Mycobacterium phage Pepe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0LEA1|A0A0P0LEA1_9CAUD Tail protein OS=Mycobacterium phage Pepe OX=1735466 GN=SEA_PEPE_6 PE=4 SV=1
MM1 pKa = 7.06YY2 pKa = 10.59VVTDD6 pKa = 4.24PDD8 pKa = 3.65GDD10 pKa = 4.04EE11 pKa = 4.22YY12 pKa = 11.25TVTDD16 pKa = 3.97TEE18 pKa = 5.83FDD20 pKa = 4.08DD21 pKa = 3.47GWYY24 pKa = 9.36WVVSPLSEE32 pKa = 4.67FAPCHH37 pKa = 6.69LDD39 pKa = 3.74LGLHH43 pKa = 4.69RR44 pKa = 11.84TKK46 pKa = 10.64RR47 pKa = 11.84DD48 pKa = 3.07AVAALAEE55 pKa = 4.56FVSS58 pKa = 4.03

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0M692|A0A0P0M692_9CAUD Uncharacterized protein OS=Mycobacterium phage Pepe OX=1735466 GN=SEA_PEPE_31 PE=4 SV=1
MM1 pKa = 7.35SWDD4 pKa = 3.28SSDD7 pKa = 3.35RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 4.4RR11 pKa = 11.84LPADD15 pKa = 3.28WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.46RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.29PDD37 pKa = 3.55ICTGMAAEE45 pKa = 4.27VDD47 pKa = 3.54HH48 pKa = 6.7VRR50 pKa = 11.84YY51 pKa = 9.6RR52 pKa = 11.84DD53 pKa = 3.64EE54 pKa = 4.46EE55 pKa = 4.54SPLQASCRR63 pKa = 11.84PCHH66 pKa = 5.76ARR68 pKa = 11.84KK69 pKa = 9.57SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.63PGRR95 pKa = 11.84RR96 pKa = 11.84NKK98 pKa = 10.77EE99 pKa = 3.81EE100 pKa = 3.84TT101 pKa = 3.39

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

15119

27

943

175.8

19.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.087 ± 0.475

0.602 ± 0.101

6.416 ± 0.259

6.813 ± 0.297

3.254 ± 0.169

8.433 ± 0.39

1.964 ± 0.162

4.881 ± 0.221

4.14 ± 0.209

8.744 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.077 ± 0.123

3.122 ± 0.168

5.305 ± 0.27

3.307 ± 0.26

6.323 ± 0.416

5.807 ± 0.23

6.356 ± 0.279

7.467 ± 0.209

2.09 ± 0.135

2.811 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski