Mycobacterium phage Bartholomew

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; Fishburnevirus; unclassified Fishburnevirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222ZQL0|A0A222ZQL0_9CAUD RecT-like ssDNA binding protein OS=Mycobacterium phage Bartholomew OX=2015875 GN=46 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.47YY4 pKa = 11.31LDD6 pKa = 4.12ASGLTDD12 pKa = 4.02AGLPDD17 pKa = 4.67EE18 pKa = 5.45YY19 pKa = 11.43LDD21 pKa = 6.24LDD23 pKa = 4.75DD24 pKa = 6.48LDD26 pKa = 5.03LPRR29 pKa = 11.84STAPQCEE36 pKa = 4.53HH37 pKa = 7.06PPVACTAEE45 pKa = 4.19CRR47 pKa = 11.84GVRR50 pKa = 11.84AGSTVARR57 pKa = 11.84FLVTLGTTLVAFALVLALTLGAVLAA82 pKa = 4.94

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222ZPR6|A0A222ZPR6_9CAUD Tail terminator OS=Mycobacterium phage Bartholomew OX=2015875 GN=13 PE=4 SV=1
MM1 pKa = 6.89VNRR4 pKa = 11.84WRR6 pKa = 11.84TWQFAQSLSARR17 pKa = 11.84TVDD20 pKa = 3.31EE21 pKa = 3.98RR22 pKa = 11.84VATVRR27 pKa = 11.84RR28 pKa = 11.84MAVWCGVEE36 pKa = 3.95PEE38 pKa = 4.35FAQVEE43 pKa = 4.91HH44 pKa = 6.35IVSWLAEE51 pKa = 4.26GGQWSARR58 pKa = 11.84TRR60 pKa = 11.84WTYY63 pKa = 11.34YY64 pKa = 10.31GALSAWFLWLQQQGHH79 pKa = 5.14RR80 pKa = 11.84HH81 pKa = 6.2DD82 pKa = 4.56NPMVMIGRR90 pKa = 11.84PKK92 pKa = 10.09RR93 pKa = 11.84PKK95 pKa = 9.75SVPRR99 pKa = 11.84PVSNLDD105 pKa = 3.3MQRR108 pKa = 11.84LLAVRR113 pKa = 11.84AHH115 pKa = 6.47KK116 pKa = 9.25RR117 pKa = 11.84TKK119 pKa = 11.33AMILLAAFQGLRR131 pKa = 11.84VHH133 pKa = 7.38EE134 pKa = 4.11IAQIKK139 pKa = 10.05GEE141 pKa = 4.07HH142 pKa = 6.8LDD144 pKa = 4.12LIEE147 pKa = 4.13RR148 pKa = 11.84TMTVTGKK155 pKa = 11.13GNVTATLPLHH165 pKa = 5.96HH166 pKa = 6.79RR167 pKa = 11.84VVEE170 pKa = 4.15IAYY173 pKa = 8.39QMPRR177 pKa = 11.84KK178 pKa = 9.34GHH180 pKa = 6.4WFPGPDD186 pKa = 2.81RR187 pKa = 11.84GHH189 pKa = 5.51QRR191 pKa = 11.84RR192 pKa = 11.84EE193 pKa = 4.31SVSGTIKK200 pKa = 10.28EE201 pKa = 4.01AMIRR205 pKa = 11.84AGVVGSAHH213 pKa = 7.08CLRR216 pKa = 11.84HH217 pKa = 4.94WFGTALLEE225 pKa = 4.69AGVDD229 pKa = 3.56LRR231 pKa = 11.84TVQEE235 pKa = 4.11LMRR238 pKa = 11.84HH239 pKa = 4.7QSLTSTEE246 pKa = 3.62IYY248 pKa = 10.3TRR250 pKa = 11.84VTDD253 pKa = 3.31QRR255 pKa = 11.84RR256 pKa = 11.84AEE258 pKa = 4.58GIEE261 pKa = 4.09RR262 pKa = 11.84IDD264 pKa = 3.88PFRR267 pKa = 11.84VAPSTRR273 pKa = 11.84VSDD276 pKa = 3.72RR277 pKa = 11.84LLAQIAEE284 pKa = 4.42PVEE287 pKa = 4.59GAAGAAVSAAA297 pKa = 3.56

Molecular weight: 33.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14973

28

1256

194.5

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.302 ± 0.528

0.975 ± 0.123

6.712 ± 0.183

6.118 ± 0.342

2.818 ± 0.171

8.949 ± 0.535

1.97 ± 0.157

4.281 ± 0.139

3.039 ± 0.21

7.914 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.024 ± 0.116

2.992 ± 0.172

6.211 ± 0.215

3.64 ± 0.249

7.286 ± 0.424

5.31 ± 0.288

6.285 ± 0.262

6.919 ± 0.358

1.937 ± 0.128

2.318 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski