Streptomyces phage Paedore

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JTM9|A0A2P1JTM9_9CAUD Tail assembly chaperone OS=Streptomyces phage Paedore OX=2108134 GN=17 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.72AATLVIGSGNYY16 pKa = 7.79LTAPVGTDD24 pKa = 2.71IPTDD28 pKa = 3.63LLVPVSPWEE37 pKa = 4.27AVGHH41 pKa = 5.85TSLEE45 pKa = 4.62DD46 pKa = 3.05IFSITSEE53 pKa = 4.1GGEE56 pKa = 3.93ATTIGSLQNKK66 pKa = 7.29SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.14TMAFTLQQFDD87 pKa = 3.92VKK89 pKa = 10.73GLKK92 pKa = 9.98LYY94 pKa = 10.4YY95 pKa = 10.42GSNAPILPDD104 pKa = 3.48GSVGVPTDD112 pKa = 3.82PVPTTAAFLAVFVDD126 pKa = 4.2GEE128 pKa = 4.59NVFAFYY134 pKa = 10.79APKK137 pKa = 10.7AEE139 pKa = 4.43IYY141 pKa = 10.57RR142 pKa = 11.84GDD144 pKa = 3.76DD145 pKa = 3.2VSFGDD150 pKa = 3.91TEE152 pKa = 4.44SLAGLPLGVKK162 pKa = 10.04PMAYY166 pKa = 9.81GANAWTYY173 pKa = 11.02AITPLGTVASTGATAGTPGSFTPAGSTAPYY203 pKa = 10.82DD204 pKa = 4.58LVDD207 pKa = 4.72LDD209 pKa = 5.35LVTAEE214 pKa = 5.24PKK216 pKa = 9.37TAWTTGQYY224 pKa = 10.36VVLGDD229 pKa = 3.93GSNAYY234 pKa = 8.94WDD236 pKa = 3.99GADD239 pKa = 3.15WATGIAAA246 pKa = 4.68

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JTS3|A0A2P1JTS3_9CAUD DNA binding protein OS=Streptomyces phage Paedore OX=2108134 GN=46 PE=4 SV=1
MM1 pKa = 7.38IRR3 pKa = 11.84RR4 pKa = 11.84AVSRR8 pKa = 11.84AAGPLFTFLVLASIGLVIGMAIIGRR33 pKa = 11.84PTPP36 pKa = 3.58

Molecular weight: 3.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

15728

36

1061

187.2

20.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.394 ± 0.394

0.757 ± 0.13

6.358 ± 0.245

7.191 ± 0.453

3.052 ± 0.174

8.711 ± 0.41

2.168 ± 0.191

4.292 ± 0.27

4.349 ± 0.265

8.17 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.157

2.651 ± 0.144

4.718 ± 0.208

3.236 ± 0.159

6.651 ± 0.402

5.869 ± 0.239

6.434 ± 0.313

7.14 ± 0.25

1.78 ± 0.127

2.817 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski