Necator americanus (Human hookworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Strongylida; Ancylostomatoidea; Ancylostomatidae; Bunostominae; Necator

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W2TP56|W2TP56_NECAM Glycylpeptide N-tetradecanoyltransferase OS=Necator americanus OX=51031 GN=NECAME_07162 PE=3 SV=1
MM1 pKa = 7.53LLYY4 pKa = 10.94LFGLFLILPFSLAEE18 pKa = 4.25DD19 pKa = 3.84TDD21 pKa = 4.85LEE23 pKa = 5.36DD24 pKa = 4.49DD25 pKa = 4.34LEE27 pKa = 5.61GGSEE31 pKa = 4.36EE32 pKa = 4.41DD33 pKa = 4.46TNDD36 pKa = 3.24TGDD39 pKa = 3.7NNEE42 pKa = 4.19EE43 pKa = 4.08TDD45 pKa = 3.82SGSSGDD51 pKa = 4.79DD52 pKa = 3.09SDD54 pKa = 5.5SSDD57 pKa = 3.68EE58 pKa = 4.49GGCADD63 pKa = 3.59AAKK66 pKa = 10.3YY67 pKa = 10.51DD68 pKa = 3.91KK69 pKa = 10.92LCPYY73 pKa = 10.33LKK75 pKa = 10.64EE76 pKa = 4.34KK77 pKa = 10.51GFCSGYY83 pKa = 10.51LQDD86 pKa = 6.24HH87 pKa = 5.46MKK89 pKa = 9.97KK90 pKa = 8.81WCKK93 pKa = 8.5KK94 pKa = 7.77TCGSCC99 pKa = 3.67

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W2TVP1|W2TVP1_NECAM Uncharacterized protein OS=Necator americanus OX=51031 GN=NECAME_06237 PE=4 SV=1
MM1 pKa = 7.43TLRR4 pKa = 11.84AALRR8 pKa = 11.84KK9 pKa = 7.94TPTILVITMRR19 pKa = 11.84RR20 pKa = 11.84QTVVLQATTVTRR32 pKa = 11.84VMKK35 pKa = 10.57AVALTQRR42 pKa = 11.84SMTNSARR49 pKa = 11.84TT50 pKa = 3.39

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19125

0

19125

5133344

26

5238

268.4

30.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.064 ± 0.016

2.154 ± 0.017

5.421 ± 0.017

6.663 ± 0.024

4.171 ± 0.015

5.836 ± 0.026

2.416 ± 0.01

5.398 ± 0.015

5.739 ± 0.022

8.914 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.01

4.167 ± 0.013

5.14 ± 0.028

3.864 ± 0.014

6.227 ± 0.02

7.771 ± 0.026

5.641 ± 0.019

6.652 ± 0.017

1.126 ± 0.006

3.021 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski