Salinibacter ruber (strain DSM 13855 / M31)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidetes Order II. Incertae sedis; Rhodothermaceae; Salinibacter; Salinibacter ruber

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2S290|Q2S290_SALRD Uncharacterized protein OS=Salinibacter ruber (strain DSM 13855 / M31) OX=309807 GN=SRU_1570 PE=4 SV=1
MM1 pKa = 7.55SSDD4 pKa = 3.78PAPTDD9 pKa = 3.7TSPSWSDD16 pKa = 2.47RR17 pKa = 11.84WLNRR21 pKa = 11.84IEE23 pKa = 4.13RR24 pKa = 11.84TGNALPDD31 pKa = 3.81PVTLFFIFIAIVMVASWITHH51 pKa = 4.95TADD54 pKa = 2.85VSVVHH59 pKa = 6.94PGTDD63 pKa = 3.3EE64 pKa = 4.61TITADD69 pKa = 4.28NLFSDD74 pKa = 4.02EE75 pKa = 4.18NIRR78 pKa = 11.84RR79 pKa = 11.84LFTDD83 pKa = 3.18MAEE86 pKa = 4.36TFADD90 pKa = 5.38FPPLGLVLVVMLGIGVADD108 pKa = 3.47KK109 pKa = 10.88TGLISAALKK118 pKa = 10.74SFVASVPDD126 pKa = 3.93ALLTAALVFAGIMSSLAVDD145 pKa = 3.63AGYY148 pKa = 11.12VVVIPLGAVLFYY160 pKa = 11.12GVGRR164 pKa = 11.84HH165 pKa = 5.62PLAGLGAAFAGVSAGFSANLLLTSLDD191 pKa = 3.58PLLASFTEE199 pKa = 4.44PAAQLIAEE207 pKa = 4.95DD208 pKa = 3.96YY209 pKa = 10.35SVPVTANWYY218 pKa = 10.86LMIALTPVFVVLGAYY233 pKa = 8.07ITDD236 pKa = 4.7TIVEE240 pKa = 4.63PYY242 pKa = 9.9LGEE245 pKa = 4.14YY246 pKa = 9.11EE247 pKa = 4.16PPEE250 pKa = 4.64DD251 pKa = 4.67FDD253 pKa = 5.18EE254 pKa = 4.65EE255 pKa = 4.21DD256 pKa = 3.97AEE258 pKa = 4.63SSEE261 pKa = 4.15LTDD264 pKa = 3.25EE265 pKa = 4.24EE266 pKa = 4.62RR267 pKa = 11.84RR268 pKa = 11.84GLRR271 pKa = 11.84WAGGVTLASILGVVLLAVPEE291 pKa = 4.28GAPLAEE297 pKa = 4.46FEE299 pKa = 4.67ALIEE303 pKa = 4.51SIVALMVFLFFLPGLTYY320 pKa = 10.55GIVVGEE326 pKa = 4.54IEE328 pKa = 5.15DD329 pKa = 4.43DD330 pKa = 3.8SDD332 pKa = 3.99VADD335 pKa = 3.93MMADD339 pKa = 3.24SMADD343 pKa = 3.01MGAYY347 pKa = 9.0IVLAFAAAHH356 pKa = 6.34FIAMFEE362 pKa = 4.03WSNLGSIIAISGADD376 pKa = 3.41ALQSIGFTGLPLLFSFIFVSALINLFVGSASAKK409 pKa = 7.0WAIMAPVFVPMLMLAGDD426 pKa = 4.15PGYY429 pKa = 10.92SPEE432 pKa = 4.29TVQAAYY438 pKa = 10.51RR439 pKa = 11.84IGDD442 pKa = 3.78SFTNILTPLLPYY454 pKa = 9.98FPLVIIFAQRR464 pKa = 11.84YY465 pKa = 9.76DD466 pKa = 3.63EE467 pKa = 4.6DD468 pKa = 4.9AGIGSIIALMLPYY481 pKa = 10.28SVGFGVVSTIVFLVWVLLGLPLGPGAEE508 pKa = 4.26LYY510 pKa = 11.56YY511 pKa = 10.33MGG513 pKa = 5.26

Molecular weight: 54.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2RYZ6|Q2RYZ6_SALRD Glycosyl hydrolase family 13 OS=Salinibacter ruber (strain DSM 13855 / M31) OX=309807 GN=SRU_2743 PE=4 SV=1
MM1 pKa = 7.91PPRR4 pKa = 11.84PTLRR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.18EE11 pKa = 4.19WTRR14 pKa = 11.84TRR16 pKa = 11.84NGEE19 pKa = 4.19VPRR22 pKa = 11.84HH23 pKa = 5.37FSLPSRR29 pKa = 11.84AGAAAHH35 pKa = 5.93EE36 pKa = 4.54ARR38 pKa = 11.84VSLAVAVEE46 pKa = 4.17EE47 pKa = 4.21AWARR51 pKa = 11.84CCRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84PGRR59 pKa = 11.84EE60 pKa = 3.43RR61 pKa = 11.84LSPCHH66 pKa = 6.91DD67 pKa = 3.52GDD69 pKa = 4.49EE70 pKa = 4.67EE71 pKa = 4.67GTKK74 pKa = 9.58DD75 pKa = 3.21TAVRR79 pKa = 11.84ASARR83 pKa = 11.84GQPRR87 pKa = 11.84RR88 pKa = 11.84SCTPADD94 pKa = 3.75SRR96 pKa = 11.84ILAPTVARR104 pKa = 11.84VMSTSPDD111 pKa = 3.54DD112 pKa = 3.6NRR114 pKa = 11.84VWRR117 pKa = 11.84VFYY120 pKa = 10.54TRR122 pKa = 11.84ARR124 pKa = 11.84AEE126 pKa = 4.41KK127 pKa = 10.09KK128 pKa = 10.5CEE130 pKa = 3.72TRR132 pKa = 11.84LDD134 pKa = 3.5EE135 pKa = 4.36RR136 pKa = 11.84RR137 pKa = 11.84IDD139 pKa = 3.53VMVPKK144 pKa = 9.51KK145 pKa = 9.54TEE147 pKa = 3.47VRR149 pKa = 11.84QWSDD153 pKa = 2.6RR154 pKa = 11.84TKK156 pKa = 10.78EE157 pKa = 3.48ITEE160 pKa = 3.95PLFRR164 pKa = 11.84NYY166 pKa = 10.46LFARR170 pKa = 11.84VDD172 pKa = 3.29EE173 pKa = 4.35KK174 pKa = 11.53DD175 pKa = 3.07RR176 pKa = 11.84LRR178 pKa = 11.84VLRR181 pKa = 11.84TNGIVRR187 pKa = 11.84CVHH190 pKa = 6.66FDD192 pKa = 3.59GEE194 pKa = 4.31PARR197 pKa = 11.84LRR199 pKa = 11.84EE200 pKa = 4.08EE201 pKa = 4.2TVDD204 pKa = 3.66RR205 pKa = 11.84LQKK208 pKa = 10.36AQAVPEE214 pKa = 4.17RR215 pKa = 11.84LSTADD220 pKa = 3.46LRR222 pKa = 11.84PAVGEE227 pKa = 4.27TVTITGGPEE236 pKa = 3.57RR237 pKa = 11.84LQGLTGKK244 pKa = 9.52VLQHH248 pKa = 6.91RR249 pKa = 11.84GQTYY253 pKa = 10.59LLVQVRR259 pKa = 11.84AVRR262 pKa = 11.84QAVKK266 pKa = 10.45IEE268 pKa = 3.98VAADD272 pKa = 3.6WVQTTSNTT280 pKa = 3.44

Molecular weight: 31.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2812

0

2812

1007991

30

2597

358.5

39.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.703 ± 0.069

0.683 ± 0.012

7.015 ± 0.052

7.032 ± 0.07

3.394 ± 0.025

8.37 ± 0.046

2.227 ± 0.026

3.71 ± 0.03

2.105 ± 0.028

9.689 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.911 ± 0.018

2.451 ± 0.031

5.653 ± 0.038

3.549 ± 0.026

7.716 ± 0.06

5.833 ± 0.04

6.22 ± 0.03

7.872 ± 0.042

1.251 ± 0.018

2.617 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski