Agromyces sp. KACC 19306

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1YC09|A0A5C1YC09_9MICO 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Agromyces sp. KACC 19306 OX=2592652 GN=ispD PE=3 SV=1
MM1 pKa = 7.78ANRR4 pKa = 11.84SAEE7 pKa = 3.95ASIAPPQQPLSCEE20 pKa = 3.99SLKK23 pKa = 10.85PSSEE27 pKa = 4.21LEE29 pKa = 3.98DD30 pKa = 3.06RR31 pKa = 11.84TAYY34 pKa = 10.63LDD36 pKa = 4.0CLASQNAGLRR46 pKa = 11.84SFAAAAAVDD55 pKa = 4.19EE56 pKa = 4.96NGNPDD61 pKa = 4.18LPDD64 pKa = 3.43SCGIDD69 pKa = 2.84IALILDD75 pKa = 3.52KK76 pKa = 11.11SGSIGGAGIANLKK89 pKa = 9.57SAANAFTGALVDD101 pKa = 4.53LGSQVSVVSFDD112 pKa = 3.06QDD114 pKa = 3.21AYY116 pKa = 11.58LLLPATDD123 pKa = 3.79LTSGNLGTIQGSYY136 pKa = 10.59AGLVSDD142 pKa = 5.52GWTNWKK148 pKa = 10.23RR149 pKa = 11.84GLEE152 pKa = 4.1VGLGTFGGFDD162 pKa = 3.56DD163 pKa = 4.37TVEE166 pKa = 4.1LTVVITDD173 pKa = 3.97GNPNTVNPANGGQFPDD189 pKa = 4.56GSPGALDD196 pKa = 3.64PAVLEE201 pKa = 4.34ANAIKK206 pKa = 9.11GTPSHH211 pKa = 6.03VFVVGVGPSVSTGPIVAISGAEE233 pKa = 3.82VLAADD238 pKa = 4.81GSNINTADD246 pKa = 3.48YY247 pKa = 7.46MTTTSYY253 pKa = 10.17ATLAEE258 pKa = 4.41DD259 pKa = 3.79LRR261 pKa = 11.84AVATALCGGTVVVHH275 pKa = 6.35KK276 pKa = 10.81SVDD279 pKa = 3.57GVATGGWGFEE289 pKa = 4.35TPTTDD294 pKa = 3.09VTPAALTTLADD305 pKa = 3.4GTGIFEE311 pKa = 4.36VEE313 pKa = 4.62GYY315 pKa = 9.88DD316 pKa = 3.25AQTDD320 pKa = 3.7VRR322 pKa = 11.84TVTITEE328 pKa = 4.03EE329 pKa = 3.83TRR331 pKa = 11.84PNHH334 pKa = 5.61GLEE337 pKa = 4.06NLYY340 pKa = 10.96CEE342 pKa = 4.97LNGEE346 pKa = 4.35QVPITHH352 pKa = 7.64DD353 pKa = 3.45EE354 pKa = 4.42GSPTWSVDD362 pKa = 3.14VGKK365 pKa = 10.76LDD367 pKa = 4.9IVHH370 pKa = 6.47CWVEE374 pKa = 3.9NEE376 pKa = 4.01TAKK379 pKa = 10.63WEE381 pKa = 4.22VTKK384 pKa = 10.73TSDD387 pKa = 3.86PPSGTAVEE395 pKa = 4.51AGDD398 pKa = 4.2FIDD401 pKa = 3.77YY402 pKa = 8.59TLHH405 pKa = 6.09IRR407 pKa = 11.84HH408 pKa = 6.4LSGPNVTDD416 pKa = 4.33FDD418 pKa = 4.37IDD420 pKa = 4.29DD421 pKa = 6.01DD422 pKa = 4.23ISQLAPYY429 pKa = 10.17VSFVGLLTPAPVVSSDD445 pKa = 2.76WDD447 pKa = 3.76GTPGKK452 pKa = 10.3YY453 pKa = 9.94LLKK456 pKa = 10.48LAQLTKK462 pKa = 10.91AEE464 pKa = 4.32TLNIRR469 pKa = 11.84YY470 pKa = 8.81RR471 pKa = 11.84VQVDD475 pKa = 3.02ADD477 pKa = 3.91VPPGSVLRR485 pKa = 11.84NVVLTNCPAPAEE497 pKa = 4.46GEE499 pKa = 3.83PDD501 pKa = 3.3LCVTEE506 pKa = 4.62NPTPGWEE513 pKa = 3.83LRR515 pKa = 11.84KK516 pKa = 10.17YY517 pKa = 10.52SNPPSGSVVYY527 pKa = 9.78PGYY530 pKa = 10.59EE531 pKa = 3.67VTYY534 pKa = 10.51EE535 pKa = 3.58LDD537 pKa = 3.31AYY539 pKa = 10.3NFSEE543 pKa = 4.48APVIGGIATDD553 pKa = 4.37DD554 pKa = 4.11LSDD557 pKa = 3.7VLDD560 pKa = 3.88NATIDD565 pKa = 3.63EE566 pKa = 5.07LGPGLTLEE574 pKa = 4.54GTTLTWAVPDD584 pKa = 4.67LEE586 pKa = 6.13PGDD589 pKa = 4.43SVSTSYY595 pKa = 10.46TVIVNDD601 pKa = 4.49DD602 pKa = 2.94AWGQTLTNVVTPEE615 pKa = 4.19YY616 pKa = 9.83PGSCPPPPQDD626 pKa = 3.34GEE628 pKa = 4.7VVVLAEE634 pKa = 4.59GEE636 pKa = 4.48CTTDD640 pKa = 5.03HH641 pKa = 6.61EE642 pKa = 4.81VADD645 pKa = 4.27PNLRR649 pKa = 11.84IQKK652 pKa = 9.61LWEE655 pKa = 4.38HH656 pKa = 7.23DD657 pKa = 3.76GGDD660 pKa = 3.73NPVDD664 pKa = 4.18SGDD667 pKa = 4.53DD668 pKa = 3.62PPDD671 pKa = 3.86VISYY675 pKa = 10.72AIGVWNSGNAPVEE688 pKa = 4.31NPVVTDD694 pKa = 3.65TLPEE698 pKa = 4.2GLTFVPGSEE707 pKa = 4.19VVPAGWVLDD716 pKa = 3.8ASVPGQVTFSQEE728 pKa = 3.85TPGPFDD734 pKa = 5.11PIAYY738 pKa = 8.49PGVIFEE744 pKa = 4.72FDD746 pKa = 3.73VEE748 pKa = 4.45VGEE751 pKa = 5.0LAQPDD756 pKa = 4.24PTVPIPDD763 pKa = 4.38LVNTACVAGEE773 pKa = 4.31IPPQEE778 pKa = 4.43PLDD781 pKa = 3.81VPLRR785 pKa = 11.84ALAIAFDD792 pKa = 4.39EE793 pKa = 4.94EE794 pKa = 4.7PPADD798 pKa = 4.96DD799 pKa = 4.98PDD801 pKa = 4.22IDD803 pKa = 5.58GPLADD808 pKa = 5.21CDD810 pKa = 3.83EE811 pKa = 4.85ASTPVKK817 pKa = 10.52SVALDD822 pKa = 3.8GAAQCVNDD830 pKa = 4.64TPWFTYY836 pKa = 10.49AVTPYY841 pKa = 11.08NMEE844 pKa = 4.23APLPAVALIWWTLDD858 pKa = 2.86AYY860 pKa = 9.8EE861 pKa = 4.8NRR863 pKa = 11.84DD864 pKa = 3.6PSIDD868 pKa = 3.75AADD871 pKa = 3.91EE872 pKa = 4.2AALLADD878 pKa = 4.46GASQVDD884 pKa = 4.3YY885 pKa = 11.47VVIPPDD891 pKa = 3.53WAPGDD896 pKa = 3.85TLAGQQLWPGAEE908 pKa = 3.78VDD910 pKa = 4.63AEE912 pKa = 4.42GNPIDD917 pKa = 3.81WPGWTLLPDD926 pKa = 4.12GTWVLDD932 pKa = 3.74PDD934 pKa = 3.79APFYY938 pKa = 10.77DD939 pKa = 3.87LRR941 pKa = 11.84DD942 pKa = 3.54EE943 pKa = 4.35AVVEE947 pKa = 4.08IRR949 pKa = 11.84VNPSTDD955 pKa = 3.02VITAYY960 pKa = 9.85PPPTPNCNAAPPEE973 pKa = 4.22NPGPGPGPNPAGGSGSRR990 pKa = 11.84IAGTGFAGGWLVPFAVILLMGGALVAGRR1018 pKa = 11.84SWVRR1022 pKa = 11.84RR1023 pKa = 11.84RR1024 pKa = 11.84GLEE1027 pKa = 3.58QDD1029 pKa = 3.07

Molecular weight: 107.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1YDK2|A0A5C1YDK2_9MICO Protein translocase subunit SecA OS=Agromyces sp. KACC 19306 OX=2592652 GN=secA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3349

0

3349

1141035

32

3214

340.7

36.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.274 ± 0.068

0.466 ± 0.01

6.422 ± 0.035

5.719 ± 0.037

3.125 ± 0.024

9.182 ± 0.036

1.94 ± 0.017

4.295 ± 0.03

1.628 ± 0.029

10.006 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.566 ± 0.015

1.785 ± 0.024

5.635 ± 0.031

2.57 ± 0.022

7.654 ± 0.051

5.276 ± 0.025

5.774 ± 0.038

9.223 ± 0.046

1.556 ± 0.02

1.903 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski