Tepidamorphus gemmatus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Tepidamorphaceae; Tepidamorphus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2UXZ6|A0A4V2UXZ6_9RHIZ CHAD domain-containing protein OS=Tepidamorphus gemmatus OX=747076 GN=EDC22_11320 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.32EE4 pKa = 4.48CCEE7 pKa = 4.08DD8 pKa = 3.11VHH10 pKa = 8.8VYY12 pKa = 10.66VLVGRR17 pKa = 11.84ASRR20 pKa = 11.84AGPLADD26 pKa = 5.17GYY28 pKa = 11.4DD29 pKa = 3.56PSEE32 pKa = 4.86DD33 pKa = 3.62EE34 pKa = 4.17NDD36 pKa = 3.56AVPVNVLLTATDD48 pKa = 3.21EE49 pKa = 4.36GAAISRR55 pKa = 11.84ALAALANEE63 pKa = 5.14GYY65 pKa = 10.54GRR67 pKa = 11.84AEE69 pKa = 4.46LDD71 pKa = 3.45RR72 pKa = 11.84IGIVDD77 pKa = 4.86GEE79 pKa = 4.27PDD81 pKa = 4.2DD82 pKa = 4.0PTFAAAYY89 pKa = 7.91WDD91 pKa = 4.85AIDD94 pKa = 5.36GEE96 pKa = 4.64VAVIAFHH103 pKa = 6.84GG104 pKa = 3.8

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3MHJ1|A0A4R3MHJ1_9RHIZ Head-tail adaptor OS=Tepidamorphus gemmatus OX=747076 GN=EDC22_102353 PE=4 SV=1
MM1 pKa = 7.66AVRR4 pKa = 11.84SILFNIAFYY13 pKa = 11.31LNIIVWMIGCTPMLLLPRR31 pKa = 11.84RR32 pKa = 11.84WTIGCVHH39 pKa = 6.16GWARR43 pKa = 11.84CSTWLLEE50 pKa = 4.45VIGGVRR56 pKa = 11.84VEE58 pKa = 4.2VRR60 pKa = 11.84GGLPRR65 pKa = 11.84RR66 pKa = 11.84GMPLLVAAKK75 pKa = 8.63HH76 pKa = 4.81QSVFEE81 pKa = 4.18TFALLPYY88 pKa = 10.33FSDD91 pKa = 3.36PAFILKK97 pKa = 10.21RR98 pKa = 11.84EE99 pKa = 4.3LTWIPLFGWWCRR111 pKa = 11.84KK112 pKa = 7.44MRR114 pKa = 11.84MIPVDD119 pKa = 3.52RR120 pKa = 11.84GRR122 pKa = 11.84GATALKK128 pKa = 10.81DD129 pKa = 3.48MARR132 pKa = 11.84RR133 pKa = 11.84AHH135 pKa = 6.74AEE137 pKa = 3.76AARR140 pKa = 11.84GRR142 pKa = 11.84QIVIFPEE149 pKa = 4.5GTRR152 pKa = 11.84RR153 pKa = 11.84PPGAPPQYY161 pKa = 9.79KK162 pKa = 9.9QGVSLLYY169 pKa = 9.89ATMQVPCLPVALNSGLFWPRR189 pKa = 11.84RR190 pKa = 11.84KK191 pKa = 7.93WQRR194 pKa = 11.84YY195 pKa = 6.61PGTILVEE202 pKa = 4.36FLEE205 pKa = 5.32PIPPGLDD212 pKa = 2.39RR213 pKa = 11.84EE214 pKa = 4.56AFRR217 pKa = 11.84EE218 pKa = 3.89RR219 pKa = 11.84LEE221 pKa = 4.29TAIEE225 pKa = 4.08TASDD229 pKa = 3.64RR230 pKa = 11.84LLGEE234 pKa = 4.22AAAAPNPPPIPDD246 pKa = 3.31EE247 pKa = 3.93ARR249 pKa = 11.84ARR251 pKa = 11.84LEE253 pKa = 3.99GMVAAATPTTPPPRR267 pKa = 11.84RR268 pKa = 11.84QAGG271 pKa = 3.25

Molecular weight: 30.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3554

0

3554

1129158

28

1830

317.7

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.506 ± 0.063

0.86 ± 0.014

5.681 ± 0.032

5.79 ± 0.034

3.545 ± 0.022

8.834 ± 0.034

1.966 ± 0.023

5.27 ± 0.03

2.307 ± 0.03

10.075 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.019

2.124 ± 0.022

5.476 ± 0.033

2.628 ± 0.021

8.317 ± 0.043

4.739 ± 0.023

5.109 ± 0.026

7.87 ± 0.036

1.341 ± 0.018

2.132 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski