Bacillus phage pW4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R7R6|A0A3Q9R7R6_9CAUD RecB family exonuclease OS=Bacillus phage pW4 OX=2500560 GN=pW4_62 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 5.17YY3 pKa = 10.46IKK5 pKa = 10.8CSEE8 pKa = 4.33KK9 pKa = 10.73EE10 pKa = 3.9LTALEE15 pKa = 4.58TIVNALEE22 pKa = 4.32SFHH25 pKa = 6.95NVSTDD30 pKa = 3.33EE31 pKa = 3.9NDD33 pKa = 3.25INHH36 pKa = 6.86DD37 pKa = 3.78LEE39 pKa = 4.62VNNEE43 pKa = 3.71HH44 pKa = 7.29DD45 pKa = 4.81LNGLAYY51 pKa = 10.07DD52 pKa = 4.48IKK54 pKa = 11.48NNDD57 pKa = 3.62LFEE60 pKa = 4.41NWVKK64 pKa = 10.48FNKK67 pKa = 10.63GEE69 pKa = 4.37TKK71 pKa = 9.18VTAYY75 pKa = 10.62DD76 pKa = 3.55AVNGTGAQIIEE87 pKa = 4.29ILHH90 pKa = 5.73NNYY93 pKa = 10.78DD94 pKa = 3.64KK95 pKa = 11.57VFGYY99 pKa = 10.41YY100 pKa = 10.17EE101 pKa = 4.07EE102 pKa = 5.92DD103 pKa = 3.5GEE105 pKa = 4.8KK106 pKa = 10.17IFFVSEE112 pKa = 3.8QKK114 pKa = 11.0DD115 pKa = 3.58NYY117 pKa = 10.73NDD119 pKa = 3.32EE120 pKa = 4.45TGNDD124 pKa = 3.15EE125 pKa = 4.44PYY127 pKa = 10.6FFVGNTPVGMHH138 pKa = 6.02EE139 pKa = 4.4CLRR142 pKa = 11.84VQQ144 pKa = 3.86

Molecular weight: 16.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IHX0|A0A3T0IHX0_9CAUD Uncharacterized protein OS=Bacillus phage pW4 OX=2500560 GN=pW4_10 PE=4 SV=1
MM1 pKa = 7.79NIRR4 pKa = 11.84FQRR7 pKa = 11.84KK8 pKa = 8.61DD9 pKa = 3.26RR10 pKa = 11.84NKK12 pKa = 10.56QLMTKK17 pKa = 10.2ANSKK21 pKa = 9.86FEE23 pKa = 4.4RR24 pKa = 11.84KK25 pKa = 9.53VKK27 pKa = 10.05EE28 pKa = 3.81AFITKK33 pKa = 7.83QLKK36 pKa = 10.08KK37 pKa = 10.0RR38 pKa = 11.84GKK40 pKa = 9.71NNEE43 pKa = 3.87SYY45 pKa = 10.95KK46 pKa = 10.58RR47 pKa = 3.61

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

22317

47

2250

210.5

23.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.094 ± 0.463

0.789 ± 0.109

6.452 ± 0.188

7.532 ± 0.413

4.463 ± 0.197

6.538 ± 0.352

1.658 ± 0.156

6.815 ± 0.281

9.042 ± 0.405

7.6 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.989 ± 0.189

6.224 ± 0.314

2.689 ± 0.198

3.836 ± 0.365

3.8 ± 0.17

5.399 ± 0.343

6.0 ± 0.372

6.506 ± 0.227

1.187 ± 0.112

4.387 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski