Methylophaga thiooxydans DMS010

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Methylophaga; Methylophaga thiooxydans

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2492 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0N7W6|C0N7W6_9GAMM Chaperone SurA OS=Methylophaga thiooxydans DMS010 OX=637616 GN=surA PE=3 SV=1
MM1 pKa = 7.65WFLLFDD7 pKa = 4.79RR8 pKa = 11.84PNKK11 pKa = 10.55AYY13 pKa = 10.55FSNNRR18 pKa = 11.84QAGFTLVEE26 pKa = 3.88ILLVIVLLGIVSIAAINAFSGNEE49 pKa = 3.65DD50 pKa = 3.09QARR53 pKa = 11.84QNVTRR58 pKa = 11.84LEE60 pKa = 3.96MAEE63 pKa = 4.04LQKK66 pKa = 11.42ALLQFRR72 pKa = 11.84RR73 pKa = 11.84DD74 pKa = 3.66NRR76 pKa = 11.84EE77 pKa = 3.94LPCMVYY83 pKa = 10.23RR84 pKa = 11.84QGDD87 pKa = 4.03FSPNTVNDD95 pKa = 3.52VNAHH99 pKa = 5.1EE100 pKa = 4.86NYY102 pKa = 10.72NDD104 pKa = 3.4FTVGFDD110 pKa = 3.84LTPLPADD117 pKa = 4.8AIAWQDD123 pKa = 3.11WCQEE127 pKa = 3.94NYY129 pKa = 10.6NNSAGEE135 pKa = 4.3TIASNALVMLNQFPYY150 pKa = 10.6DD151 pKa = 3.15IAEE154 pKa = 4.06FDD156 pKa = 3.7FLLWNGSTQQGWNGAYY172 pKa = 9.5ISQEE176 pKa = 3.79GLTDD180 pKa = 3.01SWGNAYY186 pKa = 9.57ILLDD190 pKa = 3.57PEE192 pKa = 5.13LIYY195 pKa = 10.4GARR198 pKa = 11.84YY199 pKa = 9.15RR200 pKa = 11.84CLINSGGDD208 pKa = 3.46DD209 pKa = 3.75YY210 pKa = 11.95DD211 pKa = 3.68ATGDD215 pKa = 4.0LYY217 pKa = 11.41EE218 pKa = 5.19CLTPSDD224 pKa = 3.97VGFVAADD231 pKa = 4.03HH232 pKa = 6.64ILPADD237 pKa = 3.68TARR240 pKa = 11.84IISPGPDD247 pKa = 3.14GEE249 pKa = 4.57IDD251 pKa = 3.48PDD253 pKa = 3.62YY254 pKa = 11.15DD255 pKa = 4.14ANPVSGNDD263 pKa = 3.33PCIAEE268 pKa = 4.34GDD270 pKa = 4.03DD271 pKa = 6.29LILCLMRR278 pKa = 6.29

Molecular weight: 30.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0N8V6|C0N8V6_9GAMM zf-CHCC domain-containing protein OS=Methylophaga thiooxydans DMS010 OX=637616 GN=MDMS009_2558 PE=4 SV=1
MM1 pKa = 7.32NEE3 pKa = 3.84STIVLLVLSLLGWLWWDD20 pKa = 3.17SRR22 pKa = 11.84GVAEE26 pKa = 4.77RR27 pKa = 11.84ATIAARR33 pKa = 11.84AYY35 pKa = 9.8CGNAGVSFLNDD46 pKa = 3.06TVAWQKK52 pKa = 9.83MRR54 pKa = 11.84LKK56 pKa = 10.59RR57 pKa = 11.84NRR59 pKa = 11.84QGRR62 pKa = 11.84MQLHH66 pKa = 5.1RR67 pKa = 11.84TYY69 pKa = 10.61FFEE72 pKa = 4.83FASDD76 pKa = 3.18MQQRR80 pKa = 11.84YY81 pKa = 9.01RR82 pKa = 11.84GQIIMLGKK90 pKa = 10.03KK91 pKa = 9.69VEE93 pKa = 4.36SVSLDD98 pKa = 3.23VFRR101 pKa = 11.84AAEE104 pKa = 3.86RR105 pKa = 3.64

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2492

0

2492

781252

37

3937

313.5

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.024 ± 0.048

0.891 ± 0.017

6.046 ± 0.052

6.296 ± 0.044

3.924 ± 0.033

6.859 ± 0.056

2.422 ± 0.027

6.272 ± 0.037

4.757 ± 0.047

10.504 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.023

3.959 ± 0.035

4.031 ± 0.03

4.814 ± 0.043

4.969 ± 0.047

6.141 ± 0.042

5.466 ± 0.044

6.864 ± 0.037

1.284 ± 0.021

2.796 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski