Entamoeba invadens IP1

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae; Entamoeba; Entamoeba invadens

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1U0A3|A0A0A1U0A3_ENTIV Uncharacterized protein OS=Entamoeba invadens IP1 OX=370355 GN=EIN_135000 PE=4 SV=1
MM1 pKa = 7.25QNTIQFYY8 pKa = 9.55TSPAYY13 pKa = 9.18PQSLPCYY20 pKa = 8.84FQAPTLAMPNRR31 pKa = 11.84LEE33 pKa = 4.25TPFVNHH39 pKa = 6.43SFSQSQFMFAEE50 pKa = 4.08QQLPQQVVATPSVPMEE66 pKa = 4.28EE67 pKa = 4.21EE68 pKa = 4.38DD69 pKa = 3.88PFEE72 pKa = 5.24NDD74 pKa = 3.35AVFDD78 pKa = 4.28LDD80 pKa = 3.79EE81 pKa = 5.67DD82 pKa = 4.39LEE84 pKa = 4.18EE85 pKa = 6.93DD86 pKa = 3.83YY87 pKa = 11.6DD88 pKa = 3.97DD89 pKa = 3.96TNSYY93 pKa = 11.03DD94 pKa = 3.48EE95 pKa = 4.56SEE97 pKa = 4.32RR98 pKa = 11.84LADD101 pKa = 4.2QLLQTVDD108 pKa = 4.0NIRR111 pKa = 11.84QQKK114 pKa = 9.91DD115 pKa = 2.93QTNQMLRR122 pKa = 11.84QIVQMNSTHH131 pKa = 6.23

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1UBA8|A0A0A1UBA8_ENTIV Uncharacterized protein OS=Entamoeba invadens IP1 OX=370355 GN=EIN_523640 PE=4 SV=1
MM1 pKa = 8.47DD2 pKa = 4.46YY3 pKa = 11.54GCFKK7 pKa = 8.9TTLFKK12 pKa = 10.63IIPVLILSTFSYY24 pKa = 10.19IRR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.16SLRR31 pKa = 11.84RR32 pKa = 11.84IQSLILIYY40 pKa = 10.12MLLSLLFFRR49 pKa = 11.84FSQFIVSLIIFITTQSIPNMKK70 pKa = 9.89NDD72 pKa = 3.11VPIIVSSPSRR82 pKa = 11.84LTEE85 pKa = 4.5EE86 pKa = 3.58ISSRR90 pKa = 11.84ITLKK94 pKa = 10.52PFRR97 pKa = 11.84LPKK100 pKa = 10.46SLGNCRR106 pKa = 11.84VIRR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84GICNGLAPSTKK122 pKa = 10.14SSTRR126 pKa = 11.84RR127 pKa = 11.84SNRR130 pKa = 11.84LNLSWTCWGVSRR142 pKa = 11.84VVCRR146 pKa = 11.84TLRR149 pKa = 11.84LLRR152 pKa = 11.84EE153 pKa = 3.99QARR156 pKa = 11.84VPRR159 pKa = 11.84LCYY162 pKa = 10.2NEE164 pKa = 4.49SWVCGDD170 pKa = 4.94DD171 pKa = 3.43IKK173 pKa = 11.44DD174 pKa = 3.27VAFIHH179 pKa = 6.59FDD181 pKa = 3.18QLRR184 pKa = 11.84RR185 pKa = 11.84CHH187 pKa = 6.31FCII190 pKa = 5.1

Molecular weight: 22.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9857

0

9857

4270709

8

5004

433.3

49.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.087 ± 0.02

3.465 ± 0.047

5.371 ± 0.014

7.132 ± 0.035

5.177 ± 0.021

4.626 ± 0.02

1.628 ± 0.007

6.96 ± 0.019

8.552 ± 0.033

8.415 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.009

6.378 ± 0.027

3.346 ± 0.017

3.693 ± 0.019

3.34 ± 0.017

7.397 ± 0.023

6.635 ± 0.023

6.849 ± 0.019

0.692 ± 0.006

3.822 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski