Mesorhizobium amorphae CCBAU 01583

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; Mesorhizobium amorphae

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4266 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A212DJJ8|A0A212DJJ8_9RHIZ Acetyltransferase OS=Mesorhizobium amorphae CCBAU 01583 OX=1449348 GN=AJ88_05185 PE=4 SV=1
MM1 pKa = 7.57SMKK4 pKa = 10.54KK5 pKa = 10.43LILGLSLSLLASSAFAADD23 pKa = 3.3AVVQEE28 pKa = 4.81GVDD31 pKa = 3.71VAPVFSWTGGYY42 pKa = 9.53IGLQAGYY49 pKa = 10.36AWGDD53 pKa = 3.6GNIDD57 pKa = 3.75QIDD60 pKa = 4.03GPGFVEE66 pKa = 4.17TDD68 pKa = 3.06PDD70 pKa = 3.86GFLGGVYY77 pKa = 10.19AGYY80 pKa = 10.37NYY82 pKa = 10.35QMSSNIVIGAEE93 pKa = 3.73LDD95 pKa = 3.84FVFAGIDD102 pKa = 3.39GSGQIFTAPGVPFPGAIATEE122 pKa = 4.04EE123 pKa = 4.41LNWSGAARR131 pKa = 11.84LRR133 pKa = 11.84LGYY136 pKa = 10.56AVDD139 pKa = 3.97RR140 pKa = 11.84FLPYY144 pKa = 9.91IAGGVAFGDD153 pKa = 3.47IDD155 pKa = 3.38ISTNSGAPSFGDD167 pKa = 3.54TFTGWTIGVGLDD179 pKa = 3.46YY180 pKa = 11.81AMTDD184 pKa = 3.1NLLLRR189 pKa = 11.84TEE191 pKa = 3.97YY192 pKa = 10.46RR193 pKa = 11.84YY194 pKa = 8.57TDD196 pKa = 3.85FGSEE200 pKa = 4.35SFGPPANADD209 pKa = 2.98VDD211 pKa = 4.55LKK213 pKa = 9.97TNEE216 pKa = 3.65VRR218 pKa = 11.84FGIAYY223 pKa = 9.45KK224 pKa = 10.53FF225 pKa = 3.41

Molecular weight: 23.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A212DV79|A0A212DV79_9RHIZ Uncharacterized protein OS=Mesorhizobium amorphae CCBAU 01583 OX=1449348 GN=AJ88_15440 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84GKK4 pKa = 10.71LLLVRR9 pKa = 11.84NGTKK13 pKa = 9.77RR14 pKa = 11.84VPWGRR19 pKa = 11.84STKK22 pKa = 9.89EE23 pKa = 3.64RR24 pKa = 11.84LKK26 pKa = 11.17SPTRR30 pKa = 11.84PISFGSTKK38 pKa = 9.91IAPASIHH45 pKa = 5.01TFIGIFKK52 pKa = 9.72WGTKK56 pKa = 9.43PLIGGMRR63 pKa = 11.84PRR65 pKa = 11.84LFSIAIKK72 pKa = 10.39LRR74 pKa = 11.84RR75 pKa = 3.6

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4266

0

4266

654768

41

796

153.5

16.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.034 ± 0.067

1.303 ± 0.019

5.592 ± 0.036

5.335 ± 0.036

3.579 ± 0.029

7.992 ± 0.044

2.023 ± 0.02

4.994 ± 0.031

3.601 ± 0.033

9.116 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.02

2.642 ± 0.024

5.435 ± 0.04

3.196 ± 0.028

8.342 ± 0.05

6.71 ± 0.05

5.442 ± 0.038

6.696 ± 0.035

1.42 ± 0.02

1.953 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski