Chenuda virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0H4MKD1|A0A0H4MKD1_9REOV Core protein VP7 OS=Chenuda virus OX=40065 GN=VP7 PE=3 SV=1
MM1 pKa = 7.89DD2 pKa = 5.84AYY4 pKa = 10.62NARR7 pKa = 11.84ALSVLEE13 pKa = 4.05GLALAADD20 pKa = 4.33PRR22 pKa = 11.84AHH24 pKa = 7.34RR25 pKa = 11.84DD26 pKa = 3.47PVSEE30 pKa = 4.18TTLSIFMMRR39 pKa = 11.84FNSTTTRR46 pKa = 11.84PIVGAPTTRR55 pKa = 11.84EE56 pKa = 3.42ARR58 pKa = 11.84RR59 pKa = 11.84NNFYY63 pKa = 10.95AALDD67 pKa = 3.52VAYY70 pKa = 9.41AALGITSQFLMPGYY84 pKa = 9.49VQNPQTLAILARR96 pKa = 11.84DD97 pKa = 4.53EE98 pKa = 4.44IPYY101 pKa = 9.29TPSTFRR107 pKa = 11.84RR108 pKa = 11.84VQRR111 pKa = 11.84IRR113 pKa = 11.84ICSEE117 pKa = 3.82GACTFRR123 pKa = 11.84EE124 pKa = 4.54EE125 pKa = 4.06YY126 pKa = 10.43FPYY129 pKa = 10.43QNYY132 pKa = 8.5TMLTGRR138 pKa = 11.84AMPINPPADD147 pKa = 3.9GGPRR151 pKa = 11.84CYY153 pKa = 10.45LVDD156 pKa = 4.16PNTVQVHH163 pKa = 5.21VEE165 pKa = 4.04PEE167 pKa = 3.59QTIAVTDD174 pKa = 3.86VVVPTAPNWVAAEE187 pKa = 4.07LAWYY191 pKa = 8.89IQTNGPGANAAEE203 pKa = 5.69GYY205 pKa = 10.1AQDD208 pKa = 2.92VDD210 pKa = 4.72VYY212 pKa = 11.37VNDD215 pKa = 3.47RR216 pKa = 11.84RR217 pKa = 11.84LPAGLPYY224 pKa = 10.58RR225 pKa = 11.84LNPGDD230 pKa = 4.0RR231 pKa = 11.84VEE233 pKa = 4.62LTNHH237 pKa = 7.21DD238 pKa = 4.35DD239 pKa = 4.1FNLGTAMFFIRR250 pKa = 11.84RR251 pKa = 11.84YY252 pKa = 8.65WSAAPPQVIYY262 pKa = 11.04DD263 pKa = 3.72SMEE266 pKa = 4.65ADD268 pKa = 2.79ICAVYY273 pKa = 9.93IYY275 pKa = 10.46HH276 pKa = 7.49DD277 pKa = 4.18RR278 pKa = 11.84TWHH281 pKa = 6.4RR282 pKa = 11.84LRR284 pKa = 11.84SYY286 pKa = 10.44ICTQVGLPPTYY297 pKa = 10.08YY298 pKa = 9.9PSEE301 pKa = 4.07ATQEE305 pKa = 3.94PRR307 pKa = 11.84RR308 pKa = 11.84VLTIAILSRR317 pKa = 11.84LFDD320 pKa = 3.73VYY322 pKa = 10.84CALSPEE328 pKa = 3.97IHH330 pKa = 6.56LPAPEE335 pKa = 4.14VAPGDD340 pKa = 3.39LAQRR344 pKa = 11.84LRR346 pKa = 11.84EE347 pKa = 4.04ALQTLRR353 pKa = 11.84GAPPPAAPRR362 pKa = 11.84GGQQ365 pKa = 2.99

Molecular weight:
40.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0H4M927|A0A0H4M927_9REOV Outer capsid protein OS=Chenuda virus OX=40065 GN=VP4 PE=4 SV=1
MM1 pKa = 7.53LSVADD6 pKa = 3.96AKK8 pKa = 10.7RR9 pKa = 11.84RR10 pKa = 11.84EE11 pKa = 4.16QMAGTEE17 pKa = 4.07MRR19 pKa = 11.84PLVPSAPRR27 pKa = 11.84PLPGAMPEE35 pKa = 3.94MALGVLQNALTSSTGANEE53 pKa = 3.75TAKK56 pKa = 10.74NEE58 pKa = 3.88KK59 pKa = 9.6AAYY62 pKa = 8.52GAASEE67 pKa = 4.67VMRR70 pKa = 11.84DD71 pKa = 3.74DD72 pKa = 3.95PATRR76 pKa = 11.84QLKK79 pKa = 8.69VHH81 pKa = 6.07VSVTAIAEE89 pKa = 3.95LEE91 pKa = 3.93EE92 pKa = 4.28RR93 pKa = 11.84YY94 pKa = 10.29KK95 pKa = 10.36RR96 pKa = 11.84VRR98 pKa = 11.84RR99 pKa = 11.84KK100 pKa = 9.97KK101 pKa = 10.23CACKK105 pKa = 9.17WLQAISSALVLTTSIAMSIVTASTWVEE132 pKa = 3.58NTLVKK137 pKa = 10.81NGVPVYY143 pKa = 9.47MIISGLTTVAMVAGRR158 pKa = 11.84MHH160 pKa = 6.59AHH162 pKa = 6.58ARR164 pKa = 11.84TATRR168 pKa = 11.84TIKK171 pKa = 10.37RR172 pKa = 11.84DD173 pKa = 3.41LVKK176 pKa = 10.6KK177 pKa = 10.53KK178 pKa = 10.71SYY180 pKa = 10.02VQLAVAMGTPLPGTVAAPAKK200 pKa = 10.36AGTSVGAASIVDD212 pKa = 3.75DD213 pKa = 4.33LVARR217 pKa = 11.84GWQPLPP223 pKa = 3.77

Molecular weight:
23.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

6145

183

1285

512.1

57.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.301 ± 0.604

1.383 ± 0.242

5.435 ± 0.341

5.891 ± 0.249

3.466 ± 0.333

5.696 ± 0.395

2.571 ± 0.226

5.028 ± 0.275

2.799 ± 0.503

9.552 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.264

2.587 ± 0.212

6.151 ± 0.427

3.531 ± 0.417

9.39 ± 0.431

5.663 ± 0.391

6.363 ± 0.37

7.681 ± 0.324

1.155 ± 0.187

2.897 ± 0.276

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski