Wuhan Insect virus 7

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXP4|A0A0B5KXP4_9RHAB Putative phosphoprotein OS=Wuhan Insect virus 7 OX=1608112 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 9.48QQNYY6 pKa = 9.31IYY8 pKa = 10.33PSMEE12 pKa = 3.83LRR14 pKa = 11.84NLTGPKK20 pKa = 10.05KK21 pKa = 10.3FFASGKK27 pKa = 9.84GYY29 pKa = 8.49LTKK32 pKa = 10.24EE33 pKa = 3.9YY34 pKa = 10.09RR35 pKa = 11.84NSPFAEE41 pKa = 3.97QGQLAVATNPRR52 pKa = 11.84RR53 pKa = 11.84TSWAMSDD60 pKa = 3.43EE61 pKa = 4.24EE62 pKa = 5.54GNIKK66 pKa = 10.61DD67 pKa = 5.2LGDD70 pKa = 3.83AFQKK74 pKa = 10.55EE75 pKa = 4.7LEE77 pKa = 4.37QKK79 pKa = 10.43HH80 pKa = 6.43SSDD83 pKa = 3.76SDD85 pKa = 3.73SQTSVSSNDD94 pKa = 3.59TVKK97 pKa = 9.97MDD99 pKa = 4.99PPPPPPPEE107 pKa = 3.66EE108 pKa = 4.48DD109 pKa = 3.3MIEE112 pKa = 4.02LVLPYY117 pKa = 9.65MEE119 pKa = 5.6IDD121 pKa = 3.79LARR124 pKa = 11.84KK125 pKa = 6.68VTEE128 pKa = 5.29AIDD131 pKa = 3.9LALKK135 pKa = 10.53DD136 pKa = 4.61LIIDD140 pKa = 3.99RR141 pKa = 11.84SLPRR145 pKa = 11.84IKK147 pKa = 11.02YY148 pKa = 9.36MMVPKK153 pKa = 9.88PKK155 pKa = 9.87EE156 pKa = 3.98ATPPPPPLQPPQEE169 pKa = 4.27TSTPPMQAQPSTSMPKK185 pKa = 10.1RR186 pKa = 11.84SGPLPPTEE194 pKa = 4.01STSRR198 pKa = 11.84EE199 pKa = 3.94RR200 pKa = 11.84EE201 pKa = 3.49RR202 pKa = 11.84SALVVEE208 pKa = 5.06FMKK211 pKa = 10.78GVKK214 pKa = 9.74LVGIYY219 pKa = 10.13EE220 pKa = 4.15PEE222 pKa = 3.49QTYY225 pKa = 7.7TLKK228 pKa = 10.59IGVGGVTEE236 pKa = 4.46DD237 pKa = 5.16LINEE241 pKa = 4.07VNEE244 pKa = 4.15QGLDD248 pKa = 3.32QPTQEE253 pKa = 4.62FIEE256 pKa = 4.26NLLCRR261 pKa = 11.84LEE263 pKa = 4.18YY264 pKa = 10.83GQTLHH269 pKa = 6.94SLYY272 pKa = 10.81EE273 pKa = 3.98LAA275 pKa = 5.75

Molecular weight: 30.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KXP4|A0A0B5KXP4_9RHAB Putative phosphoprotein OS=Wuhan Insect virus 7 OX=1608112 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.3KK4 pKa = 10.33QFFKK8 pKa = 11.21VGDD11 pKa = 3.62LTRR14 pKa = 11.84TQVTLLAPHH23 pKa = 6.44PQVPAQYY30 pKa = 8.94PSEE33 pKa = 4.11YY34 pKa = 10.36FVNNKK39 pKa = 9.28NKK41 pKa = 9.65PKK43 pKa = 10.56VVLSVKK49 pKa = 10.1GLNVEE54 pKa = 4.46NIRR57 pKa = 11.84AVINSGLSNNNLRR70 pKa = 11.84LAPSLYY76 pKa = 10.46FIYY79 pKa = 10.18LYY81 pKa = 10.37FLNVKK86 pKa = 8.79TVLTKK91 pKa = 9.77KK92 pKa = 9.62WEE94 pKa = 4.11SFGIKK99 pKa = 10.12FGDD102 pKa = 3.75PNEE105 pKa = 4.42EE106 pKa = 3.83VTPWSLIDD114 pKa = 3.59VQLDD118 pKa = 3.33SDD120 pKa = 4.33SGVDD124 pKa = 3.53HH125 pKa = 7.48PSLEE129 pKa = 4.89GIAKK133 pKa = 10.19SDD135 pKa = 4.19DD136 pKa = 2.97NWIICLLLSMHH147 pKa = 6.57RR148 pKa = 11.84LKK150 pKa = 11.0SGMLDD155 pKa = 3.37SYY157 pKa = 11.54RR158 pKa = 11.84EE159 pKa = 4.09IIQKK163 pKa = 10.29RR164 pKa = 11.84IYY166 pKa = 10.66DD167 pKa = 3.37QMHH170 pKa = 5.34QYY172 pKa = 10.76GYY174 pKa = 10.96KK175 pKa = 9.99KK176 pKa = 10.42GGYY179 pKa = 8.01PSSFTSEE186 pKa = 3.82DD187 pKa = 3.07WAGSLDD193 pKa = 3.84FTRR196 pKa = 11.84MVAAIDD202 pKa = 3.57MFLSKK207 pKa = 10.62FPTLVQSQLRR217 pKa = 11.84ICTLTSRR224 pKa = 11.84FKK226 pKa = 11.16DD227 pKa = 3.5CTALMSLGFIKK238 pKa = 10.42EE239 pKa = 4.23VLCLPDD245 pKa = 3.4EE246 pKa = 4.67SEE248 pKa = 4.02FLDD251 pKa = 4.25WIFTAPIAAEE261 pKa = 4.01AEE263 pKa = 3.83AMARR267 pKa = 11.84HH268 pKa = 5.25GQEE271 pKa = 3.71TLEE274 pKa = 4.37PDD276 pKa = 3.49SYY278 pKa = 11.48FPYY281 pKa = 10.57QSDD284 pKa = 3.27LRR286 pKa = 11.84LVNRR290 pKa = 11.84THH292 pKa = 6.66YY293 pKa = 10.46SASANEE299 pKa = 4.38NIFFLIHH306 pKa = 7.01AIGSLLHH313 pKa = 5.67VQRR316 pKa = 11.84SMNAKK321 pKa = 8.5KK322 pKa = 7.45TTDD325 pKa = 3.07KK326 pKa = 11.44NIINNSCNAKK336 pKa = 9.71IIAYY340 pKa = 8.85VFSNTFKK347 pKa = 10.37MAKK350 pKa = 8.99QYY352 pKa = 10.77QAIGGPAVQKK362 pKa = 10.87RR363 pKa = 11.84NLNINKK369 pKa = 9.56PEE371 pKa = 4.22DD372 pKa = 4.2LEE374 pKa = 4.84PKK376 pKa = 8.02TQNAALWYY384 pKa = 10.08AYY386 pKa = 7.51MQSYY390 pKa = 10.48DD391 pKa = 4.19FKK393 pKa = 10.34LTPNMRR399 pKa = 11.84AFVEE403 pKa = 4.29RR404 pKa = 11.84SKK406 pKa = 11.8AEE408 pKa = 3.69MGEE411 pKa = 4.24TRR413 pKa = 11.84EE414 pKa = 4.28GTIGHH419 pKa = 5.76YY420 pKa = 8.88VQHH423 pKa = 6.7HH424 pKa = 5.56FF425 pKa = 4.63

Molecular weight: 48.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3554

212

2120

710.8

81.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.361 ± 0.562

1.716 ± 0.48

5.29 ± 0.099

5.881 ± 0.647

4.221 ± 0.35

5.346 ± 0.142

2.673 ± 0.461

6.669 ± 0.425

6.021 ± 0.269

10.298 ± 0.686

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.011 ± 0.221

4.699 ± 0.336

5.627 ± 0.965

3.658 ± 0.416

5.093 ± 0.473

8.666 ± 0.245

5.515 ± 0.159

5.824 ± 0.76

1.66 ± 0.225

3.77 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski