Pepper virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; unclassified Carlavirus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5KTS4|A0A1W5KTS4_9VIRU TGB1 OS=Pepper virus A OX=1803898 GN=gp2 PE=4 SV=1
MM1 pKa = 7.4LCVINKK7 pKa = 9.52LVDD10 pKa = 3.59CGFSRR15 pKa = 11.84VGPSLNKK22 pKa = 9.77PLIINCVPGGGKK34 pKa = 6.45TTIIRR39 pKa = 11.84QLLEE43 pKa = 4.19EE44 pKa = 4.45FDD46 pKa = 4.26HH47 pKa = 6.72LVAYY51 pKa = 6.8TTASPDD57 pKa = 4.09PINLKK62 pKa = 10.48GNQIKK67 pKa = 10.42ALPATPEE74 pKa = 3.84EE75 pKa = 4.36GKK77 pKa = 10.77LVILDD82 pKa = 4.3EE83 pKa = 4.31YY84 pKa = 11.45QNLAKK89 pKa = 10.74LPDD92 pKa = 3.43WVDD95 pKa = 2.89IAFGDD100 pKa = 4.2PLQSCNPNLLSADD113 pKa = 3.5FLSYY117 pKa = 9.0RR118 pKa = 11.84THH120 pKa = 6.56RR121 pKa = 11.84FGRR124 pKa = 11.84NTCGLLGRR132 pKa = 11.84LGFRR136 pKa = 11.84VEE138 pKa = 4.51SDD140 pKa = 3.8LEE142 pKa = 4.23DD143 pKa = 3.47SVVFEE148 pKa = 4.55GLFEE152 pKa = 4.78GEE154 pKa = 4.54LEE156 pKa = 4.22GQILCCEE163 pKa = 4.18QEE165 pKa = 4.21VEE167 pKa = 4.32EE168 pKa = 5.0LLTSHH173 pKa = 6.31SVEE176 pKa = 3.96FLTPRR181 pKa = 11.84TAQGKK186 pKa = 5.81TFEE189 pKa = 4.69VVTFCCSEE197 pKa = 3.99RR198 pKa = 11.84PLANNLHH205 pKa = 6.79LYY207 pKa = 8.88LICLTRR213 pKa = 11.84HH214 pKa = 4.49TSKK217 pKa = 10.82LRR219 pKa = 11.84ILNPEE224 pKa = 3.76GAAGFFF230 pKa = 3.95

Molecular weight: 25.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5KTS4|A0A1W5KTS4_9VIRU TGB1 OS=Pepper virus A OX=1803898 GN=gp2 PE=4 SV=1
MM1 pKa = 8.07IPMKK5 pKa = 10.28EE6 pKa = 3.8RR7 pKa = 11.84AVALAIAASRR17 pKa = 11.84LGRR20 pKa = 11.84HH21 pKa = 5.87LPFDD25 pKa = 3.63VCLYY29 pKa = 10.15ICSFNYY35 pKa = 9.55KK36 pKa = 10.05DD37 pKa = 3.84CVGSGRR43 pKa = 11.84SSYY46 pKa = 10.48ARR48 pKa = 11.84KK49 pKa = 9.6RR50 pKa = 11.84RR51 pKa = 11.84AKK53 pKa = 10.22SIRR56 pKa = 11.84RR57 pKa = 11.84CHH59 pKa = 5.5RR60 pKa = 11.84CFRR63 pKa = 11.84VRR65 pKa = 11.84PGFYY69 pKa = 9.3FTTRR73 pKa = 11.84CDD75 pKa = 3.77GVSCKK80 pKa = 10.24PGISYY85 pKa = 7.76PIWVEE90 pKa = 3.15LFIRR94 pKa = 11.84FGIPRR99 pKa = 11.84SMSTSGTEE107 pKa = 3.76EE108 pKa = 3.9RR109 pKa = 11.84GG110 pKa = 3.36

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2767

107

2037

553.4

62.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.228 ± 1.051

2.891 ± 0.616

5.132 ± 0.481

6.397 ± 0.889

4.59 ± 0.567

6.397 ± 0.525

2.458 ± 0.264

5.168 ± 0.301

5.71 ± 0.769

10.264 ± 1.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.988 ± 0.363

4.192 ± 0.528

4.301 ± 0.851

3.433 ± 0.453

5.746 ± 1.122

7.409 ± 0.582

5.24 ± 0.535

6.903 ± 0.332

0.976 ± 0.199

3.578 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski