Hubei lepidoptera virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Alphapaprhavirus; Hubei alphapaprhavirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMR9|A0A1L3KMR9_9VIRU Putative glycoprotein 1 OS=Hubei lepidoptera virus 2 OX=1922904 PE=4 SV=1
MM1 pKa = 6.71NTFRR5 pKa = 11.84RR6 pKa = 11.84INFDD10 pKa = 3.42KK11 pKa = 10.44EE12 pKa = 3.85KK13 pKa = 11.06KK14 pKa = 8.74EE15 pKa = 4.24ARR17 pKa = 11.84EE18 pKa = 3.87QNWNINLPKK27 pKa = 10.34AALEE31 pKa = 4.0QAQEE35 pKa = 4.07EE36 pKa = 4.89LGTDD40 pKa = 3.34SDD42 pKa = 4.63DD43 pKa = 3.82SAEE46 pKa = 4.16EE47 pKa = 4.02EE48 pKa = 5.04DD49 pKa = 3.91PGEE52 pKa = 5.32PKK54 pKa = 10.92DD55 pKa = 3.49PWNSVLTQNPVMSEE69 pKa = 4.09VNRR72 pKa = 11.84DD73 pKa = 3.46RR74 pKa = 11.84QFGSSDD80 pKa = 3.21IEE82 pKa = 3.96RR83 pKa = 11.84EE84 pKa = 4.28SAFSHH89 pKa = 6.75HH90 pKa = 6.38EE91 pKa = 4.25NIDD94 pKa = 3.78DD95 pKa = 3.85YY96 pKa = 11.82DD97 pKa = 4.39EE98 pKa = 4.77EE99 pKa = 4.47GHH101 pKa = 7.44AEE103 pKa = 4.24DD104 pKa = 6.15LIQSGDD110 pKa = 3.27VNQIVKK116 pKa = 10.36KK117 pKa = 10.37ALEE120 pKa = 4.26TSTPPVKK127 pKa = 10.46RR128 pKa = 11.84LGEE131 pKa = 3.89RR132 pKa = 11.84KK133 pKa = 9.33IQVNLQFMQSPPMMEE148 pKa = 3.69KK149 pKa = 9.62MKK151 pKa = 10.63DD152 pKa = 3.24IIHH155 pKa = 5.99EE156 pKa = 4.3VIKK159 pKa = 10.63AYY161 pKa = 6.79TTVPGRR167 pKa = 11.84VEE169 pKa = 4.25VSASDD174 pKa = 3.36DD175 pKa = 3.89GIYY178 pKa = 10.18IFNATNPYY186 pKa = 10.16LSPDD190 pKa = 3.62SDD192 pKa = 4.16SDD194 pKa = 3.91PLSSEE199 pKa = 3.88RR200 pKa = 11.84PYY202 pKa = 10.61PKK204 pKa = 9.86PKK206 pKa = 10.15EE207 pKa = 4.13KK208 pKa = 10.0PAPQQVQRR216 pKa = 11.84QFLPLEE222 pKa = 4.77ANTSTLTDD230 pKa = 3.39VEE232 pKa = 4.17IMIKK236 pKa = 10.3KK237 pKa = 9.73GVNFQGKK244 pKa = 9.28YY245 pKa = 9.52PGLPKK250 pKa = 10.55CIVRR254 pKa = 11.84YY255 pKa = 9.3GRR257 pKa = 11.84WGITEE262 pKa = 3.84QSVQLALKK270 pKa = 9.02EE271 pKa = 4.02NPNLSVNEE279 pKa = 4.18TIRR282 pKa = 11.84YY283 pKa = 8.94CMRR286 pKa = 11.84QAGTLKK292 pKa = 10.51KK293 pKa = 10.6FSGLYY298 pKa = 10.24KK299 pKa = 10.57FF300 pKa = 4.98

Molecular weight: 34.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMN7|A0A1L3KMN7_9VIRU Uncharacterized protein OS=Hubei lepidoptera virus 2 OX=1922904 PE=4 SV=1
MM1 pKa = 6.95KK2 pKa = 10.22QFVIACVKK10 pKa = 10.31PEE12 pKa = 4.02PSRR15 pKa = 11.84NSQDD19 pKa = 4.32CINSNYY25 pKa = 8.25NTTTIMLKK33 pKa = 8.91KK34 pKa = 8.62TGQGSLSSSRR44 pKa = 11.84SADD47 pKa = 3.44NEE49 pKa = 3.97IATSTDD55 pKa = 2.78SSKK58 pKa = 10.86GRR60 pKa = 11.84KK61 pKa = 8.27PKK63 pKa = 10.44NSPFSWFSKK72 pKa = 10.41KK73 pKa = 7.77PTRR76 pKa = 11.84PKK78 pKa = 9.28NHH80 pKa = 6.48QISVVKK86 pKa = 9.92PSAPPEE92 pKa = 3.93PQLRR96 pKa = 11.84FLEE99 pKa = 5.12DD100 pKa = 3.53IPVDD104 pKa = 3.18WTRR107 pKa = 11.84RR108 pKa = 11.84PEE110 pKa = 4.36DD111 pKa = 4.03EE112 pKa = 4.05SHH114 pKa = 5.93QPSIEE119 pKa = 3.9VDD121 pKa = 3.84LVSPIAFYY129 pKa = 9.8VTASLTLNSRR139 pKa = 11.84IKK141 pKa = 10.16IGSVEE146 pKa = 4.12SLLSKK151 pKa = 11.03LEE153 pKa = 3.67MWIDD157 pKa = 3.75HH158 pKa = 5.98YY159 pKa = 11.49SGSAVMYY166 pKa = 8.97PYY168 pKa = 9.57YY169 pKa = 10.15TLIYY173 pKa = 9.54ILLGSKK179 pKa = 10.35LSNRR183 pKa = 11.84PSNSKK188 pKa = 9.37MFLYY192 pKa = 10.67SSNFSGVIKK201 pKa = 10.6FGYY204 pKa = 7.95PQRR207 pKa = 11.84QGKK210 pKa = 8.11PPSEE214 pKa = 4.05TLSVLGHH221 pKa = 6.32EE222 pKa = 5.15GRR224 pKa = 11.84DD225 pKa = 3.54SNYY228 pKa = 9.9VSIRR232 pKa = 11.84FDD234 pKa = 3.07ATMKK238 pKa = 9.93EE239 pKa = 4.45SNRR242 pKa = 11.84NGVIYY247 pKa = 10.46SQLYY251 pKa = 9.25RR252 pKa = 11.84LNGYY256 pKa = 8.07NFPFPAALDD265 pKa = 3.84TFNLNIGLTHH275 pKa = 7.05EE276 pKa = 4.49GLIGPSPP283 pKa = 3.49

Molecular weight: 31.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4293

283

2145

715.5

81.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.913 ± 0.572

1.77 ± 0.372

5.404 ± 0.266

6.243 ± 0.322

4.449 ± 0.429

5.777 ± 0.359

2.516 ± 0.192

7.431 ± 0.609

6.336 ± 0.435

10.04 ± 0.763

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.272

5.334 ± 0.397

5.031 ± 0.487

3.75 ± 0.373

5.101 ± 0.476

8.642 ± 1.046

5.87 ± 0.273

5.101 ± 0.285

1.537 ± 0.183

3.704 ± 0.433
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski