Novosphingobium sp. PC22D

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A8HUX8|A0A2A8HUX8_9SPHN Zn-dependent hydrolase OS=Novosphingobium sp. PC22D OX=1962403 GN=B2G71_10410 PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 10.25NIHH5 pKa = 6.28GLLLVGCSALALAGCGPNEE24 pKa = 4.05IASPGTGGNITVNNPTPAPTPTPTPTPTPTSSLVTPAAGCPTIAASGGLGNEE76 pKa = 4.4GTISGPTGEE85 pKa = 4.58YY86 pKa = 9.52RR87 pKa = 11.84VCTLPTTFDD96 pKa = 3.35ASSTLPYY103 pKa = 10.13IEE105 pKa = 4.32GVLYY109 pKa = 10.84RR110 pKa = 11.84MNGRR114 pKa = 11.84VDD116 pKa = 3.37VGSDD120 pKa = 3.52GGPTPDD126 pKa = 5.27DD127 pKa = 3.81SDD129 pKa = 3.88GLSDD133 pKa = 3.75TNVTLKK139 pKa = 10.33IEE141 pKa = 4.41PGVIIYY147 pKa = 10.69SSGSSFLYY155 pKa = 10.3VNRR158 pKa = 11.84GNKK161 pKa = 8.7IDD163 pKa = 3.86AVGTKK168 pKa = 9.16MMPIVFTSRR177 pKa = 11.84DD178 pKa = 3.46NVQGLNNNNSSGQWGGVVLGGRR200 pKa = 11.84AQVTDD205 pKa = 3.85CDD207 pKa = 3.96APNAAEE213 pKa = 4.12GTIEE217 pKa = 5.07CEE219 pKa = 3.98RR220 pKa = 11.84QVEE223 pKa = 4.99GSATPAFFGGATNDD237 pKa = 3.79DD238 pKa = 3.98DD239 pKa = 5.09SGVMKK244 pKa = 10.15YY245 pKa = 8.5VQIRR249 pKa = 11.84YY250 pKa = 9.99SGFTLTGGSEE260 pKa = 4.19LQSLTTGGTGTGTEE274 pKa = 3.79FDD276 pKa = 4.7YY277 pKa = 11.2IQSFNSSDD285 pKa = 3.46DD286 pKa = 3.45GAEE289 pKa = 3.92FFGGYY294 pKa = 10.14VNMKK298 pKa = 9.69HH299 pKa = 6.37FIVVGAEE306 pKa = 3.95DD307 pKa = 4.94DD308 pKa = 5.35SIDD311 pKa = 3.71SDD313 pKa = 3.8TGVKK317 pKa = 10.66ANIQYY322 pKa = 10.75AIVVQDD328 pKa = 3.31SDD330 pKa = 3.82RR331 pKa = 11.84AHH333 pKa = 6.99DD334 pKa = 5.07AIIEE338 pKa = 4.18ADD340 pKa = 3.7SNDD343 pKa = 3.15NRR345 pKa = 11.84NAVPRR350 pKa = 11.84QDD352 pKa = 3.26TKK354 pKa = 10.66ISNFVFISPTAPADD368 pKa = 3.38AGAAVYY374 pKa = 9.86FRR376 pKa = 11.84GGTDD380 pKa = 3.27YY381 pKa = 11.87ALLNGIILSPNDD393 pKa = 2.8EE394 pKa = 4.95CIRR397 pKa = 11.84VRR399 pKa = 11.84HH400 pKa = 6.08AEE402 pKa = 4.17TVQASGDD409 pKa = 3.99DD410 pKa = 3.91EE411 pKa = 4.79NGPPVFEE418 pKa = 4.67SVQLSCSSAGQFVGSDD434 pKa = 3.36GPTADD439 pKa = 4.27DD440 pKa = 3.4VAGFFNAGSNNNATYY455 pKa = 10.34TSTLTNLFINGSNEE469 pKa = 3.83GGVTAFDD476 pKa = 3.98PTTLSSFFDD485 pKa = 3.33AVDD488 pKa = 3.51YY489 pKa = 11.16IGAVKK494 pKa = 10.32NAADD498 pKa = 3.15TWYY501 pKa = 9.54TGWTCNSDD509 pKa = 2.99IADD512 pKa = 4.17FGDD515 pKa = 3.44NTGACTSLPVYY526 pKa = 10.57

Molecular weight: 54.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A8I046|A0A2A8I046_9SPHN HTH luxR-type domain-containing protein OS=Novosphingobium sp. PC22D OX=1962403 GN=B2G71_00260 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.57GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.1ATPGGRR28 pKa = 11.84KK29 pKa = 8.0VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4593

0

4593

1478106

25

2582

321.8

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.086 ± 0.053

0.901 ± 0.013

6.062 ± 0.033

6.132 ± 0.031

3.618 ± 0.021

8.821 ± 0.034

1.996 ± 0.018

4.852 ± 0.023

2.89 ± 0.031

9.849 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.022

2.402 ± 0.023

5.241 ± 0.024

2.933 ± 0.018

7.5 ± 0.044

5.35 ± 0.031

5.064 ± 0.029

7.135 ± 0.027

1.47 ± 0.015

2.248 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski