Amino acid dipepetide frequency for Necator americanus (Human hookworm)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.554AlaAla: 6.554 ± 0.054	1.325AlaCys: 1.325 ± 0.017	3.565AlaAsp: 3.565 ± 0.027	4.653AlaGlu: 4.653 ± 0.037	2.847AlaPhe: 2.847 ± 0.023	3.975AlaGly: 3.975 ± 0.034	1.62AlaHis: 1.62 ± 0.018	3.908AlaIle: 3.908 ± 0.029	4.022AlaLys: 4.022 ± 0.031	6.463AlaLeu: 6.463 ± 0.037	1.834AlaMet: 1.834 ± 0.02	2.717AlaAsn: 2.717 ± 0.024	3.617AlaPro: 3.617 ± 0.046	2.704AlaGln: 2.704 ± 0.027	4.023AlaArg: 4.023 ± 0.035	5.083AlaSer: 5.083 ± 0.033	3.957AlaThr: 3.957 ± 0.033	5.225AlaVal: 5.225 ± 0.033	0.667AlaTrp: 0.667 ± 0.012	1.884AlaTyr: 1.884 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	1.484CysAla: 1.484 ± 0.019	0.596CysCys: 0.596 ± 0.014	1.255CysAsp: 1.255 ± 0.02	1.337CysGlu: 1.337 ± 0.019	0.886CysPhe: 0.886 ± 0.012	1.526CysGly: 1.526 ± 0.022	0.527CysHis: 0.527 ± 0.011	1.121CysIle: 1.121 ± 0.018	1.032CysLys: 1.032 ± 0.016	1.778CysLeu: 1.778 ± 0.019	0.519CysMet: 0.519 ± 0.011	0.872CysAsn: 0.872 ± 0.015	1.228CysPro: 1.228 ± 0.027	0.774CysGln: 0.774 ± 0.016	1.323CysArg: 1.323 ± 0.018	1.83CysSer: 1.83 ± 0.022	1.168CysThr: 1.168 ± 0.016	1.458CysVal: 1.458 ± 0.022	0.223CysTrp: 0.223 ± 0.007	0.604CysTyr: 0.604 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.658AspAla: 3.658 ± 0.027	1.1AspCys: 1.1 ± 0.021	3.851AspAsp: 3.851 ± 0.035	4.381AspGlu: 4.381 ± 0.036	2.319AspPhe: 2.319 ± 0.023	3.577AspGly: 3.577 ± 0.035	1.286AspHis: 1.286 ± 0.017	2.966AspIle: 2.966 ± 0.025	2.642AspLys: 2.642 ± 0.025	4.729AspLeu: 4.729 ± 0.033	1.324AspMet: 1.324 ± 0.016	1.948AspAsn: 1.948 ± 0.019	2.55AspPro: 2.55 ± 0.024	1.854AspGln: 1.854 ± 0.019	3.147AspArg: 3.147 ± 0.032	3.948AspSer: 3.948 ± 0.035	2.483AspThr: 2.483 ± 0.024	4.062AspVal: 4.062 ± 0.029	0.655AspTrp: 0.655 ± 0.012	1.73AspTyr: 1.73 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	4.516GluAla: 4.516 ± 0.037	1.273GluCys: 1.273 ± 0.022	3.889GluAsp: 3.889 ± 0.033	6.225GluGlu: 6.225 ± 0.062	2.346GluPhe: 2.346 ± 0.021	3.192GluGly: 3.192 ± 0.027	1.587GluHis: 1.587 ± 0.019	3.549GluIle: 3.549 ± 0.026	5.198GluLys: 5.198 ± 0.048	5.744GluLeu: 5.744 ± 0.047	1.925GluMet: 1.925 ± 0.019	3.178GluAsn: 3.178 ± 0.023	2.446GluPro: 2.446 ± 0.029	2.878GluGln: 2.878 ± 0.028	4.404GluArg: 4.404 ± 0.033	4.221GluSer: 4.221 ± 0.031	3.446GluThr: 3.446 ± 0.028	3.976GluVal: 3.976 ± 0.032	0.785GluTrp: 0.785 ± 0.012	1.855GluTyr: 1.855 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	2.933PheAla: 2.933 ± 0.027	1.05PheCys: 1.05 ± 0.016	2.414PheAsp: 2.414 ± 0.018	2.479PheGlu: 2.479 ± 0.023	2.088PhePhe: 2.088 ± 0.023	2.699PheGly: 2.699 ± 0.025	1.022PheHis: 1.022 ± 0.015	2.275PheIle: 2.275 ± 0.021	1.843PheLys: 1.843 ± 0.02	3.982PheLeu: 3.982 ± 0.033	0.994PheMet: 0.994 ± 0.015	1.686PheAsn: 1.686 ± 0.018	1.912PhePro: 1.912 ± 0.023	1.409PheGln: 1.409 ± 0.017	2.279PheArg: 2.279 ± 0.023	3.273PheSer: 3.273 ± 0.028	2.292PheThr: 2.292 ± 0.024	3.045PheVal: 3.045 ± 0.026	0.502PheTrp: 0.502 ± 0.01	1.535PheTyr: 1.535 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	3.899GlyAla: 3.899 ± 0.035	1.28GlyCys: 1.28 ± 0.02	3.122GlyAsp: 3.122 ± 0.028	3.625GlyGlu: 3.625 ± 0.033	2.566GlyPhe: 2.566 ± 0.026	4.481GlyGly: 4.481 ± 0.069	1.351GlyHis: 1.351 ± 0.02	3.077GlyIle: 3.077 ± 0.029	3.416GlyLys: 3.416 ± 0.031	4.523GlyLeu: 4.523 ± 0.039	1.525GlyMet: 1.525 ± 0.024	2.465GlyAsn: 2.465 ± 0.024	2.785GlyPro: 2.785 ± 0.052	2.147GlyGln: 2.147 ± 0.03	3.603GlyArg: 3.603 ± 0.035	4.51GlySer: 4.51 ± 0.036	3.315GlyThr: 3.315 ± 0.033	3.956GlyVal: 3.956 ± 0.034	0.709GlyTrp: 0.709 ± 0.013	2.01GlyTyr: 2.01 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	1.515HisAla: 1.515 ± 0.017	0.578HisCys: 0.578 ± 0.012	1.211HisAsp: 1.211 ± 0.015	1.428HisGlu: 1.428 ± 0.016	1.103HisPhe: 1.103 ± 0.015	1.464HisGly: 1.464 ± 0.017	0.779HisHis: 0.779 ± 0.014	1.373HisIle: 1.373 ± 0.016	1.117HisLys: 1.117 ± 0.015	2.321HisLeu: 2.321 ± 0.022	0.613HisMet: 0.613 ± 0.011	0.93HisAsn: 0.93 ± 0.012	1.38HisPro: 1.38 ± 0.02	0.916HisGln: 0.916 ± 0.013	1.635HisArg: 1.635 ± 0.019	1.854HisSer: 1.854 ± 0.02	1.219HisThr: 1.219 ± 0.016	1.601HisVal: 1.601 ± 0.016	0.304HisTrp: 0.304 ± 0.008	0.822HisTyr: 0.822 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.046IleAla: 4.046 ± 0.029	1.326IleCys: 1.326 ± 0.019	3.17IleAsp: 3.17 ± 0.026	3.419IleGlu: 3.419 ± 0.029	2.381IlePhe: 2.381 ± 0.029	3.278IleGly: 3.278 ± 0.028	1.368IleHis: 1.368 ± 0.017	2.866IleIle: 2.866 ± 0.029	2.633IleLys: 2.633 ± 0.026	4.682IleLeu: 4.682 ± 0.04	1.281IleMet: 1.281 ± 0.016	2.127IleAsn: 2.127 ± 0.021	2.846IlePro: 2.846 ± 0.025	1.933IleGln: 1.933 ± 0.022	3.327IleArg: 3.327 ± 0.027	4.258IleSer: 4.258 ± 0.03	3.047IleThr: 3.047 ± 0.027	3.784IleVal: 3.784 ± 0.028	0.578IleTrp: 0.578 ± 0.01	1.632IleTyr: 1.632 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	3.749LysAla: 3.749 ± 0.032	1.169LysCys: 1.169 ± 0.018	2.996LysAsp: 2.996 ± 0.028	4.466LysGlu: 4.466 ± 0.045	2.085LysPhe: 2.085 ± 0.023	2.773LysGly: 2.773 ± 0.03	1.289LysHis: 1.289 ± 0.016	3.164LysIle: 3.164 ± 0.024	4.825LysLys: 4.825 ± 0.053	4.832LysLeu: 4.832 ± 0.037	1.676LysMet: 1.676 ± 0.022	2.564LysAsn: 2.564 ± 0.023	2.607LysPro: 2.607 ± 0.026	2.334LysGln: 2.334 ± 0.024	3.9LysArg: 3.9 ± 0.033	3.854LysSer: 3.854 ± 0.032	3.183LysThr: 3.183 ± 0.028	3.41LysVal: 3.41 ± 0.028	0.68LysTrp: 0.68 ± 0.013	1.827LysTyr: 1.827 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	6.398LeuAla: 6.398 ± 0.037	2.01LeuCys: 2.01 ± 0.019	4.604LeuAsp: 4.604 ± 0.032	5.59LeuGlu: 5.59 ± 0.046	3.937LeuPhe: 3.937 ± 0.034	4.431LeuGly: 4.431 ± 0.028	2.304LeuHis: 2.304 ± 0.022	4.666LeuIle: 4.666 ± 0.035	4.989LeuLys: 4.989 ± 0.039	8.951LeuLeu: 8.951 ± 0.059	2.256LeuMet: 2.256 ± 0.02	3.756LeuAsn: 3.756 ± 0.03	4.757LeuPro: 4.757 ± 0.039	3.633LeuGln: 3.633 ± 0.032	5.885LeuArg: 5.885 ± 0.036	7.107LeuSer: 7.107 ± 0.048	4.852LeuThr: 4.852 ± 0.035	5.518LeuVal: 5.518 ± 0.037	0.974LeuTrp: 0.974 ± 0.012	2.522LeuTyr: 2.522 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	1.863MetAla: 1.863 ± 0.02	0.52MetCys: 0.52 ± 0.011	1.534MetAsp: 1.534 ± 0.017	1.777MetGlu: 1.777 ± 0.021	1.081MetPhe: 1.081 ± 0.017	1.391MetGly: 1.391 ± 0.021	0.585MetHis: 0.585 ± 0.011	1.353MetIle: 1.353 ± 0.017	1.645MetLys: 1.645 ± 0.019	2.344MetLeu: 2.344 ± 0.021	0.849MetMet: 0.849 ± 0.015	1.18MetAsn: 1.18 ± 0.017	1.167MetPro: 1.167 ± 0.015	1.023MetGln: 1.023 ± 0.014	1.716MetArg: 1.716 ± 0.017	1.986MetSer: 1.986 ± 0.023	1.497MetThr: 1.497 ± 0.018	1.588MetVal: 1.588 ± 0.018	0.292MetTrp: 0.292 ± 0.007	0.73MetTyr: 0.73 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.94AsnAla: 2.94 ± 0.024	0.872AsnCys: 0.872 ± 0.015	2.298AsnAsp: 2.298 ± 0.024	2.863AsnGlu: 2.863 ± 0.027	1.729AsnPhe: 1.729 ± 0.019	2.925AsnGly: 2.925 ± 0.029	0.919AsnHis: 0.919 ± 0.012	2.443AsnIle: 2.443 ± 0.024	2.144AsnLys: 2.144 ± 0.021	3.454AsnLeu: 3.454 ± 0.025	1.101AsnMet: 1.101 ± 0.014	1.839AsnAsn: 1.839 ± 0.021	2.149AsnPro: 2.149 ± 0.022	1.465AsnGln: 1.465 ± 0.016	2.374AsnArg: 2.374 ± 0.02	3.114AsnSer: 3.114 ± 0.028	2.238AsnThr: 2.238 ± 0.024	2.998AsnVal: 2.998 ± 0.026	0.449AsnTrp: 0.449 ± 0.01	1.36AsnTyr: 1.36 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	3.508ProAla: 3.508 ± 0.038	0.869ProCys: 0.869 ± 0.017	2.467ProAsp: 2.467 ± 0.026	3.129ProGlu: 3.129 ± 0.027	1.989ProPhe: 1.989 ± 0.022	3.516ProGly: 3.516 ± 0.099	1.187ProHis: 1.187 ± 0.016	2.503ProIle: 2.503 ± 0.023	2.639ProLys: 2.639 ± 0.023	4.227ProLeu: 4.227 ± 0.033	1.141ProMet: 1.141 ± 0.017	1.977ProAsn: 1.977 ± 0.018	4.385ProPro: 4.385 ± 0.073	2.064ProGln: 2.064 ± 0.026	2.752ProArg: 2.752 ± 0.027	4.599ProSer: 4.599 ± 0.041	3.197ProThr: 3.197 ± 0.033	3.296ProVal: 3.296 ± 0.031	0.503ProTrp: 0.503 ± 0.01	1.451ProTyr: 1.451 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	2.478GlnAla: 2.478 ± 0.023	0.929GlnCys: 0.929 ± 0.02	1.497GlnAsp: 1.497 ± 0.018	2.298GlnGlu: 2.298 ± 0.026	1.591GlnPhe: 1.591 ± 0.02	1.804GlnGly: 1.804 ± 0.023	1.036GlnHis: 1.036 ± 0.016	2.15GlnIle: 2.15 ± 0.019	2.399GlnLys: 2.399 ± 0.025	3.97GlnLeu: 3.97 ± 0.034	1.248GlnMet: 1.248 ± 0.017	1.652GlnAsn: 1.652 ± 0.02	2.037GlnPro: 2.037 ± 0.03	2.368GlnGln: 2.368 ± 0.042	2.607GlnArg: 2.607 ± 0.027	2.649GlnSer: 2.649 ± 0.025	2.0GlnThr: 2.0 ± 0.025	2.186GlnVal: 2.186 ± 0.021	0.517GlnTrp: 0.517 ± 0.011	1.226GlnTyr: 1.226 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	3.926ArgAla: 3.926 ± 0.03	1.333ArgCys: 1.333 ± 0.019	3.137ArgAsp: 3.137 ± 0.03	4.08ArgGlu: 4.08 ± 0.034	2.501ArgPhe: 2.501 ± 0.022	3.214ArgGly: 3.214 ± 0.035	1.586ArgHis: 1.586 ± 0.016	3.455ArgIle: 3.455 ± 0.027	4.117ArgLys: 4.117 ± 0.036	5.676ArgLeu: 5.676 ± 0.037	1.695ArgMet: 1.695 ± 0.019	2.736ArgAsn: 2.736 ± 0.026	2.85ArgPro: 2.85 ± 0.026	2.47ArgGln: 2.47 ± 0.025	5.289ArgArg: 5.289 ± 0.048	4.709ArgSer: 4.709 ± 0.034	3.37ArgThr: 3.37 ± 0.027	3.613ArgVal: 3.613 ± 0.027	0.712ArgTrp: 0.712 ± 0.012	1.805ArgTyr: 1.805 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	5.434SerAla: 5.434 ± 0.039	1.597SerCys: 1.597 ± 0.022	4.139SerAsp: 4.139 ± 0.034	4.64SerGlu: 4.64 ± 0.035	3.245SerPhe: 3.245 ± 0.027	4.627SerGly: 4.627 ± 0.037	1.747SerHis: 1.747 ± 0.018	3.996SerIle: 3.996 ± 0.031	3.922SerLys: 3.922 ± 0.035	6.847SerLeu: 6.847 ± 0.047	1.908SerMet: 1.908 ± 0.019	3.083SerAsn: 3.083 ± 0.024	4.155SerPro: 4.155 ± 0.044	2.788SerGln: 2.788 ± 0.029	4.593SerArg: 4.593 ± 0.036	8.008SerSer: 8.008 ± 0.069	5.035SerThr: 5.035 ± 0.046	4.962SerVal: 4.962 ± 0.033	0.824SerTrp: 0.824 ± 0.013	2.16SerTyr: 2.16 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	4.179ThrAla: 4.179 ± 0.035	1.245ThrCys: 1.245 ± 0.02	2.708ThrAsp: 2.708 ± 0.023	3.291ThrGlu: 3.291 ± 0.031	2.305ThrPhe: 2.305 ± 0.022	3.212ThrGly: 3.212 ± 0.029	1.248ThrHis: 1.248 ± 0.016	3.245ThrIle: 3.245 ± 0.027	2.95ThrLys: 2.95 ± 0.027	4.874ThrLeu: 4.874 ± 0.035	1.404ThrMet: 1.404 ± 0.018	2.292ThrAsn: 2.292 ± 0.022	3.237ThrPro: 3.237 ± 0.036	1.919ThrGln: 1.919 ± 0.02	2.922ThrArg: 2.922 ± 0.022	4.866ThrSer: 4.866 ± 0.046	4.146ThrThr: 4.146 ± 0.063	4.174ThrVal: 4.174 ± 0.033	0.59ThrTrp: 0.59 ± 0.011	1.6ThrTyr: 1.6 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	4.886ValAla: 4.886 ± 0.031	1.486ValCys: 1.486 ± 0.022	3.944ValAsp: 3.944 ± 0.029	4.411ValGlu: 4.411 ± 0.031	2.856ValPhe: 2.856 ± 0.029	3.652ValGly: 3.652 ± 0.035	1.684ValHis: 1.684 ± 0.019	3.659ValIle: 3.659 ± 0.025	3.511ValLys: 3.511 ± 0.031	6.064ValLeu: 6.064 ± 0.04	1.622ValMet: 1.622 ± 0.019	2.773ValAsn: 2.773 ± 0.025	3.448ValPro: 3.448 ± 0.03	2.424ValGln: 2.424 ± 0.022	3.799ValArg: 3.799 ± 0.032	4.8ValSer: 4.8 ± 0.032	3.702ValThr: 3.702 ± 0.03	5.107ValVal: 5.107 ± 0.045	0.725ValTrp: 0.725 ± 0.013	1.967ValTyr: 1.967 ± 0.023	0.0ValXaa: 0.0 ± 0.0
Trp	0.669TrpAla: 0.669 ± 0.011	0.224TrpCys: 0.224 ± 0.007	0.583TrpAsp: 0.583 ± 0.011	0.655TrpGlu: 0.655 ± 0.012	0.481TrpPhe: 0.481 ± 0.009	0.529TrpGly: 0.529 ± 0.012	0.262TrpHis: 0.262 ± 0.007	0.715TrpIle: 0.715 ± 0.013	0.784TrpLys: 0.784 ± 0.012	1.051TrpLeu: 1.051 ± 0.015	0.394TrpMet: 0.394 ± 0.01	0.597TrpAsn: 0.597 ± 0.012	0.452TrpPro: 0.452 ± 0.01	0.441TrpGln: 0.441 ± 0.009	0.763TrpArg: 0.763 ± 0.012	0.828TrpSer: 0.828 ± 0.014	0.711TrpThr: 0.711 ± 0.011	0.585TrpVal: 0.585 ± 0.011	0.19TrpTrp: 0.19 ± 0.007	0.351TrpTyr: 0.351 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.011TyrAla: 2.011 ± 0.021	0.76TyrCys: 0.76 ± 0.015	1.824TyrAsp: 1.824 ± 0.021	1.886TyrGlu: 1.886 ± 0.018	1.376TyrPhe: 1.376 ± 0.019	2.098TyrGly: 2.098 ± 0.026	0.778TyrHis: 0.778 ± 0.012	1.501TyrIle: 1.501 ± 0.019	1.558TyrLys: 1.558 ± 0.019	2.629TyrLeu: 2.629 ± 0.024	0.812TyrMet: 0.812 ± 0.013	1.301TyrAsn: 1.301 ± 0.017	1.393TyrPro: 1.393 ± 0.019	1.099TyrGln: 1.099 ± 0.015	1.929TyrArg: 1.929 ± 0.023	2.214TyrSer: 2.214 ± 0.024	1.546TyrThr: 1.546 ± 0.019	1.974TyrVal: 1.974 ± 0.021	0.385TyrTrp: 0.385 ± 0.011	1.136TyrTyr: 1.136 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 19125 proteins (5133344 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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