Peptoniphilus sp. oral taxon 375 str. F0436

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; unclassified Peptoniphilus; Peptoniphilus sp. oral taxon 375

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9MUB4|F9MUB4_9FIRM Uncharacterized protein OS=Peptoniphilus sp. oral taxon 375 str. F0436 OX=879308 GN=HMPREF9130_2195 PE=4 SV=1
MM1 pKa = 8.0KK2 pKa = 10.17YY3 pKa = 10.43QSNQFSQKK11 pKa = 10.4YY12 pKa = 8.5EE13 pKa = 3.91DD14 pKa = 3.67ASFEE18 pKa = 4.02IFLTSSYY25 pKa = 11.54DD26 pKa = 3.44NDD28 pKa = 3.5IVEE31 pKa = 4.25GDD33 pKa = 3.62IDD35 pKa = 4.17FFDD38 pKa = 4.94YY39 pKa = 10.42IYY41 pKa = 10.91KK42 pKa = 10.54DD43 pKa = 3.5DD44 pKa = 4.04MQIKK48 pKa = 8.81TII50 pKa = 3.91

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9MS03|F9MS03_9FIRM R3H domain protein OS=Peptoniphilus sp. oral taxon 375 str. F0436 OX=879308 GN=HMPREF9130_0511 PE=4 SV=1
MM1 pKa = 6.78MRR3 pKa = 11.84KK4 pKa = 8.97FRR6 pKa = 11.84GFSSLDD12 pKa = 3.19RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84EE16 pKa = 3.71EE17 pKa = 5.05AMKK20 pKa = 10.64LWIHH24 pKa = 5.83RR25 pKa = 11.84LKK27 pKa = 9.76ITFFKK32 pKa = 10.92NRR34 pKa = 11.84IRR36 pKa = 11.84LRR38 pKa = 11.84RR39 pKa = 11.84GILRR43 pKa = 11.84TKK45 pKa = 10.51KK46 pKa = 10.22SLSPHH51 pKa = 5.35RR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 9.59GHH56 pKa = 4.59KK57 pKa = 9.37VKK59 pKa = 10.71RR60 pKa = 11.84ATKK63 pKa = 8.82VLLRR67 pKa = 11.84ALMKK71 pKa = 10.67

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2117

0

2117

563660

37

2376

266.3

30.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.392 ± 0.057

0.945 ± 0.02

6.365 ± 0.048

7.23 ± 0.064

4.257 ± 0.041

6.958 ± 0.052

1.687 ± 0.026

7.379 ± 0.049

8.169 ± 0.058

10.408 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.03

4.327 ± 0.049

3.433 ± 0.037

3.966 ± 0.048

4.193 ± 0.048

5.885 ± 0.046

4.701 ± 0.053

6.308 ± 0.049

0.781 ± 0.017

4.004 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski