Hyaloscypha hepaticicola

Taxonomy:

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2J6Q879|A0A2J6Q879_9HELO Geranylgeranyl transferase type-2 subunit alpha OS=Hyaloscypha hepaticicola OX=2082293 GN=NA56DRAFT_624053 PE=3 SV=1
AA1 pKa = 7.57LFATTILAAPRR12 pKa = 11.84GNGFAHH18 pKa = 6.75RR19 pKa = 11.84AARR22 pKa = 11.84RR23 pKa = 11.84ATTRR27 pKa = 11.84TGGLADD33 pKa = 3.52IKK35 pKa = 10.97GHH37 pKa = 6.44EE38 pKa = 4.56DD39 pKa = 3.29LFNPEE44 pKa = 4.15TGNTTHH50 pKa = 5.86VQYY53 pKa = 11.09SSNWAGAVITSPPSGEE69 pKa = 3.89TFSFVQGQFVVPTPSLPAGSAAIWVGIDD97 pKa = 2.83GDD99 pKa = 4.26TYY101 pKa = 11.85GNAILQAGVDD111 pKa = 3.65VTVNSDD117 pKa = 2.57GSTSYY122 pKa = 11.22DD123 pKa = 2.84SWYY126 pKa = 10.04EE127 pKa = 3.49WYY129 pKa = 8.6PQYY132 pKa = 11.08SITIDD137 pKa = 3.31PSQFSFGAGDD147 pKa = 3.66TVFVSVQATDD157 pKa = 3.52STDD160 pKa = 3.11GTVILQNLSTGQSTTQTVSAPDD182 pKa = 3.33SSSALGGQNAEE193 pKa = 4.3WIVEE197 pKa = 4.02DD198 pKa = 4.44FEE200 pKa = 5.83EE201 pKa = 4.84NGSQVAFTNFGSVSFTNTVAQTAQGSQLGNVITQVSIPSSSEE243 pKa = 3.48VDD245 pKa = 3.16VTYY248 pKa = 11.28

Molecular weight: 25.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2J6QPK8|A0A2J6QPK8_9HELO LamB/YcsF OS=Hyaloscypha hepaticicola OX=2082293 GN=NA56DRAFT_3509 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.44QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16778

0

16778

7082214

49

5356

422.1

46.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.001 ± 0.018

1.394 ± 0.008

5.316 ± 0.012

6.34 ± 0.019

3.908 ± 0.012

6.77 ± 0.018

2.322 ± 0.009

5.34 ± 0.015

5.163 ± 0.016

9.128 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.007

3.855 ± 0.009

5.824 ± 0.022

3.932 ± 0.013

5.886 ± 0.016

8.393 ± 0.023

5.986 ± 0.015

5.896 ± 0.012

1.553 ± 0.008

2.818 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski