uncultured archaeon A07HR60

Taxonomy: cellular organisms; Archaea; environmental samples

Average proteome isoelectric point is 5.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4Y7K7|V4Y7K7_9ARCH GTP-binding protein HflX OS=uncultured archaeon A07HR60 OX=1412874 GN=A07HR60_02000 PE=4 SV=1
MM1 pKa = 8.31LEE3 pKa = 4.39IGQSAPDD10 pKa = 3.41FTLPNQHH17 pKa = 6.34GEE19 pKa = 4.24QVSLSAHH26 pKa = 6.62DD27 pKa = 4.12GFAVVYY33 pKa = 6.83FFPRR37 pKa = 11.84ADD39 pKa = 3.73TPGCTAEE46 pKa = 4.11ACSFRR51 pKa = 11.84DD52 pKa = 3.03KK53 pKa = 10.7WEE55 pKa = 4.12SFAEE59 pKa = 3.86RR60 pKa = 11.84DD61 pKa = 3.43VSVFGISDD69 pKa = 3.99DD70 pKa = 3.96PVSDD74 pKa = 4.58LDD76 pKa = 5.41AFAADD81 pKa = 3.87YY82 pKa = 11.06DD83 pKa = 4.33LQFHH87 pKa = 6.98LLSDD91 pKa = 3.64TDD93 pKa = 4.16GDD95 pKa = 3.84VSASYY100 pKa = 11.09GSYY103 pKa = 10.74GEE105 pKa = 4.03KK106 pKa = 10.35NIYY109 pKa = 10.35GNTIEE114 pKa = 4.69GVFRR118 pKa = 11.84NSFVVDD124 pKa = 3.73PNGDD128 pKa = 3.15VALVYY133 pKa = 10.6EE134 pKa = 4.84GVDD137 pKa = 3.8PQTHH141 pKa = 5.69GQDD144 pKa = 2.96ILADD148 pKa = 3.9LAEE151 pKa = 4.65LEE153 pKa = 4.44NNN155 pKa = 3.75

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4YGJ0|V4YGJ0_9ARCH Uncharacterized protein OS=uncultured archaeon A07HR60 OX=1412874 GN=A07HR60_02412 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.62VEE4 pKa = 4.86ADD6 pKa = 3.98YY7 pKa = 10.36TCDD10 pKa = 2.76ICGEE14 pKa = 4.16SFEE17 pKa = 4.93TNVEE21 pKa = 4.08IAGHH25 pKa = 4.73VAGHH29 pKa = 6.28RR30 pKa = 11.84RR31 pKa = 11.84SITQDD36 pKa = 3.26HH37 pKa = 7.0IIEE40 pKa = 4.13EE41 pKa = 4.27LRR43 pKa = 11.84RR44 pKa = 11.84VAEE47 pKa = 3.94QKK49 pKa = 10.16GRR51 pKa = 11.84KK52 pKa = 8.46ARR54 pKa = 11.84RR55 pKa = 11.84DD56 pKa = 3.49DD57 pKa = 3.77GRR59 pKa = 11.84PSSWVRR65 pKa = 11.84RR66 pKa = 11.84GLPLQGRR73 pKa = 11.84SRR75 pKa = 11.84RR76 pKa = 11.84GPMAGATYY84 pKa = 10.39RR85 pKa = 11.84RR86 pKa = 11.84GDD88 pKa = 3.58RR89 pKa = 11.84EE90 pKa = 3.55RR91 pKa = 11.84HH92 pKa = 5.11TNHH95 pKa = 6.68GSGALRR101 pKa = 11.84NGSS104 pKa = 3.34

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2853

0

2853

738654

28

1885

258.9

28.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.344 ± 0.059

0.832 ± 0.017

8.043 ± 0.058

7.712 ± 0.06

3.133 ± 0.028

8.471 ± 0.052

2.11 ± 0.023

4.409 ± 0.031

1.914 ± 0.026

8.497 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.771 ± 0.022

2.515 ± 0.028

4.787 ± 0.032

3.307 ± 0.025

6.589 ± 0.046

6.438 ± 0.043

6.933 ± 0.046

8.483 ± 0.048

1.093 ± 0.02

2.507 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski