Flavobacterium phage vB_FspS_tant8-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tantvirus; Flavobacterium virus Tant

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LG39|A0A6B9LG39_9CAUD Terminase large subunit OS=Flavobacterium phage vB_FspS_tant8-1 OX=2686278 GN=tant81_gp036 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.51HH3 pKa = 5.49FTFKK7 pKa = 11.36DD8 pKa = 3.15NDD10 pKa = 3.71ALDD13 pKa = 4.07LFKK16 pKa = 11.21KK17 pKa = 9.46LTQQKK22 pKa = 10.37DD23 pKa = 3.22IEE25 pKa = 4.16QHH27 pKa = 6.41IMEE30 pKa = 4.23QLFEE34 pKa = 4.22RR35 pKa = 11.84FEE37 pKa = 4.35IPFDD41 pKa = 3.79DD42 pKa = 4.71CFGHH46 pKa = 7.03SIQTILDD53 pKa = 3.63DD54 pKa = 4.23EE55 pKa = 5.59KK56 pKa = 10.96IDD58 pKa = 4.0SLEE61 pKa = 3.83FLQLAFPTLKK71 pKa = 10.54NYY73 pKa = 10.85SEE75 pKa = 5.04LIEE78 pKa = 4.34ILSSIIFFGTDD89 pKa = 3.15EE90 pKa = 4.53NPCPEE95 pKa = 5.05CGCEE99 pKa = 5.31LDD101 pKa = 4.51IEE103 pKa = 4.53NDD105 pKa = 3.63GSGSYY110 pKa = 10.14SWTNINCTFCDD121 pKa = 3.39YY122 pKa = 11.72GEE124 pKa = 4.19TDD126 pKa = 3.7EE127 pKa = 5.69PDD129 pKa = 3.42WDD131 pKa = 4.41CLPGGHH137 pKa = 7.51DD138 pKa = 4.64DD139 pKa = 3.92YY140 pKa = 12.2

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LNM3|A0A6B9LNM3_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_tant8-1 OX=2686278 GN=tant81_gp010 PE=4 SV=1
MM1 pKa = 7.46SKK3 pKa = 9.83IFHH6 pKa = 6.75KK7 pKa = 10.78VFKK10 pKa = 10.71CLFAKK15 pKa = 10.23RR16 pKa = 11.84HH17 pKa = 5.53RR18 pKa = 11.84SSGGVFLFYY27 pKa = 10.54SIKK30 pKa = 10.39KK31 pKa = 9.6

Molecular weight: 3.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13239

31

1087

213.5

24.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.958 ± 0.348

0.967 ± 0.118

6.126 ± 0.249

8.09 ± 0.437

6.556 ± 0.322

5.197 ± 0.338

1.292 ± 0.141

8.558 ± 0.354

9.918 ± 0.625

8.067 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.174

7.229 ± 0.298

2.644 ± 0.218

4.253 ± 0.266

3.709 ± 0.254

6.292 ± 0.249

5.756 ± 0.306

5.469 ± 0.249

1.065 ± 0.134

2.719 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski