Candidatus Nitrosoglobus terrae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Candidatus Nitrosoglobus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q2SK62|A0A1Q2SK62_9GAMM General secretion pathway protein D OS=Candidatus Nitrosoglobus terrae OX=1630141 GN=TAO_0161 PE=3 SV=1
MM1 pKa = 6.9NTIDD5 pKa = 3.84TTPVLLTFTDD15 pKa = 3.89AAATKK20 pKa = 9.66VKK22 pKa = 10.39EE23 pKa = 4.32LIEE26 pKa = 4.1EE27 pKa = 4.25EE28 pKa = 4.49GNKK31 pKa = 10.3NLMLRR36 pKa = 11.84VFISGGGCSGFQYY49 pKa = 11.02GFTFDD54 pKa = 3.85DD55 pKa = 4.28TLNEE59 pKa = 5.14DD60 pKa = 3.47DD61 pKa = 4.39TQVEE65 pKa = 4.43KK66 pKa = 11.15QGVTLLIDD74 pKa = 3.64PTSYY78 pKa = 10.75QYY80 pKa = 11.46LIGAEE85 pKa = 3.69IDD87 pKa = 3.59YY88 pKa = 9.87TEE90 pKa = 4.22SVEE93 pKa = 4.07GSEE96 pKa = 4.66FVIRR100 pKa = 11.84NPNATTTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q2SLP2|A0A1Q2SLP2_9GAMM 30S ribosomal protein S14 OS=Candidatus Nitrosoglobus terrae OX=1630141 GN=rpsN PE=3 SV=1
MM1 pKa = 7.51SKK3 pKa = 9.11TGGRR7 pKa = 11.84NNQGRR12 pKa = 11.84ITVRR16 pKa = 11.84HH17 pKa = 6.08RR18 pKa = 11.84GGGHH22 pKa = 5.29KK23 pKa = 10.01RR24 pKa = 11.84LYY26 pKa = 10.37RR27 pKa = 11.84VIDD30 pKa = 3.73FNRR33 pKa = 11.84DD34 pKa = 3.13KK35 pKa = 10.9IDD37 pKa = 2.91ISGYY41 pKa = 7.19VEE43 pKa = 3.85RR44 pKa = 11.84LEE46 pKa = 4.07YY47 pKa = 10.81DD48 pKa = 3.74PNRR51 pKa = 11.84SAHH54 pKa = 6.35IALISYY60 pKa = 10.71SDD62 pKa = 3.58GEE64 pKa = 4.07KK65 pKa = 10.3RR66 pKa = 11.84YY67 pKa = 10.13IIAPKK72 pKa = 8.11TIKK75 pKa = 10.14MGDD78 pKa = 3.19IVVSGQEE85 pKa = 3.75VPIRR89 pKa = 11.84DD90 pKa = 4.28GNCLPLRR97 pKa = 11.84NIPIGALIHH106 pKa = 6.6CIEE109 pKa = 4.7LKK111 pKa = 9.87PGKK114 pKa = 9.72GAQLARR120 pKa = 11.84SAGASCQLVARR131 pKa = 11.84EE132 pKa = 4.13DD133 pKa = 3.89RR134 pKa = 11.84YY135 pKa = 9.37VTLRR139 pKa = 11.84LRR141 pKa = 11.84SGEE144 pKa = 3.91MRR146 pKa = 11.84KK147 pKa = 9.36VLPEE151 pKa = 3.69CRR153 pKa = 11.84ATIGEE158 pKa = 4.32VGNDD162 pKa = 3.31EE163 pKa = 5.69HH164 pKa = 6.97GLCSLGKK171 pKa = 10.23AGVKK175 pKa = 9.31RR176 pKa = 11.84WQGKK180 pKa = 8.39RR181 pKa = 11.84PTVRR185 pKa = 11.84GVAMNPVDD193 pKa = 3.85HH194 pKa = 6.82PHH196 pKa = 6.7GGGEE200 pKa = 4.02GRR202 pKa = 11.84TSGGRR207 pKa = 11.84HH208 pKa = 5.5PVSPWGIPTKK218 pKa = 10.48GYY220 pKa = 7.91KK221 pKa = 8.43TRR223 pKa = 11.84NNKK226 pKa = 8.18RR227 pKa = 11.84TEE229 pKa = 3.71NLIARR234 pKa = 11.84RR235 pKa = 11.84RR236 pKa = 11.84KK237 pKa = 9.71NKK239 pKa = 10.05

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1841

0

1841

585648

43

1842

318.1

35.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.506 ± 0.057

1.015 ± 0.019

4.734 ± 0.031

5.965 ± 0.057

3.963 ± 0.038

7.156 ± 0.054

2.386 ± 0.025

7.432 ± 0.045

4.787 ± 0.049

11.086 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.023

3.941 ± 0.042

4.466 ± 0.036

4.714 ± 0.046

5.587 ± 0.049

6.036 ± 0.036

5.295 ± 0.04

6.196 ± 0.046

1.336 ± 0.024

3.243 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski