Lactococcus phage 1706

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fremauxvirus; Lactococcus virus 1706

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2BTP0|B2BTP0_9CAUD Uncharacterized protein OS=Lactococcus phage 1706 OX=475178 PE=4 SV=1
MM1 pKa = 7.77LSTSDD6 pKa = 3.59NPYY9 pKa = 10.84NPWTQYY15 pKa = 11.36DD16 pKa = 3.33KK17 pKa = 10.82WYY19 pKa = 10.3DD20 pKa = 2.81WDD22 pKa = 3.89TTHH25 pKa = 7.47GYY27 pKa = 7.68NTSQYY32 pKa = 10.19IARR35 pKa = 11.84LANTLYY41 pKa = 11.49SMDD44 pKa = 3.87EE45 pKa = 3.94DD46 pKa = 4.89AEE48 pKa = 4.53LEE50 pKa = 4.53VYY52 pKa = 9.64QAAVDD57 pKa = 5.21AIMEE61 pKa = 4.18QNLTGNYY68 pKa = 9.8VIVPKK73 pKa = 9.38PTDD76 pKa = 3.63YY77 pKa = 11.65VEE79 pKa = 5.48ADD81 pKa = 3.09ASLYY85 pKa = 9.71EE86 pKa = 4.09

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2BTN9|B2BTN9_9CAUD Structural protein OS=Lactococcus phage 1706 OX=475178 PE=4 SV=1
MM1 pKa = 7.42INDD4 pKa = 3.49KK5 pKa = 10.29RR6 pKa = 11.84KK7 pKa = 10.17HH8 pKa = 4.5NARR11 pKa = 11.84PKK13 pKa = 10.16KK14 pKa = 10.15QLLIDD19 pKa = 3.73NQTILDD25 pKa = 3.81LHH27 pKa = 6.27YY28 pKa = 10.82LDD30 pKa = 4.5RR31 pKa = 11.84LNYY34 pKa = 9.55RR35 pKa = 11.84AIAEE39 pKa = 4.22KK40 pKa = 10.58AGCSKK45 pKa = 9.41NTIMRR50 pKa = 11.84IIKK53 pKa = 9.98NAGEE57 pKa = 4.13FPQEE61 pKa = 3.64YY62 pKa = 8.53WDD64 pKa = 4.11FRR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 9.5ILL70 pKa = 4.33

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

17201

61

1995

226.3

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.122 ± 0.709

0.39 ± 0.068

6.662 ± 0.349

6.192 ± 0.532

3.645 ± 0.194

6.744 ± 0.527

1.622 ± 0.151

7.575 ± 0.244

8.023 ± 0.408

7.936 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.14

6.43 ± 0.257

3.296 ± 0.197

3.744 ± 0.226

3.401 ± 0.29

6.151 ± 0.379

6.604 ± 0.327

6.267 ± 0.208

1.308 ± 0.129

4.163 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski