Paracoccus thiocyanatus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae;

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6Z2F9|A0A1N6Z2F9_9RHOB Amino acid ABC transporter membrane protein 2 PAAT family OS=Paracoccus thiocyanatus OX=34006 GN=DIE28_09365 PE=3 SV=1
MM1 pKa = 6.92STEE4 pKa = 3.97NLKK7 pKa = 11.09EE8 pKa = 4.13GDD10 pKa = 3.88PLIAPSTTDD19 pKa = 2.82HH20 pKa = 7.15PLYY23 pKa = 10.78DD24 pKa = 4.23SVVEE28 pKa = 4.16ACKK31 pKa = 9.47TVYY34 pKa = 10.67DD35 pKa = 4.01PEE37 pKa = 5.23IPVNIYY43 pKa = 10.8DD44 pKa = 4.42LGLIYY49 pKa = 10.34TIDD52 pKa = 3.49ISDD55 pKa = 3.73EE56 pKa = 3.94NAVRR60 pKa = 11.84IVMTLTAPGCPVAGEE75 pKa = 4.15MPGWVADD82 pKa = 3.91AVEE85 pKa = 4.55PLPGVKK91 pKa = 9.77QVDD94 pKa = 3.57VEE96 pKa = 4.6MTFQPQWGMDD106 pKa = 3.61MMSEE110 pKa = 4.19EE111 pKa = 4.48ARR113 pKa = 11.84LEE115 pKa = 4.05LGFII119 pKa = 4.34

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6SXS0|A0A1N6SXS0_9RHOB Uncharacterized protein OS=Paracoccus thiocyanatus OX=34006 GN=DIE28_16940 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84ARR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.2AGRR29 pKa = 11.84RR30 pKa = 11.84VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.0GRR40 pKa = 11.84KK41 pKa = 6.51TLSAA45 pKa = 4.15

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3285

0

3285

956036

27

2093

291.0

31.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.42 ± 0.063

0.855 ± 0.014

5.799 ± 0.041

5.473 ± 0.038

3.509 ± 0.024

9.135 ± 0.039

2.099 ± 0.017

4.995 ± 0.029

2.601 ± 0.034

10.384 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.789 ± 0.02

2.253 ± 0.021

5.521 ± 0.034

3.455 ± 0.026

7.642 ± 0.042

4.693 ± 0.028

4.827 ± 0.029

7.038 ± 0.035

1.474 ± 0.018

2.038 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski