Wolbachia pipientis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Wolbachieae; Wolbachia

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7QJR4|A0A1E7QJR4_WOLPI Probable lipid II flippase MurJ OS=Wolbachia pipientis OX=955 GN=murJ PE=3 SV=1
MM1 pKa = 6.62STNYY5 pKa = 10.5NINLTDD11 pKa = 3.29NALRR15 pKa = 11.84KK16 pKa = 9.17IRR18 pKa = 11.84SIAEE22 pKa = 3.79QEE24 pKa = 4.12EE25 pKa = 4.72TQGSVLRR32 pKa = 11.84IAVSGGGCSGFKK44 pKa = 10.36YY45 pKa = 10.68DD46 pKa = 5.7FLIDD50 pKa = 3.73QLSSEE55 pKa = 4.27GDD57 pKa = 3.21KK58 pKa = 11.27DD59 pKa = 5.42DD60 pKa = 5.12EE61 pKa = 5.53FEE63 pKa = 5.94DD64 pKa = 6.04DD65 pKa = 5.32YY66 pKa = 11.98DD67 pKa = 6.37DD68 pKa = 5.16EE69 pKa = 7.64DD70 pKa = 5.53GDD72 pKa = 4.24EE73 pKa = 5.81DD74 pKa = 4.87GDD76 pKa = 3.8EE77 pKa = 5.67DD78 pKa = 5.39GDD80 pKa = 3.88FDD82 pKa = 5.71EE83 pKa = 7.24DD84 pKa = 5.42EE85 pKa = 5.06EE86 pKa = 6.85DD87 pKa = 6.12DD88 pKa = 5.35DD89 pKa = 6.42DD90 pKa = 7.09DD91 pKa = 4.69YY92 pKa = 12.26YY93 pKa = 11.47KK94 pKa = 11.41ANGKK98 pKa = 10.12YY99 pKa = 9.89IVISDD104 pKa = 4.06CNGNPVLMIDD114 pKa = 3.34NHH116 pKa = 5.52SAEE119 pKa = 4.11FLKK122 pKa = 10.94DD123 pKa = 3.47SVIDD127 pKa = 3.84YY128 pKa = 11.38VEE130 pKa = 4.61DD131 pKa = 3.61LSGAGFQIKK140 pKa = 10.24SEE142 pKa = 4.14IAKK145 pKa = 8.39SHH147 pKa = 6.34CGCGNSFAII156 pKa = 4.89

Molecular weight: 17.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7QJ05|A0A1E7QJ05_WOLPI Holo-[acyl-carrier-protein] synthase OS=Wolbachia pipientis OX=955 GN=acpS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.99NLIRR12 pKa = 11.84KK13 pKa = 8.2RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.34GFRR19 pKa = 11.84TRR21 pKa = 11.84MATRR25 pKa = 11.84AGRR28 pKa = 11.84KK29 pKa = 7.83ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SLGCRR40 pKa = 11.84KK41 pKa = 9.8LCAA44 pKa = 4.11

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

851

0

851

259824

44

1975

305.3

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.943 ± 0.07

1.517 ± 0.03

5.218 ± 0.06

6.043 ± 0.089

4.475 ± 0.067

5.516 ± 0.085

2.243 ± 0.038

10.063 ± 0.089

7.82 ± 0.082

9.826 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.037

6.248 ± 0.082

2.954 ± 0.043

3.233 ± 0.053

3.774 ± 0.052

7.143 ± 0.062

4.835 ± 0.049

6.196 ± 0.065

0.747 ± 0.022

3.886 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski