Enterococcus casseliflavus EC20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus casseliflavus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9A8G2|C9A8G2_ENTCA Uncharacterized protein OS=Enterococcus casseliflavus EC20 OX=565655 GN=ECBG_01042 PE=4 SV=2
MM1 pKa = 7.76YY2 pKa = 9.86YY3 pKa = 9.94QQALQPNEE11 pKa = 4.16LLPAISNSGEE21 pKa = 3.98CFFVIRR27 pKa = 11.84AEE29 pKa = 4.09LPIRR33 pKa = 11.84QYY35 pKa = 11.1QIAVYY40 pKa = 10.05LYY42 pKa = 10.02DD43 pKa = 4.02DD44 pKa = 4.52QFFLLQDD51 pKa = 3.88DD52 pKa = 4.59RR53 pKa = 11.84LFDD56 pKa = 4.17QIDD59 pKa = 4.2HH60 pKa = 6.85ISSEE64 pKa = 4.31TLGDD68 pKa = 3.68EE69 pKa = 4.58EE70 pKa = 5.16EE71 pKa = 4.02ILPFIEE77 pKa = 4.39EE78 pKa = 4.06ALEE81 pKa = 4.07EE82 pKa = 3.98NHH84 pKa = 6.33YY85 pKa = 11.27LLVEE89 pKa = 4.01KK90 pKa = 10.93AFIRR94 pKa = 11.84LDD96 pKa = 3.46LSTLQKK102 pKa = 8.55MTDD105 pKa = 3.35LTSFDD110 pKa = 3.03ILFYY114 pKa = 11.09EE115 pKa = 5.64FFDD118 pKa = 3.55SWGEE122 pKa = 3.91EE123 pKa = 4.3GG124 pKa = 5.0

Molecular weight: 14.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9A8R9|C9A8R9_ENTCA Beta sliding clamp OS=Enterococcus casseliflavus EC20 OX=565655 GN=ECBG_01149 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.33GRR39 pKa = 11.84KK40 pKa = 9.06VISAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3112

0

3112

993929

29

3130

319.4

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.148 ± 0.045

0.622 ± 0.012

5.153 ± 0.038

7.323 ± 0.05

4.714 ± 0.035

6.604 ± 0.046

1.822 ± 0.019

7.24 ± 0.037

5.887 ± 0.043

10.067 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.023

4.133 ± 0.036

3.703 ± 0.026

4.731 ± 0.041

3.933 ± 0.032

5.831 ± 0.035

6.089 ± 0.046

6.841 ± 0.039

0.993 ± 0.015

3.592 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski