Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Bordetella; Bordetella pertussis

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7VXJ9|Q7VXJ9_BORPE Probable fatty acid desaturase OS=Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) OX=257313 GN=BP1758 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.89ISSHH6 pKa = 6.33LLAEE10 pKa = 4.62LANGVCLEE18 pKa = 4.22TRR20 pKa = 11.84VDD22 pKa = 3.48GQTIGTYY29 pKa = 10.18VVLGPADD36 pKa = 4.2LDD38 pKa = 4.45AIADD42 pKa = 3.48IVPRR46 pKa = 11.84QRR48 pKa = 11.84VEE50 pKa = 3.94EE51 pKa = 4.38GADD54 pKa = 2.93IHH56 pKa = 6.58AAAVDD61 pKa = 4.29SVDD64 pKa = 3.56GAQMEE69 pKa = 4.07IDD71 pKa = 3.35QVIEE75 pKa = 3.91NMNPGDD81 pKa = 3.62VAVFFCADD89 pKa = 3.78GDD91 pKa = 4.5CYY93 pKa = 11.06GAALDD98 pKa = 4.89LLGLPVDD105 pKa = 4.09EE106 pKa = 5.09

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7VSH7|Q7VSH7_BORPE Acetyl-CoA acetyltransferase OS=Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) OX=257313 GN=BP0447 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.24TRR25 pKa = 11.84AGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3258

0

3258

1063353

37

4196

326.4

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.641 ± 0.067

0.91 ± 0.013

5.368 ± 0.031

5.003 ± 0.042

3.274 ± 0.03

8.639 ± 0.047

2.229 ± 0.02

4.383 ± 0.033

2.688 ± 0.036

10.791 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.021

2.452 ± 0.026

5.38 ± 0.031

4.063 ± 0.027

7.486 ± 0.042

4.886 ± 0.03

4.836 ± 0.03

7.655 ± 0.037

1.415 ± 0.018

2.446 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski