Anaerohalosphaera lusitana

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; Sedimentisphaerales; Anaerohalosphaeraceae; Anaerohalosphaera

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9NN70|A0A1U9NN70_9BACT Uncharacterized protein OS=Anaerohalosphaera lusitana OX=1936003 GN=STSP2_02436 PE=4 SV=1
MM1 pKa = 7.34SFKK4 pKa = 10.68KK5 pKa = 10.79SFVPVVNLGRR15 pKa = 11.84IMRR18 pKa = 11.84RR19 pKa = 11.84CSKK22 pKa = 10.29LFAIIAILLFVSQAGYY38 pKa = 11.14ASLVGHH44 pKa = 7.19WKK46 pKa = 10.47LDD48 pKa = 3.89DD49 pKa = 4.48GSGSTAASAVDD60 pKa = 3.85SPADD64 pKa = 3.77DD65 pKa = 3.91GAIVGTAAWEE75 pKa = 4.37STDD78 pKa = 3.36IPPVPGGNSYY88 pKa = 11.25ALEE91 pKa = 4.06LAAGKK96 pKa = 10.81GIDD99 pKa = 3.84TPVNGIPGAGGRR111 pKa = 11.84TISAWINVDD120 pKa = 3.15PTASLAEE127 pKa = 4.3GGGGIVTWGDD137 pKa = 2.52NWANGLGHH145 pKa = 7.45RR146 pKa = 11.84FTFKK150 pKa = 10.33IDD152 pKa = 3.54DD153 pKa = 3.83SSTGRR158 pKa = 11.84LRR160 pKa = 11.84VEE162 pKa = 3.59IGGGYY167 pKa = 10.41AVGSTSVNDD176 pKa = 4.64GQWHH180 pKa = 5.64HH181 pKa = 6.32VAVTLADD188 pKa = 4.09GQSSVANVKK197 pKa = 9.94FYY199 pKa = 10.67IDD201 pKa = 3.91GEE203 pKa = 4.25VDD205 pKa = 3.81VISDD209 pKa = 4.04SDD211 pKa = 3.6TSNPINSDD219 pKa = 3.2AAPIDD224 pKa = 3.18IGYY227 pKa = 10.11CEE229 pKa = 4.94ALLDD233 pKa = 3.86TWSKK237 pKa = 11.61AMGIEE242 pKa = 4.11GGIDD246 pKa = 3.26DD247 pKa = 4.19VRR249 pKa = 11.84VYY251 pKa = 11.07DD252 pKa = 3.97HH253 pKa = 6.96EE254 pKa = 4.51LSEE257 pKa = 4.98GEE259 pKa = 4.41ILLLCTGSDD268 pKa = 3.33EE269 pKa = 4.65PYY271 pKa = 10.81VYY273 pKa = 10.61GPSPGDD279 pKa = 3.73GEE281 pKa = 5.05DD282 pKa = 3.49HH283 pKa = 6.66VSPDD287 pKa = 4.42AVLSWSAGAVEE298 pKa = 4.41NPDD301 pKa = 3.24YY302 pKa = 11.04DD303 pKa = 4.41VNIGTTMACDD313 pKa = 4.01EE314 pKa = 5.04IISGVSTGSATSYY327 pKa = 10.83VPAAGTLTLGNTYY340 pKa = 9.23FWRR343 pKa = 11.84VDD345 pKa = 3.35VTDD348 pKa = 4.4GGTEE352 pKa = 3.81YY353 pKa = 10.62TGDD356 pKa = 3.03VWSFTVGAGSATDD369 pKa = 3.81PVPADD374 pKa = 3.48GASIVATSAYY384 pKa = 10.62LDD386 pKa = 3.32WTGDD390 pKa = 3.77DD391 pKa = 4.52FVDD394 pKa = 3.98SYY396 pKa = 11.92RR397 pKa = 11.84IMFAPAGEE405 pKa = 4.07QLVNAGEE412 pKa = 4.24YY413 pKa = 10.52AGAPVGLAKK422 pKa = 10.17IARR425 pKa = 11.84AAGMDD430 pKa = 3.84LLEE433 pKa = 5.11AGTYY437 pKa = 9.17DD438 pKa = 3.47WQVVSLDD445 pKa = 3.73ASGEE449 pKa = 4.25MINSGPVWSFSIGTDD464 pKa = 2.07IWVYY468 pKa = 10.32MLGDD472 pKa = 4.0YY473 pKa = 11.08VPVDD477 pKa = 3.39TTVDD481 pKa = 3.49DD482 pKa = 4.16FFSYY486 pKa = 11.14VSTADD491 pKa = 5.25LLGTWTDD498 pKa = 3.71GAANGSNAQATIDD511 pKa = 3.65AMEE514 pKa = 4.4GAVTLSYY521 pKa = 11.67DD522 pKa = 3.52NTTVPYY528 pKa = 10.04QSYY531 pKa = 10.45ISRR534 pKa = 11.84TFASVQDD541 pKa = 3.59WSGEE545 pKa = 4.05QVLSVTMQGDD555 pKa = 3.71SANAGEE561 pKa = 4.3TLFVGLSDD569 pKa = 4.04GTDD572 pKa = 3.43SADD575 pKa = 4.46LALQSEE581 pKa = 4.71KK582 pKa = 10.85LVSNDD587 pKa = 2.76EE588 pKa = 4.13ATTITLALSEE598 pKa = 4.16FAAAGVDD605 pKa = 3.69LANVSEE611 pKa = 4.02MRR613 pKa = 11.84IGTGDD618 pKa = 3.43GSTGGAAGSIVVYY631 pKa = 10.73DD632 pKa = 4.2VMIHH636 pKa = 6.11PAGSEE641 pKa = 4.13EE642 pKa = 4.04ITEE645 pKa = 4.14PTADD649 pKa = 3.8LDD651 pKa = 3.84GDD653 pKa = 4.1FVVDD657 pKa = 5.01LGDD660 pKa = 3.19IALFAEE666 pKa = 5.57GWAMAEE672 pKa = 4.17YY673 pKa = 9.22MVSASGSEE681 pKa = 4.06PAGLRR686 pKa = 11.84AEE688 pKa = 4.2YY689 pKa = 10.31RR690 pKa = 11.84FEE692 pKa = 3.92EE693 pKa = 4.2LGGYY697 pKa = 8.59VVGDD701 pKa = 3.54SSGNGLDD708 pKa = 4.53GSIQRR713 pKa = 11.84DD714 pKa = 3.58LTGVWNVDD722 pKa = 3.44GFDD725 pKa = 3.91GGCVKK730 pKa = 9.96LTDD733 pKa = 4.07GSSVVLPASVFDD745 pKa = 4.24GVDD748 pKa = 3.3GFTMSFWMSQTTSAGVGMQAFTVDD772 pKa = 3.74SVGLDD777 pKa = 3.14ASYY780 pKa = 11.4EE781 pKa = 4.0LTNTGFYY788 pKa = 10.58DD789 pKa = 4.66GSWHH793 pKa = 7.11HH794 pKa = 6.2IAIVNDD800 pKa = 4.76AINARR805 pKa = 11.84VEE807 pKa = 4.28LYY809 pKa = 11.43VNGLLVAGGDD819 pKa = 3.4AGSTAEE825 pKa = 4.28AASEE829 pKa = 4.08TVIEE833 pKa = 4.95KK834 pKa = 10.45ISDD837 pKa = 3.46NAFGSTVDD845 pKa = 4.38KK846 pKa = 10.49IDD848 pKa = 3.55SVSVYY853 pKa = 10.67SRR855 pKa = 11.84ALDD858 pKa = 3.23HH859 pKa = 7.08SEE861 pKa = 3.46IVRR864 pKa = 11.84LYY866 pKa = 9.48GGAGASVMQPVNGSVTVSDD885 pKa = 4.2LNGDD889 pKa = 3.66GRR891 pKa = 11.84VDD893 pKa = 3.72LEE895 pKa = 5.44DD896 pKa = 3.56YY897 pKa = 11.45AEE899 pKa = 5.35LIGSMIQRR907 pKa = 11.84LL908 pKa = 3.59

Molecular weight: 94.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9NLP7|A0A1U9NLP7_9BACT Replication-associated recombination protein A OS=Anaerohalosphaera lusitana OX=1936003 GN=rarA PE=4 SV=1
MM1 pKa = 7.51PRR3 pKa = 11.84PVRR6 pKa = 11.84CRR8 pKa = 11.84RR9 pKa = 11.84VGMTPKK15 pKa = 9.47CTYY18 pKa = 9.83FKK20 pKa = 10.17PQGVRR25 pKa = 11.84LSALEE30 pKa = 4.13VVGLTVDD37 pKa = 3.13EE38 pKa = 4.83LEE40 pKa = 4.35AVRR43 pKa = 11.84LADD46 pKa = 5.51LEE48 pKa = 4.23GHH50 pKa = 5.42YY51 pKa = 8.23QQCAAEE57 pKa = 4.23KK58 pKa = 9.48MGVSRR63 pKa = 11.84QTFGRR68 pKa = 11.84IIEE71 pKa = 4.31SAHH74 pKa = 6.11KK75 pKa = 10.09KK76 pKa = 8.9IAEE79 pKa = 3.93ALVNGKK85 pKa = 9.28ALSIEE90 pKa = 4.21GGVVNLPEE98 pKa = 4.06EE99 pKa = 4.12ARR101 pKa = 11.84GRR103 pKa = 11.84GQGRR107 pKa = 11.84GRR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84GGGPGRR118 pKa = 11.84GRR120 pKa = 11.84CGQQ123 pKa = 3.26

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3413

0

3413

1238531

29

3724

362.9

40.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.512 ± 0.045

1.336 ± 0.017

6.493 ± 0.037

6.776 ± 0.049

3.945 ± 0.026

7.874 ± 0.047

1.931 ± 0.02

5.992 ± 0.036

5.544 ± 0.048

8.634 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.021

3.945 ± 0.029

4.196 ± 0.029

3.457 ± 0.028

5.258 ± 0.036

6.084 ± 0.037

5.473 ± 0.045

7.163 ± 0.035

1.462 ± 0.019

3.35 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski