Gordonia phage GMA5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0MWM8|A0A0K0MWM8_9CAUD Uncharacterized protein OS=Gordonia phage GMA5 OX=1647472 GN=GMA5_13 PE=4 SV=1
MM1 pKa = 7.8ASTLALALDD10 pKa = 3.95RR11 pKa = 11.84VVEE14 pKa = 4.03ALTEE18 pKa = 3.99VGIAATTDD26 pKa = 3.3PRR28 pKa = 11.84DD29 pKa = 3.66LQIPGAWVTVHH40 pKa = 7.18DD41 pKa = 5.16VIDD44 pKa = 3.82PTLCGSFTVRR54 pKa = 11.84ADD56 pKa = 3.22VCLIAADD63 pKa = 3.47NGMPFEE69 pKa = 4.25VEE71 pKa = 3.91ALGTLLDD78 pKa = 4.23LVADD82 pKa = 4.12AVTFDD87 pKa = 3.56EE88 pKa = 5.0PVRR91 pKa = 11.84PMTVTPPGLAPLPALVITTTTEE113 pKa = 3.43

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0MWF0|A0A0K0MWF0_9CAUD Uncharacterized protein OS=Gordonia phage GMA5 OX=1647472 GN=GMA5_22 PE=4 SV=1
MM1 pKa = 7.46SIWSAAIAAFISHH14 pKa = 7.28LAAGGRR20 pKa = 11.84SVGTRR25 pKa = 11.84QLRR28 pKa = 11.84RR29 pKa = 11.84YY30 pKa = 8.61HH31 pKa = 6.92LSRR34 pKa = 11.84FAEE37 pKa = 4.39VVDD40 pKa = 3.92PLGPLDD46 pKa = 3.81VDD48 pKa = 4.53VVDD51 pKa = 4.85LEE53 pKa = 4.31AWLGRR58 pKa = 11.84DD59 pKa = 2.96DD60 pKa = 3.28WTANTRR66 pKa = 11.84RR67 pKa = 11.84SARR70 pKa = 11.84SSLVTFFSWIAANGWRR86 pKa = 11.84ADD88 pKa = 3.57NPAALLATVTGVLGRR103 pKa = 11.84PRR105 pKa = 11.84PCPEE109 pKa = 3.23QHH111 pKa = 6.23LRR113 pKa = 11.84AAVVAAGPRR122 pKa = 11.84EE123 pKa = 4.03RR124 pKa = 11.84LMLALGAGCGLRR136 pKa = 11.84RR137 pKa = 11.84AEE139 pKa = 3.79ISKK142 pKa = 10.12VCGEE146 pKa = 4.25HH147 pKa = 7.64VEE149 pKa = 4.22DD150 pKa = 4.74TMDD153 pKa = 3.76GPILRR158 pKa = 11.84VVGKK162 pKa = 9.45GGKK165 pKa = 8.65VRR167 pKa = 11.84EE168 pKa = 4.13VPISDD173 pKa = 3.84DD174 pKa = 3.33LAVRR178 pKa = 11.84LRR180 pKa = 11.84EE181 pKa = 3.8RR182 pKa = 11.84AGYY185 pKa = 10.08AFPSPRR191 pKa = 11.84GGHH194 pKa = 5.94LTPAHH199 pKa = 5.76VGKK202 pKa = 10.26LVSRR206 pKa = 11.84LLPPGWTTHH215 pKa = 4.72TLRR218 pKa = 11.84HH219 pKa = 5.89RR220 pKa = 11.84FASAAYY226 pKa = 9.79RR227 pKa = 11.84ADD229 pKa = 3.86RR230 pKa = 11.84DD231 pKa = 3.24IRR233 pKa = 11.84AVQEE237 pKa = 4.27LLGHH241 pKa = 6.66ASVATTQIYY250 pKa = 7.84TAIPDD255 pKa = 3.83DD256 pKa = 3.59ATRR259 pKa = 11.84RR260 pKa = 11.84ASLAASIVAA269 pKa = 4.3

Molecular weight: 29.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

5508

34

675

196.7

21.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.943 ± 0.927

0.853 ± 0.171

6.245 ± 0.61

4.085 ± 0.376

2.633 ± 0.221

7.97 ± 0.481

2.215 ± 0.372

5.265 ± 0.655

2.288 ± 0.214

7.662 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.17

2.542 ± 0.377

5.955 ± 0.439

2.687 ± 0.191

7.262 ± 0.712

6.009 ± 0.278

7.643 ± 0.369

8.297 ± 0.474

2.197 ± 0.202

2.088 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski