Tarumizu tick virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Coltivirus; Tarumizu coltivirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A292G307|A0A292G307_9REOV VP5 OS=Tarumizu tick virus OX=2014339 GN=VP5 PE=4 SV=1
MM1 pKa = 7.87GDD3 pKa = 3.14IQTEE7 pKa = 4.2GLNISGSIQAVVQTNDD23 pKa = 3.19GAHH26 pKa = 6.24FMCLIRR32 pKa = 11.84GRR34 pKa = 11.84HH35 pKa = 6.27CINISDD41 pKa = 4.21LWNYY45 pKa = 9.57SLPEE49 pKa = 3.72GWIFGALNHH58 pKa = 6.7FYY60 pKa = 10.83CFVPADD66 pKa = 3.35IGVIEE71 pKa = 4.89CEE73 pKa = 4.27FFRR76 pKa = 11.84QRR78 pKa = 11.84GVEE81 pKa = 4.11VVPVGNVDD89 pKa = 5.71EE90 pKa = 4.31FLQWYY95 pKa = 9.53LNLGDD100 pKa = 3.82AVRR103 pKa = 11.84VVHH106 pKa = 6.96HH107 pKa = 5.52YY108 pKa = 9.83VAYY111 pKa = 10.75LGAPCVGQVEE121 pKa = 5.2AILEE125 pKa = 4.38LGSRR129 pKa = 11.84WQFIPRR135 pKa = 11.84PEE137 pKa = 4.05NYY139 pKa = 9.23VVRR142 pKa = 11.84TAAEE146 pKa = 3.87LKK148 pKa = 10.33DD149 pKa = 3.16AFRR152 pKa = 11.84RR153 pKa = 11.84VGANRR158 pKa = 11.84EE159 pKa = 4.16YY160 pKa = 11.36AEE162 pKa = 3.81GMYY165 pKa = 10.12RR166 pKa = 11.84DD167 pKa = 3.99GGADD171 pKa = 3.23RR172 pKa = 11.84LVHH175 pKa = 6.27RR176 pKa = 11.84AEE178 pKa = 4.42LNVGDD183 pKa = 4.48FAEE186 pKa = 4.97APAAVPEE193 pKa = 4.62APPAPALPPPPPAPADD209 pKa = 3.66GQLGEE214 pKa = 4.46DD215 pKa = 4.49HH216 pKa = 7.16IEE218 pKa = 4.11HH219 pKa = 6.27LQGLARR225 pKa = 11.84AQAEE229 pKa = 4.79GEE231 pKa = 4.33GQPPAPQPPRR241 pKa = 11.84QEE243 pKa = 4.18EE244 pKa = 4.34APPPAPAPAAVGPAPRR260 pKa = 11.84DD261 pKa = 3.53VQPFNLRR268 pKa = 11.84EE269 pKa = 3.86NLVRR273 pKa = 11.84LDD275 pKa = 3.54ALSARR280 pKa = 11.84FLDD283 pKa = 5.02AYY285 pKa = 9.96NQLGAQVGAFQDD297 pKa = 4.08GVITVDD303 pKa = 3.08NLPNFRR309 pKa = 11.84EE310 pKa = 4.09AFNLIEE316 pKa = 4.59GVVLEE321 pKa = 4.87CQAEE325 pKa = 4.05FDD327 pKa = 4.33PYY329 pKa = 10.59RR330 pKa = 11.84LPGRR334 pKa = 11.84HH335 pKa = 5.98YY336 pKa = 10.79NPQFADD342 pKa = 3.54DD343 pKa = 3.67ARR345 pKa = 11.84NNIEE349 pKa = 3.81AAMNAARR356 pKa = 11.84VRR358 pKa = 11.84HH359 pKa = 6.28HH360 pKa = 6.78GVQPVVPGALDD371 pKa = 3.5EE372 pKa = 4.77YY373 pKa = 9.45NNNMHH378 pKa = 5.75GVEE381 pKa = 3.92WATVNLRR388 pKa = 11.84VQSLNQRR395 pKa = 11.84LVDD398 pKa = 3.65CGFIVGLGGTLQQIVRR414 pKa = 11.84HH415 pKa = 5.69AGVNVVHH422 pKa = 7.01RR423 pKa = 11.84GAYY426 pKa = 8.33IDD428 pKa = 4.28PYY430 pKa = 9.47VEE432 pKa = 4.0NMQFSWHH439 pKa = 5.89GNGRR443 pKa = 11.84CVSASRR449 pKa = 11.84FFTQFFYY456 pKa = 10.9GMPDD460 pKa = 2.79LHH462 pKa = 7.44YY463 pKa = 10.95GLNEE467 pKa = 4.26LGRR470 pKa = 11.84VMRR473 pKa = 11.84DD474 pKa = 2.9LQTRR478 pKa = 11.84VSSYY482 pKa = 11.45DD483 pKa = 3.21SVSFDD488 pKa = 5.1RR489 pKa = 11.84IYY491 pKa = 11.05EE492 pKa = 4.26PNDD495 pKa = 3.46LLDD498 pKa = 4.27LNGMNEE504 pKa = 4.29LCRR507 pKa = 11.84SIFVMLRR514 pKa = 11.84RR515 pKa = 11.84WHH517 pKa = 5.7TLALRR522 pKa = 11.84LRR524 pKa = 11.84NPAHH528 pKa = 6.31HH529 pKa = 6.95RR530 pKa = 11.84NVCMEE535 pKa = 4.44LARR538 pKa = 11.84RR539 pKa = 11.84FAGWGPGVRR548 pKa = 11.84YY549 pKa = 7.41TIPAGVLRR557 pKa = 11.84VFRR560 pKa = 11.84TKK562 pKa = 10.74HH563 pKa = 6.2LINLIDD569 pKa = 4.34PGACYY574 pKa = 9.66TLLHH578 pKa = 6.23YY579 pKa = 10.61RR580 pKa = 11.84LEE582 pKa = 4.65HH583 pKa = 6.72PLGAVQNPRR592 pKa = 11.84EE593 pKa = 4.69CILDD597 pKa = 3.95LARR600 pKa = 11.84VGEE603 pKa = 4.21WANIHH608 pKa = 7.26DD609 pKa = 4.1ILPYY613 pKa = 9.29MVHH616 pKa = 6.55FEE618 pKa = 3.9FWEE621 pKa = 4.13MFVNWFPDD629 pKa = 3.06RR630 pKa = 11.84GLDD633 pKa = 3.59GVEE636 pKa = 3.87YY637 pKa = 9.89LAPVFPTYY645 pKa = 10.84LSDD648 pKa = 3.85RR649 pKa = 11.84EE650 pKa = 3.99LWTAVRR656 pKa = 11.84LLL658 pKa = 3.78

Molecular weight: 73.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A292G3A9|A0A292G3A9_9REOV VP8 OS=Tarumizu tick virus OX=2014339 GN=VP8 PE=4 SV=1
MM1 pKa = 7.61FILVSVGLVDD11 pKa = 4.5RR12 pKa = 11.84APQRR16 pKa = 11.84GYY18 pKa = 11.05NPSKK22 pKa = 10.56RR23 pKa = 11.84DD24 pKa = 3.5MLIMRR29 pKa = 11.84SIATHH34 pKa = 5.59YY35 pKa = 11.29GNDD38 pKa = 4.61KK39 pKa = 9.65MLLWFGDD46 pKa = 3.62YY47 pKa = 10.88HH48 pKa = 7.56DD49 pKa = 4.64ITVSHH54 pKa = 6.62AKK56 pKa = 9.84IVFGDD61 pKa = 3.67DD62 pKa = 2.78KK63 pKa = 11.27RR64 pKa = 11.84FAFLGTNATASGRR77 pKa = 11.84LTMPYY82 pKa = 9.51PRR84 pKa = 11.84ASSEE88 pKa = 3.86ISWRR92 pKa = 11.84EE93 pKa = 3.59VTSGYY98 pKa = 9.58LAKK101 pKa = 10.43EE102 pKa = 4.14EE103 pKa = 4.11KK104 pKa = 10.23EE105 pKa = 4.05KK106 pKa = 10.73IVVYY110 pKa = 10.26IHH112 pKa = 6.76EE113 pKa = 4.82PLNFVDD119 pKa = 5.12QSTKK123 pKa = 10.67KK124 pKa = 9.33PVPMDD129 pKa = 3.98EE130 pKa = 4.15VLKK133 pKa = 10.33WLATNVVGPIVIRR146 pKa = 11.84MDD148 pKa = 3.7CKK150 pKa = 10.1KK151 pKa = 9.96HH152 pKa = 5.84YY153 pKa = 10.49DD154 pKa = 3.47GLVPKK159 pKa = 10.81GNMYY163 pKa = 10.44YY164 pKa = 10.76LPFEE168 pKa = 4.73KK169 pKa = 10.31KK170 pKa = 10.32LSLRR174 pKa = 11.84YY175 pKa = 9.92LLVNDD180 pKa = 5.18CGRR183 pKa = 11.84SLDD186 pKa = 3.76WTVGASFDD194 pKa = 3.55VRR196 pKa = 11.84PYY198 pKa = 9.94LTGFNLITRR207 pKa = 11.84SSPVFSGSYY216 pKa = 9.74DD217 pKa = 3.43QAVYY221 pKa = 11.49AMMTQATVPRR231 pKa = 11.84STVDD235 pKa = 2.99EE236 pKa = 4.43RR237 pKa = 11.84EE238 pKa = 3.95LRR240 pKa = 11.84NVITEE245 pKa = 4.22LCYY248 pKa = 10.08EE249 pKa = 4.31HH250 pKa = 6.71QFPTPEE256 pKa = 3.78IHH258 pKa = 5.85STTARR263 pKa = 11.84RR264 pKa = 11.84SGQAGEE270 pKa = 4.13VWTTSIRR277 pKa = 11.84AGPLFTGQHH286 pKa = 5.37IADD289 pKa = 4.08TKK291 pKa = 10.89EE292 pKa = 3.68KK293 pKa = 10.76SLDD296 pKa = 3.26LAYY299 pKa = 10.77RR300 pKa = 11.84EE301 pKa = 3.94MLGILEE307 pKa = 4.25QLTVQPRR314 pKa = 11.84DD315 pKa = 3.56LGRR318 pKa = 11.84GCCXRR323 pKa = 11.84CWPGEE328 pKa = 3.83LFIVNVEE335 pKa = 4.24TEE337 pKa = 4.35SFRR340 pKa = 11.84SGCSRR345 pKa = 11.84EE346 pKa = 3.84AAYY349 pKa = 10.2QAGAFWSLLQWRR361 pKa = 11.84RR362 pKa = 11.84HH363 pKa = 5.56YY364 pKa = 10.54IVSFPDD370 pKa = 3.19LRR372 pKa = 11.84GAVCWLNEE380 pKa = 3.82VLRR383 pKa = 11.84LRR385 pKa = 11.84TRR387 pKa = 11.84RR388 pKa = 11.84SRR390 pKa = 11.84GRR392 pKa = 11.84AYY394 pKa = 9.83ILFGCHH400 pKa = 6.15SVNEE404 pKa = 4.18LMGDD408 pKa = 3.38SHH410 pKa = 7.24GGSFTVADD418 pKa = 3.6IRR420 pKa = 11.84EE421 pKa = 4.2SLNFGVLQGSFEE433 pKa = 4.26DD434 pKa = 6.08AVFIWQRR441 pKa = 11.84CRR443 pKa = 11.84VGRR446 pKa = 11.84LFDD449 pKa = 3.55VFGSRR454 pKa = 11.84FFSPVSEE461 pKa = 4.08DD462 pKa = 3.09VKK464 pKa = 10.89RR465 pKa = 11.84FCQEE469 pKa = 2.89MRR471 pKa = 11.84QQEE474 pKa = 4.33TRR476 pKa = 11.84SDD478 pKa = 3.13WFDD481 pKa = 3.87WILKK485 pKa = 8.52SCLRR489 pKa = 11.84VHH491 pKa = 6.71HH492 pKa = 6.61EE493 pKa = 3.94IGLSFNLGSFLGRR506 pKa = 11.84ATYY509 pKa = 10.1HH510 pKa = 6.35FEE512 pKa = 3.75VASYY516 pKa = 11.55GEE518 pKa = 4.22TTTRR522 pKa = 11.84EE523 pKa = 3.87LRR525 pKa = 11.84GARR528 pKa = 11.84STRR531 pKa = 11.84PAIKK535 pKa = 10.32VV536 pKa = 3.3

Molecular weight: 61.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

9520

209

1433

732.3

82.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.565 ± 0.437

1.828 ± 0.116

5.977 ± 0.258

5.819 ± 0.265

4.496 ± 0.231

7.521 ± 0.183

2.489 ± 0.231

5.021 ± 0.255

3.992 ± 0.38

9.38 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.189

3.855 ± 0.304

4.664 ± 0.424

3.277 ± 0.229

7.342 ± 0.435

6.775 ± 0.566

4.517 ± 0.325

8.487 ± 0.201

1.597 ± 0.153

3.592 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski