Shuangao Bedbug Virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA negative-strand viruses

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXF4|A0A0B5KXF4_9VIRU Glycoprotein OS=Shuangao Bedbug Virus 2 OX=1608072 GN=G PE=4 SV=1
MM1 pKa = 7.29CQFPNMLMFILILKK15 pKa = 10.2SGVTGQHH22 pKa = 6.47PKK24 pKa = 10.77LSFISHH30 pKa = 6.95LDD32 pKa = 2.81KK33 pKa = 11.78GEE35 pKa = 3.84AVKK38 pKa = 10.59INPIPQCKK46 pKa = 8.74TDD48 pKa = 3.08WSKK51 pKa = 8.04TTVYY55 pKa = 10.8LPGTLMVLRR64 pKa = 11.84PNPKK68 pKa = 9.04LLKK71 pKa = 10.61SIGLLATAFTVKK83 pKa = 9.75TKK85 pKa = 10.68CEE87 pKa = 3.8KK88 pKa = 10.6FLFSQNVRR96 pKa = 11.84TTIYY100 pKa = 10.37DD101 pKa = 3.54SPIPWSSFDD110 pKa = 3.62DD111 pKa = 3.61SFIRR115 pKa = 11.84FFEE118 pKa = 4.13EE119 pKa = 5.4KK120 pKa = 8.68YY121 pKa = 8.81TKK123 pKa = 9.99PVQLEE128 pKa = 3.93KK129 pKa = 11.04LQLKK133 pKa = 10.45VSGEE137 pKa = 3.95EE138 pKa = 4.95LMGQDD143 pKa = 5.19LYY145 pKa = 11.93SCGYY149 pKa = 10.49YY150 pKa = 10.17SGQFQNINYY159 pKa = 9.1IKK161 pKa = 9.93FKK163 pKa = 10.7AVEE166 pKa = 4.4TEE168 pKa = 3.43WDD170 pKa = 3.72FQGNLLSPIFDD181 pKa = 4.07PSCLHH186 pKa = 5.85NHH188 pKa = 5.96TSCVLKK194 pKa = 10.45EE195 pKa = 3.88DD196 pKa = 3.76SKK198 pKa = 11.8LYY200 pKa = 10.83FPMGSPNLGHH210 pKa = 6.88CEE212 pKa = 3.59FSTISTLAGFFLSQSSKK229 pKa = 8.95ISSFVSHH236 pKa = 6.62NSAFKK241 pKa = 10.29TYY243 pKa = 10.56FDD245 pKa = 3.74WSLKK249 pKa = 9.52GSHH252 pKa = 6.7PRR254 pKa = 11.84SLCHH258 pKa = 6.57YY259 pKa = 8.53ITGNHH264 pKa = 6.41YY265 pKa = 8.74ITLEE269 pKa = 4.04GHH271 pKa = 5.37VVRR274 pKa = 11.84ALDD277 pKa = 3.74SEE279 pKa = 4.24GRR281 pKa = 11.84DD282 pKa = 3.23IFIKK286 pKa = 10.39SQPNPLFSQPGNHH299 pKa = 6.56RR300 pKa = 11.84IKK302 pKa = 10.58RR303 pKa = 11.84SEE305 pKa = 4.18HH306 pKa = 6.35LPLTTINTTLSQLEE320 pKa = 4.41FPKK323 pKa = 10.6FPPPSLKK330 pKa = 10.13FRR332 pKa = 11.84TSQSVSRR339 pKa = 11.84SKK341 pKa = 10.78RR342 pKa = 11.84SYY344 pKa = 10.9NYY346 pKa = 8.66YY347 pKa = 10.45NYY349 pKa = 9.29PARR352 pKa = 11.84QRR354 pKa = 11.84EE355 pKa = 4.03QMLYY359 pKa = 9.89EE360 pKa = 4.17HH361 pKa = 6.95SSWTLSEE368 pKa = 5.1LEE370 pKa = 4.37FEE372 pKa = 5.65LSLLHH377 pKa = 6.39NEE379 pKa = 4.82SIAEE383 pKa = 3.98IQKK386 pKa = 10.34RR387 pKa = 11.84WILEE391 pKa = 4.15CKK393 pKa = 9.96LKK395 pKa = 10.72NLHH398 pKa = 5.85GQLARR403 pKa = 11.84SVLHH407 pKa = 6.6IDD409 pKa = 3.08PRR411 pKa = 11.84PYY413 pKa = 9.51IAYY416 pKa = 9.52VLSSQAFITLNVNSKK431 pKa = 8.89TYY433 pKa = 10.16YY434 pKa = 9.97ISGDD438 pKa = 3.9PVWDD442 pKa = 3.29IEE444 pKa = 4.5IYY446 pKa = 10.43KK447 pKa = 9.6PYY449 pKa = 9.84KK450 pKa = 7.88WCNGSLYY457 pKa = 10.75VHH459 pKa = 6.13TEE461 pKa = 3.91KK462 pKa = 11.26GPGWLKK468 pKa = 10.91NEE470 pKa = 3.77WGIVISGDD478 pKa = 3.88LLTGGCNGSLYY489 pKa = 10.84VHH491 pKa = 6.13TEE493 pKa = 3.91KK494 pKa = 11.26GPGWLKK500 pKa = 10.91NEE502 pKa = 3.77WGIVISGDD510 pKa = 3.87LLTGGCTSNQAPEE523 pKa = 4.15IFLFPTMRR531 pKa = 11.84NGSWDD536 pKa = 4.13LISNRR541 pKa = 11.84SLSSDD546 pKa = 3.34LSLPSPHH553 pKa = 6.94HH554 pKa = 5.8LLEE557 pKa = 5.15HH558 pKa = 6.49GFYY561 pKa = 9.01KK562 pKa = 10.49TSFEE566 pKa = 4.5IDD568 pKa = 2.92TSDD571 pKa = 3.2ALLRR575 pKa = 11.84SLEE578 pKa = 4.37AGLSSMSYY586 pKa = 9.59GASSRR591 pKa = 11.84KK592 pKa = 9.2SAGVSLWKK600 pKa = 10.5IPDD603 pKa = 4.25PPSSWYY609 pKa = 10.43EE610 pKa = 3.73SMILHH615 pKa = 6.99FLTPTTMLIFGLIIFLFIWRR635 pKa = 11.84FLRR638 pKa = 11.84GG639 pKa = 3.21

Molecular weight: 72.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KXF4|A0A0B5KXF4_9VIRU Glycoprotein OS=Shuangao Bedbug Virus 2 OX=1608072 GN=G PE=4 SV=1
MM1 pKa = 7.73AEE3 pKa = 4.36SSLSTTQNHH12 pKa = 7.45KK13 pKa = 10.54ILLPKK18 pKa = 9.87NASSLTITPVEE29 pKa = 4.14QFPHH33 pKa = 8.07LDD35 pKa = 2.91IQRR38 pKa = 11.84YY39 pKa = 7.97PEE41 pKa = 4.16QKK43 pKa = 9.45SSHH46 pKa = 6.64HH47 pKa = 5.72IVIGVGNQEE56 pKa = 4.12AVWRR60 pKa = 11.84TEE62 pKa = 3.85KK63 pKa = 10.73EE64 pKa = 4.08VLSPVILHH72 pKa = 6.66LEE74 pKa = 4.32SACTVANANIPVRR87 pKa = 11.84RR88 pKa = 11.84TFKK91 pKa = 10.89LVLGLALLDD100 pKa = 3.87IYY102 pKa = 11.2HH103 pKa = 6.7NSGVSNLTIQKK114 pKa = 9.55IFSSCLEE121 pKa = 3.97IMKK124 pKa = 10.21KK125 pKa = 7.41EE126 pKa = 3.64QWRR129 pKa = 11.84IYY131 pKa = 9.09EE132 pKa = 4.51DD133 pKa = 3.1KK134 pKa = 9.92HH135 pKa = 5.69TSSVAWKK142 pKa = 9.78FLFKK146 pKa = 10.59LISVTPCIPSNVVNVKK162 pKa = 9.86NKK164 pKa = 8.52KK165 pKa = 7.27TFSGVISNGPYY176 pKa = 8.37SGLYY180 pKa = 9.5ISHH183 pKa = 6.95TFNYY187 pKa = 9.58HH188 pKa = 3.98SQEE191 pKa = 3.71YY192 pKa = 10.46DD193 pKa = 3.28LAEE196 pKa = 4.57SIYY199 pKa = 10.31HH200 pKa = 6.55RR201 pKa = 11.84SSGHH205 pKa = 5.66YY206 pKa = 9.86IIEE209 pKa = 3.98QAPNDD214 pKa = 3.95GKK216 pKa = 9.72EE217 pKa = 4.05TFIWMWDD224 pKa = 3.35QTRR227 pKa = 11.84TLPQEE232 pKa = 3.54FHH234 pKa = 6.54ARR236 pKa = 11.84LSIMEE241 pKa = 4.44HH242 pKa = 4.82YY243 pKa = 10.34KK244 pKa = 10.56KK245 pKa = 10.87YY246 pKa = 11.02NHH248 pKa = 6.75TKK250 pKa = 9.74TKK252 pKa = 10.74AITNKK257 pKa = 9.86KK258 pKa = 7.67SQEE261 pKa = 3.74YY262 pKa = 9.44DD263 pKa = 3.42APWNKK268 pKa = 10.23KK269 pKa = 6.8KK270 pKa = 9.33TKK272 pKa = 10.18

Molecular weight: 31.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3118

272

2207

1039.3

119.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.849 ± 0.416

1.86 ± 0.196

3.784 ± 0.392

5.099 ± 0.314

5.388 ± 0.501

4.201 ± 0.779

3.72 ± 0.343

8.146 ± 0.61

6.414 ± 0.706

11.482 ± 0.951

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.324

5.484 ± 0.449

5.516 ± 0.266

3.624 ± 0.192

4.009 ± 0.422

9.397 ± 1.025

5.901 ± 0.269

4.201 ± 0.402

1.668 ± 0.402

4.041 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski