Wuhan spider virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG33|A0A1L3KG33_9VIRU RNA-directed RNA polymerase OS=Wuhan spider virus 9 OX=1923758 PE=4 SV=1
MM1 pKa = 7.14HH2 pKa = 7.62AIVVTDD8 pKa = 5.32HH9 pKa = 6.32IWTNYY14 pKa = 8.17GRR16 pKa = 11.84YY17 pKa = 9.18AATAQSFINQQRR29 pKa = 11.84ALYY32 pKa = 9.98DD33 pKa = 3.76PNDD36 pKa = 4.39RR37 pKa = 11.84AWHH40 pKa = 6.53IAPQHH45 pKa = 5.28QPLAIVAHH53 pKa = 6.6DD54 pKa = 3.94WQWDD58 pKa = 3.39ATYY61 pKa = 10.51RR62 pKa = 11.84CYY64 pKa = 10.55ISNLDD69 pKa = 3.59YY70 pKa = 11.21SWAVDD75 pKa = 3.72YY76 pKa = 7.71THH78 pKa = 7.52KK79 pKa = 10.46ISPQFWFSVHH89 pKa = 4.94MPKK92 pKa = 10.43GIAMPVVGAVGSDD105 pKa = 2.72WDD107 pKa = 4.66VIQHH111 pKa = 7.0WIHH114 pKa = 5.95GVPVITHH121 pKa = 6.66IEE123 pKa = 4.1DD124 pKa = 3.35QVSRR128 pKa = 11.84GAKK131 pKa = 9.42IGGVEE136 pKa = 4.0YY137 pKa = 10.57NYY139 pKa = 10.81TDD141 pKa = 4.56LSINLQLTIPKK152 pKa = 9.62HH153 pKa = 4.61SQNFRR158 pKa = 11.84INLPLITLYY167 pKa = 10.33PDD169 pKa = 3.02TPYY172 pKa = 10.5IITVAATFYY181 pKa = 10.49MFTLEE186 pKa = 4.1RR187 pKa = 11.84AKK189 pKa = 10.6KK190 pKa = 8.11EE191 pKa = 3.83QPGFYY196 pKa = 10.41NLAEE200 pKa = 3.76VWFYY204 pKa = 11.39QEE206 pKa = 4.84GGAPDD211 pKa = 3.59EE212 pKa = 4.47PSRR215 pKa = 4.48

Molecular weight: 24.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGI4|A0A1L3KGI4_9VIRU Uncharacterized protein OS=Wuhan spider virus 9 OX=1923758 PE=4 SV=1
MM1 pKa = 7.05MNQYY5 pKa = 10.25RR6 pKa = 11.84GRR8 pKa = 11.84GRR10 pKa = 11.84FWNAGLNLAAAAGGAAARR28 pKa = 11.84VAANRR33 pKa = 11.84FQNWVNSPPSNPYY46 pKa = 10.44NMQPIYY52 pKa = 10.27QALPPVRR59 pKa = 11.84RR60 pKa = 11.84GRR62 pKa = 11.84GRR64 pKa = 11.84GRR66 pKa = 11.84GRR68 pKa = 11.84GRR70 pKa = 11.84GRR72 pKa = 11.84GRR74 pKa = 11.84PQAVTPGRR82 pKa = 11.84QPSAGISQTRR92 pKa = 11.84SGAKK96 pKa = 9.41ISIRR100 pKa = 11.84DD101 pKa = 3.44TEE103 pKa = 4.33AFVVEE108 pKa = 4.51QGFKK112 pKa = 10.2AYY114 pKa = 9.98KK115 pKa = 9.34MNPAVNQLPRR125 pKa = 11.84LEE127 pKa = 4.34QHH129 pKa = 5.43SKK131 pKa = 8.2MYY133 pKa = 9.61KK134 pKa = 9.5RR135 pKa = 11.84YY136 pKa = 9.56RR137 pKa = 11.84IRR139 pKa = 11.84YY140 pKa = 7.83FNIAYY145 pKa = 9.77KK146 pKa = 10.35SGSGTATSGNVTVGILGGTIDD167 pKa = 4.95SKK169 pKa = 10.32VTSKK173 pKa = 11.14NVINLRR179 pKa = 11.84PSFFTPAWKK188 pKa = 10.61NEE190 pKa = 3.59TMSLGSDD197 pKa = 2.72IDD199 pKa = 3.18IARR202 pKa = 11.84WMLCEE207 pKa = 4.28QDD209 pKa = 4.85DD210 pKa = 4.04NDD212 pKa = 4.97GIAFTLYY219 pKa = 10.87VNSTEE224 pKa = 4.7AGLGMIQCSYY234 pKa = 10.58EE235 pKa = 3.93VEE237 pKa = 4.17FDD239 pKa = 3.44QPHH242 pKa = 7.15PFTT245 pKa = 4.91

Molecular weight: 27.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1502

215

558

375.5

42.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.858 ± 0.804

1.664 ± 0.501

4.927 ± 0.793

5.06 ± 0.654

3.928 ± 0.182

6.125 ± 0.896

2.796 ± 0.54

6.525 ± 0.548

5.26 ± 1.018

8.389 ± 1.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.397

3.995 ± 0.647

5.792 ± 0.81

4.461 ± 0.411

7.057 ± 0.727

5.792 ± 0.89

6.059 ± 0.251

5.992 ± 0.331

2.197 ± 0.454

4.461 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski