Escherichia phage PGT2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Ermolevavirus; Escherichia virus PGT2

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W2S4|A0A2D2W2S4_9CAUD Uncharacterized protein OS=Escherichia phage PGT2 OX=2047782 GN=PGT2_g00026 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.12IINPLHH8 pKa = 6.87LIQFVVVYY16 pKa = 9.67LYY18 pKa = 9.25TWYY21 pKa = 10.76ALRR24 pKa = 11.84SAPRR28 pKa = 11.84VEE30 pKa = 4.21GFNVGWSSPHH40 pKa = 7.62DD41 pKa = 4.22DD42 pKa = 3.43FQVYY46 pKa = 10.17YY47 pKa = 9.75NAQVNVLGVITPAQDD62 pKa = 3.51TTWQPVGWRR71 pKa = 11.84AWFNLVRR78 pKa = 11.84AYY80 pKa = 10.8DD81 pKa = 4.01DD82 pKa = 4.97LLEE85 pKa = 4.12QTTAAFIEE93 pKa = 5.0AYY95 pKa = 10.57LPDD98 pKa = 4.22FADD101 pKa = 6.12DD102 pKa = 3.69INEE105 pKa = 4.22SDD107 pKa = 4.36TEE109 pKa = 4.27DD110 pKa = 3.43KK111 pKa = 11.35

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W2Y6|A0A2D2W2Y6_9CAUD Uncharacterized protein OS=Escherichia phage PGT2 OX=2047782 GN=PGT2_g00001 PE=4 SV=1
MM1 pKa = 7.47NKK3 pKa = 9.68FKK5 pKa = 10.84QYY7 pKa = 11.06LGAAWDD13 pKa = 3.89FTKK16 pKa = 10.71EE17 pKa = 3.83HH18 pKa = 6.7GVTILRR24 pKa = 11.84GVAVLLVGRR33 pKa = 11.84KK34 pKa = 8.14VGRR37 pKa = 11.84VANQSADD44 pKa = 3.51VLDD47 pKa = 4.08TVIKK51 pKa = 9.29GTKK54 pKa = 9.56KK55 pKa = 10.38NN56 pKa = 3.52

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12884

37

1400

280.1

30.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.556 ± 0.738

0.97 ± 0.217

6.046 ± 0.25

5.782 ± 0.358

3.322 ± 0.18

7.381 ± 0.375

1.808 ± 0.224

4.898 ± 0.2

5.394 ± 0.415

7.769 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.236

4.626 ± 0.303

3.873 ± 0.29

4.804 ± 0.464

5.317 ± 0.276

6.434 ± 0.353

6.613 ± 0.388

6.683 ± 0.279

1.374 ± 0.108

3.57 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski