Avon-Heathcote Estuary associated circular virus 6

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBI9|A0A0C5IBI9_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 6 OX=1618257 PE=3 SV=1
MM1 pKa = 7.17SRR3 pKa = 11.84SRR5 pKa = 11.84NWCFTLHH12 pKa = 6.85NYY14 pKa = 9.15TDD16 pKa = 4.09DD17 pKa = 5.34DD18 pKa = 4.0IEE20 pKa = 3.91IFKK23 pKa = 10.6NIKK26 pKa = 9.07CRR28 pKa = 11.84YY29 pKa = 7.63IIYY32 pKa = 10.09GKK34 pKa = 9.18EE35 pKa = 3.33ICPTTKK41 pKa = 9.88RR42 pKa = 11.84QHH44 pKa = 5.2LQGYY48 pKa = 8.21IQFDD52 pKa = 3.59NQRR55 pKa = 11.84TITAVKK61 pKa = 10.13KK62 pKa = 10.5FLGLPKK68 pKa = 9.95IHH70 pKa = 7.29LEE72 pKa = 3.92IANGSSDD79 pKa = 4.57DD80 pKa = 3.79NKK82 pKa = 10.59TYY84 pKa = 10.49CSKK87 pKa = 11.13DD88 pKa = 2.49GDD90 pKa = 3.73MYY92 pKa = 11.43EE93 pKa = 5.73RR94 pKa = 11.84GDD96 pKa = 3.75CKK98 pKa = 10.5QQGRR102 pKa = 11.84RR103 pKa = 11.84TDD105 pKa = 2.95ITDD108 pKa = 3.29IKK110 pKa = 11.65DD111 pKa = 3.61MISNGATMRR120 pKa = 11.84DD121 pKa = 4.1IIPHH125 pKa = 5.29ATSVQSVRR133 pKa = 11.84MAEE136 pKa = 3.62IHH138 pKa = 6.56LKK140 pKa = 10.5YY141 pKa = 10.43SEE143 pKa = 4.7QKK145 pKa = 9.32RR146 pKa = 11.84TWKK149 pKa = 10.4PKK151 pKa = 8.07VQWFYY156 pKa = 11.71GPTGTGKK163 pKa = 8.11TRR165 pKa = 11.84TAYY168 pKa = 9.86EE169 pKa = 4.11ILEE172 pKa = 4.46DD173 pKa = 4.79PYY175 pKa = 9.42TTLDD179 pKa = 3.12TGQWWEE185 pKa = 4.65GYY187 pKa = 8.95DD188 pKa = 3.2AHH190 pKa = 6.79EE191 pKa = 4.34NVIIDD196 pKa = 4.0DD197 pKa = 3.74MRR199 pKa = 11.84GDD201 pKa = 3.53FMKK204 pKa = 10.99YY205 pKa = 9.71HH206 pKa = 6.27VLLKK210 pKa = 10.97LLDD213 pKa = 3.82RR214 pKa = 11.84YY215 pKa = 10.95AYY217 pKa = 9.52IVEE220 pKa = 4.4CKK222 pKa = 10.35GGSRR226 pKa = 11.84QFLARR231 pKa = 11.84HH232 pKa = 6.25IIITSAFHH240 pKa = 6.54PKK242 pKa = 10.36DD243 pKa = 3.55VFHH246 pKa = 6.44TRR248 pKa = 11.84EE249 pKa = 4.8DD250 pKa = 3.19IAQLMRR256 pKa = 11.84RR257 pKa = 11.84IDD259 pKa = 3.59CVKK262 pKa = 10.24EE263 pKa = 4.68FKK265 pKa = 10.82

Molecular weight: 31.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBI9|A0A0C5IBI9_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 6 OX=1618257 PE=3 SV=1
MM1 pKa = 7.52PPIRR5 pKa = 11.84KK6 pKa = 9.29LDD8 pKa = 3.66TRR10 pKa = 11.84AKK12 pKa = 9.72RR13 pKa = 11.84AAALRR18 pKa = 11.84RR19 pKa = 11.84GQIRR23 pKa = 11.84APRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84GAPFRR33 pKa = 11.84YY34 pKa = 8.55RR35 pKa = 11.84TGTKK39 pKa = 9.86RR40 pKa = 11.84GRR42 pKa = 11.84TTAARR47 pKa = 11.84RR48 pKa = 11.84NGNTIALTTLSRR60 pKa = 11.84KK61 pKa = 8.65QEE63 pKa = 4.04NLSISYY69 pKa = 10.75RR70 pKa = 11.84EE71 pKa = 3.73MLEE74 pKa = 4.01FNDD77 pKa = 3.69MGGDD81 pKa = 3.61NGSTPCLIRR90 pKa = 11.84VNLNNPVIGGATPTGPDD107 pKa = 3.39TIVSVVGNLKK117 pKa = 9.46TGATDD122 pKa = 3.33PTFIPHH128 pKa = 6.36SYY130 pKa = 9.33EE131 pKa = 3.8NKK133 pKa = 9.95RR134 pKa = 11.84NLSDD138 pKa = 3.66RR139 pKa = 11.84LAEE142 pKa = 3.95YY143 pKa = 10.37FSIYY147 pKa = 8.66RR148 pKa = 11.84TAIVTSAEE156 pKa = 4.19VTVVCTPKK164 pKa = 10.62LNQLNGMTGNNRR176 pKa = 11.84SIVPFTQNRR185 pKa = 11.84ASEE188 pKa = 4.36DD189 pKa = 3.53PAVAGQATYY198 pKa = 10.5LYY200 pKa = 9.73QHH202 pKa = 6.89NANAMPQVEE211 pKa = 4.64VWSIRR216 pKa = 11.84QQNQGQLTSAAAGSPPLEE234 pKa = 4.22TLKK237 pKa = 10.84QGIPGMRR244 pKa = 11.84MTRR247 pKa = 11.84LNVVPNSTKK256 pKa = 10.13GVTYY260 pKa = 10.74KK261 pKa = 10.09MSYY264 pKa = 8.16TPKK267 pKa = 10.56SMYY270 pKa = 10.55AISDD274 pKa = 3.21WKK276 pKa = 11.0DD277 pKa = 3.04NKK279 pKa = 10.45KK280 pKa = 10.0VLKK283 pKa = 10.3IFNNAVKK290 pKa = 10.84NPDD293 pKa = 3.11QKK295 pKa = 10.48EE296 pKa = 3.66AYY298 pKa = 8.76MYY300 pKa = 10.82VGIAGRR306 pKa = 11.84FQGLDD311 pKa = 3.45PVASLANMGLPHH323 pKa = 6.72FNVEE327 pKa = 4.19VKK329 pKa = 10.54VKK331 pKa = 10.45YY332 pKa = 10.38NINFSEE338 pKa = 4.94RR339 pKa = 11.84YY340 pKa = 10.02NIDD343 pKa = 3.3GNNEE347 pKa = 4.08PVPHH351 pKa = 6.61TEE353 pKa = 3.76LL354 pKa = 5.19

Molecular weight: 39.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

619

265

354

309.5

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.462 ± 1.511

1.454 ± 0.776

5.654 ± 1.978

4.362 ± 0.356

3.069 ± 0.46

6.785 ± 0.489

2.262 ± 0.989

6.947 ± 1.626

6.624 ± 1.097

5.977 ± 0.454

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.069 ± 0.033

6.462 ± 2.251

5.008 ± 1.547

4.2 ± 0.214

7.593 ± 0.277

5.008 ± 0.56

8.078 ± 0.1

5.331 ± 1.265

1.131 ± 0.494

4.523 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski