Sewage-associated circular DNA virus-25

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH84|A0A0B4UH84_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-25 OX=1592092 PE=4 SV=1
MM1 pKa = 7.42SSSSRR6 pKa = 11.84WVFTVNNPGAWRR18 pKa = 11.84PHH20 pKa = 6.1YY21 pKa = 10.46DD22 pKa = 3.07VTAMQFLVFQMEE34 pKa = 4.25QGASGTPHH42 pKa = 5.25IQGYY46 pKa = 8.29VRR48 pKa = 11.84FTHH51 pKa = 6.12PKK53 pKa = 9.03KK54 pKa = 10.92LEE56 pKa = 3.82AAKK59 pKa = 10.25RR60 pKa = 11.84ALVCEE65 pKa = 4.26EE66 pKa = 3.61AHH68 pKa = 6.33MEE70 pKa = 3.92IANGNEE76 pKa = 4.16GQCIDD81 pKa = 3.94YY82 pKa = 8.27CTKK85 pKa = 9.99EE86 pKa = 4.04DD87 pKa = 3.71TRR89 pKa = 11.84IGGPWVYY96 pKa = 9.41GTQKK100 pKa = 10.3RR101 pKa = 11.84DD102 pKa = 3.15AGRR105 pKa = 11.84KK106 pKa = 8.66GSRR109 pKa = 11.84SDD111 pKa = 3.5LQAVAEE117 pKa = 4.27AAQKK121 pKa = 10.28PGVSMRR127 pKa = 11.84QLARR131 pKa = 11.84EE132 pKa = 4.53FPAQVIKK139 pKa = 10.84YY140 pKa = 7.55SQQIEE145 pKa = 4.35RR146 pKa = 11.84YY147 pKa = 7.03MALVEE152 pKa = 4.31DD153 pKa = 4.46EE154 pKa = 4.63VPIEE158 pKa = 3.75RR159 pKa = 11.84DD160 pKa = 3.05IFVHH164 pKa = 6.17VIWGPTGTGKK174 pKa = 7.06THH176 pKa = 6.89RR177 pKa = 11.84VRR179 pKa = 11.84HH180 pKa = 6.33GIPQRR185 pKa = 11.84DD186 pKa = 3.67LYY188 pKa = 10.71VVNGKK193 pKa = 9.52GRR195 pKa = 11.84GCFDD199 pKa = 3.06QYY201 pKa = 11.62AGQKK205 pKa = 10.62ALVLEE210 pKa = 4.31EE211 pKa = 4.55FEE213 pKa = 4.52PNEE216 pKa = 3.6WSINEE221 pKa = 4.16VKK223 pKa = 10.01TLLDD227 pKa = 3.16KK228 pKa = 7.62WTCPLNARR236 pKa = 11.84YY237 pKa = 9.95SNKK240 pKa = 7.53TARR243 pKa = 11.84WTQVFITSNSDD254 pKa = 2.95PMSWYY259 pKa = 10.16QLWLPADD266 pKa = 3.92LAAFQRR272 pKa = 11.84RR273 pKa = 11.84LTRR276 pKa = 11.84ITHH279 pKa = 4.65VLSRR283 pKa = 11.84EE284 pKa = 4.03QEE286 pKa = 4.14VEE288 pKa = 4.37LFPLPAVALPPPSPAVAPSPIAARR312 pKa = 11.84IAARR316 pKa = 11.84PCWSGAMDD324 pKa = 5.29ADD326 pKa = 5.0LPQPDD331 pKa = 4.38QVTSADD337 pKa = 3.76PLRR340 pKa = 11.84AAGTSNAPAIPTPRR354 pKa = 11.84LPTTPTPDD362 pKa = 3.77PLNVQDD368 pKa = 4.25IDD370 pKa = 4.33GIGAITAPAPVTRR383 pKa = 11.84TTSVDD388 pKa = 3.35LQAFFSNNN396 pKa = 2.84

Molecular weight: 43.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGY5|A0A0B4UGY5_9VIRU Uncharacterized protein OS=Sewage-associated circular DNA virus-25 OX=1592092 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84YY4 pKa = 8.62KK5 pKa = 10.55RR6 pKa = 11.84PSILSRR12 pKa = 11.84LKK14 pKa = 7.96TTKK17 pKa = 9.19KK18 pKa = 4.95TTRR21 pKa = 11.84GKK23 pKa = 8.0RR24 pKa = 11.84TKK26 pKa = 10.46YY27 pKa = 10.31RR28 pKa = 11.84LGSLGGAPKK37 pKa = 10.8AEE39 pKa = 4.02VKK41 pKa = 11.01SFDD44 pKa = 3.62TYY46 pKa = 11.45VLSNATGLPHH56 pKa = 7.03EE57 pKa = 5.35DD58 pKa = 2.88SAGFAEE64 pKa = 4.56PTSNFAGYY72 pKa = 7.93TCLNEE77 pKa = 4.31VSQGAAFYY85 pKa = 11.15ARR87 pKa = 11.84IGSKK91 pKa = 10.14ISIKK95 pKa = 10.35SIQVDD100 pKa = 4.34FDD102 pKa = 5.12LYY104 pKa = 10.06TLSITTTAGTARR116 pKa = 11.84CILIYY121 pKa = 10.56DD122 pKa = 4.11RR123 pKa = 11.84QTNGAAPSFVDD134 pKa = 3.78MFNAYY139 pKa = 8.4PAAINAFGTGINMTNRR155 pKa = 11.84SRR157 pKa = 11.84FSIIRR162 pKa = 11.84DD163 pKa = 3.15KK164 pKa = 11.26YY165 pKa = 9.12YY166 pKa = 10.56EE167 pKa = 4.28FGPGEE172 pKa = 4.26STTKK176 pKa = 9.95HH177 pKa = 3.55VHH179 pKa = 5.48MYY181 pKa = 11.13AKK183 pKa = 10.3GRR185 pKa = 11.84WDD187 pKa = 3.51TEE189 pKa = 4.63YY190 pKa = 11.12KK191 pKa = 10.67SSTPSVGDD199 pKa = 3.18ITTGAIYY206 pKa = 10.41LSVISNISLAYY217 pKa = 10.14SPGKK221 pKa = 9.83ICMNEE226 pKa = 2.89ITARR230 pKa = 11.84IRR232 pKa = 11.84YY233 pKa = 9.2LDD235 pKa = 3.32

Molecular weight: 25.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

916

235

396

305.3

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.515 ± 1.55

1.201 ± 0.219

4.803 ± 0.189

4.803 ± 0.476

4.148 ± 0.528

5.459 ± 1.325

1.856 ± 0.199

7.205 ± 1.366

4.913 ± 0.738

6.987 ± 0.906

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.528 ± 0.493

4.694 ± 0.851

6.769 ± 1.193

3.603 ± 1.18

6.114 ± 0.518

7.424 ± 1.036

7.969 ± 0.795

5.786 ± 0.856

1.419 ± 0.586

4.803 ± 1.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski