Apis mellifera associated microvirus 36

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3S8UUC2|A0A3S8UUC2_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 36 OX=2494765 PE=4 SV=1
MM1 pKa = 7.38NLFNQVQIRR10 pKa = 11.84RR11 pKa = 11.84PKK13 pKa = 9.89RR14 pKa = 11.84NKK16 pKa = 9.61FDD18 pKa = 3.91LSHH21 pKa = 6.29EE22 pKa = 4.44KK23 pKa = 10.49KK24 pKa = 10.77LSFNMGDD31 pKa = 5.32LIPIMCQEE39 pKa = 5.36IIPGDD44 pKa = 3.59NFRR47 pKa = 11.84VNTEE51 pKa = 3.59TFMRR55 pKa = 11.84LAPMIAPVMHH65 pKa = 6.62RR66 pKa = 11.84VNVYY70 pKa = 5.97THH72 pKa = 6.06YY73 pKa = 10.7FFVPTRR79 pKa = 11.84IIWDD83 pKa = 3.62EE84 pKa = 3.92FQDD87 pKa = 4.81FITGGRR93 pKa = 11.84EE94 pKa = 4.23GTSMPISPFFGQSTVEE110 pKa = 3.98NARR113 pKa = 11.84GSLLDD118 pKa = 4.0YY119 pKa = 10.19MGLPLWPSGSSNDD132 pKa = 3.68TQISVLPLRR141 pKa = 11.84AYY143 pKa = 7.91QTVWDD148 pKa = 4.8EE149 pKa = 4.1YY150 pKa = 11.32FRR152 pKa = 11.84DD153 pKa = 3.81QNLTPSLDD161 pKa = 3.13ISKK164 pKa = 10.86ASGVMPADD172 pKa = 3.6AEE174 pKa = 4.3LFKK177 pKa = 11.18LLEE180 pKa = 3.95LRR182 pKa = 11.84KK183 pKa = 9.69RR184 pKa = 11.84AWEE187 pKa = 3.7KK188 pKa = 11.28DD189 pKa = 3.61YY190 pKa = 9.27FTSALPWSQRR200 pKa = 11.84GNEE203 pKa = 4.17ILLPMTPEE211 pKa = 3.55VNYY214 pKa = 10.57RR215 pKa = 11.84PTSDD219 pKa = 3.46VYY221 pKa = 11.68YY222 pKa = 10.74EE223 pKa = 5.75DD224 pKa = 3.57GTQANNSSWGIGEE237 pKa = 4.36GPAGEE242 pKa = 4.74DD243 pKa = 3.49GKK245 pKa = 10.79IVPTPGANILQMRR258 pKa = 11.84IEE260 pKa = 4.9NIDD263 pKa = 3.83SIDD266 pKa = 3.92NVTTTINDD274 pKa = 2.95LRR276 pKa = 11.84RR277 pKa = 11.84AVRR280 pKa = 11.84LQEE283 pKa = 3.92WLEE286 pKa = 3.87KK287 pKa = 9.97NARR290 pKa = 11.84GGARR294 pKa = 11.84YY295 pKa = 9.32IEE297 pKa = 4.53QILHH301 pKa = 6.04HH302 pKa = 6.67FGVTSSDD309 pKa = 3.08ARR311 pKa = 11.84LQRR314 pKa = 11.84PEE316 pKa = 3.75YY317 pKa = 10.49LGGGKK322 pKa = 9.57QPVVISEE329 pKa = 4.08VLQTSEE335 pKa = 4.55TLSTPQGTMAGHH347 pKa = 6.45GVSAGTSNGFNRR359 pKa = 11.84SFEE362 pKa = 3.89EE363 pKa = 3.49HH364 pKa = 6.83GYY366 pKa = 10.35VIGVMSVLPRR376 pKa = 11.84TAYY379 pKa = 9.97QDD381 pKa = 3.59GVNKK385 pKa = 9.2MWSRR389 pKa = 11.84TDD391 pKa = 2.89KK392 pKa = 10.86FDD394 pKa = 4.08YY395 pKa = 10.05FWPEE399 pKa = 3.44LANIGEE405 pKa = 4.22QEE407 pKa = 4.22VKK409 pKa = 10.43IKK411 pKa = 10.35EE412 pKa = 4.33LYY414 pKa = 10.67LNPTGAQTNDD424 pKa = 2.76TFGYY428 pKa = 7.52QSRR431 pKa = 11.84YY432 pKa = 10.33AEE434 pKa = 4.25YY435 pKa = 10.17KK436 pKa = 9.33YY437 pKa = 10.4MPSTVHH443 pKa = 6.88GDD445 pKa = 3.03FRR447 pKa = 11.84TNLDD451 pKa = 3.39YY452 pKa = 11.19WHH454 pKa = 7.1MGRR457 pKa = 11.84KK458 pKa = 9.43FSTEE462 pKa = 3.42PVLNEE467 pKa = 4.43DD468 pKa = 5.84FITADD473 pKa = 3.14PTHH476 pKa = 7.26RR477 pKa = 11.84IFAVTDD483 pKa = 3.8PDD485 pKa = 3.47EE486 pKa = 4.78HH487 pKa = 8.32KK488 pKa = 10.75LYY490 pKa = 10.33CQLYY494 pKa = 9.09HH495 pKa = 7.31RR496 pKa = 11.84IDD498 pKa = 3.64ALRR501 pKa = 11.84PMPYY505 pKa = 10.05FGTPTLL511 pKa = 3.89

Molecular weight:
58.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3Q8U647|A0A3Q8U647_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 36 OX=2494765 PE=4 SV=1
MM1 pKa = 6.59YY2 pKa = 10.35QVGVVLKK9 pKa = 7.92FAPYY13 pKa = 10.67SPFNPTSCRR22 pKa = 11.84PTSQRR27 pKa = 11.84HH28 pKa = 4.75AATLIPRR35 pKa = 11.84GVGATIGRR43 pKa = 11.84RR44 pKa = 11.84WAVDD48 pKa = 3.27RR49 pKa = 11.84GRR51 pKa = 11.84KK52 pKa = 8.8IKK54 pKa = 9.62PCKK57 pKa = 9.65GFKK60 pKa = 9.85IGLKK64 pKa = 9.9TNTLPLGAPGRR75 pKa = 11.84TNATCHH81 pKa = 6.68DD82 pKa = 4.13GYY84 pKa = 11.5GRR86 pKa = 11.84DD87 pKa = 4.25GLNPWALEE95 pKa = 3.94APLPLNGII103 pKa = 4.31

Molecular weight:
11.11 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1270

103

511

254.0

28.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.402 ± 1.387

0.551 ± 0.261

4.803 ± 0.655

6.929 ± 1.267

3.78 ± 0.708

7.087 ± 0.836

2.835 ± 0.909

5.433 ± 0.534

6.772 ± 2.171

7.638 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.913 ± 0.424

6.063 ± 1.023

5.591 ± 0.939

4.252 ± 0.714

6.378 ± 0.638

5.118 ± 0.497

6.457 ± 0.502

4.016 ± 0.681

1.732 ± 0.341

4.252 ± 0.388

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski