = 3.49LFRR116 pKa = 11.84IGTLQEE122 pKa = 3.75TGEE125 pKa = 4.33KK126 pKa = 10.56YY127 pKa = 10.47FYY129 pKa = 10.88FCDD132 pKa = 3.9KK133 pKa = 10.67SICRR137 pKa = 11.84QCIIQAAHH145 pKa = 5.75HH146 pKa = 6.36HH147 pKa = 5.58SS148 pKa = 3.48

Molecular weight: 17.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O73564|P2A_SBMVA Polyprotein P2A OS=Southern bean mosaic virus (isolate Bean/United States/Arkansas) OX=652938 GN=ORF2A PE=4 SV=2
MM1 pKa = 7.87AKK3 pKa = 10.33RR4 pKa = 11.84LTKK7 pKa = 10.27QQLTKK12 pKa = 10.82AIANTLEE19 pKa = 4.14APATQSRR26 pKa = 11.84RR27 pKa = 11.84PRR29 pKa = 11.84NRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SAARR39 pKa = 11.84QPQSTQAGVSMAPIAQGTMVRR60 pKa = 11.84LRR62 pKa = 11.84EE63 pKa = 4.0PSLRR67 pKa = 11.84TAGGVTVLTHH77 pKa = 6.43SEE79 pKa = 4.11LSTEE83 pKa = 4.12LSVTNAIVITSEE95 pKa = 4.02LVMPYY100 pKa = 10.63TMGTWLRR107 pKa = 11.84GVAANWSKK115 pKa = 11.51YY116 pKa = 9.42SLLSVRR122 pKa = 11.84YY123 pKa = 7.52TYY125 pKa = 11.0LPSCPSTTSGSIHH138 pKa = 6.61MGFQYY143 pKa = 11.56DD144 pKa = 3.61MADD147 pKa = 3.6TLPVSVNQLSNLRR160 pKa = 11.84GYY162 pKa = 11.09VSGQVWSGSSGLCYY176 pKa = 10.31INGTRR181 pKa = 11.84CLDD184 pKa = 3.41TANAITTTLDD194 pKa = 2.86VGQLGKK200 pKa = 10.22KK201 pKa = 8.7WYY203 pKa = 9.03PFKK206 pKa = 10.71TSTDD210 pKa = 3.44FTTAVGVNVNIATPLVPARR229 pKa = 11.84LIIAMLDD236 pKa = 3.78GSSSTAVSTGRR247 pKa = 11.84LYY249 pKa = 11.22VSYY252 pKa = 9.44TIQLIEE258 pKa = 4.11PTALALNNN266 pKa = 3.79

Molecular weight: 28.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1951

148

962

487.8

53.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.176 ± 0.516

2.665 ± 0.503

4.562 ± 0.64

5.843 ± 0.931

3.69 ± 0.754

7.227 ± 0.437

1.896 ± 0.361

4.1 ± 0.266

5.126 ± 0.948

9.124 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.794 ± 0.301

2.87 ± 0.403

6.458 ± 0.913

3.537 ± 0.362

5.382 ± 0.684

9.995 ± 0.579

5.792 ± 1.717

7.381 ± 0.206

2.101 ± 0.268

3.28 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski