Buchnera aphidicola (Anoecia oenotherae)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6XVL6|A0A4D6XVL6_9GAMM Histidinol-phosphate aminotransferase OS=Buchnera aphidicola (Anoecia oenotherae) OX=1241833 GN=hisC PE=3 SV=1
MM1 pKa = 7.53LHH3 pKa = 6.38TLMKK7 pKa = 10.14SPYY10 pKa = 10.0FIDD13 pKa = 4.83LDD15 pKa = 4.08TMFLMVDD22 pKa = 3.99LNDD25 pKa = 5.57DD26 pKa = 4.2IIMLQDD32 pKa = 3.34GVIFAVDD39 pKa = 3.42EE40 pKa = 4.47NFVLTYY46 pKa = 10.21KK47 pKa = 10.63SYY49 pKa = 11.49INIIYY54 pKa = 9.78ILKK57 pKa = 9.98EE58 pKa = 3.83DD59 pKa = 3.85LEE61 pKa = 4.6ARR63 pKa = 11.84GLKK66 pKa = 10.14NINSSFKK73 pKa = 10.47VINYY77 pKa = 8.73IQFVQLTFKK86 pKa = 9.94HH87 pKa = 4.62TTQMNWW93 pKa = 2.48

Molecular weight: 11.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6XR12|A0A4D6XR12_9GAMM Signal recognition particle receptor FtsY OS=Buchnera aphidicola (Anoecia oenotherae) OX=1241833 GN=ftsY PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.28RR12 pKa = 11.84NKK14 pKa = 8.29THH16 pKa = 6.59GFRR19 pKa = 11.84NRR21 pKa = 11.84MSTKK25 pKa = 9.45NGRR28 pKa = 11.84AILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 11.05SRR39 pKa = 11.84IQLTVSSKK47 pKa = 10.98

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

444

0

444

142166

38

1407

320.2

36.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.763 ± 0.095

1.298 ± 0.035

3.833 ± 0.074

4.884 ± 0.095

5.295 ± 0.121

4.965 ± 0.116

1.943 ± 0.042

12.018 ± 0.121

11.402 ± 0.148

9.74 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.042

7.962 ± 0.108

2.811 ± 0.05

2.773 ± 0.057

3.38 ± 0.075

7.405 ± 0.082

4.732 ± 0.064

4.948 ± 0.091

0.829 ± 0.034

3.947 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski