Acidisphaera rubrifaciens HS-AP3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidisphaera; Acidisphaera rubrifaciens

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6P945|A0A0D6P945_9PROT Transcriptional regulator polyhydroxyalkanoate biosynthesis OS=Acidisphaera rubrifaciens HS-AP3 OX=1231350 GN=Asru_0493_03 PE=4 SV=1
MM1 pKa = 7.5ANAVTYY7 pKa = 10.31LPSFPQPTSPNQIVGLILQNNSGTATPAQNLTFGEE42 pKa = 4.46VFAAGAVASGANLVAQIGGSATPVQMDD69 pKa = 3.63VKK71 pKa = 10.02STYY74 pKa = 10.96ADD76 pKa = 3.36GSVEE80 pKa = 4.27SAVLTVAAPTLAANSSTPVMLSLGGSAPAPAVNLAGALSNYY121 pKa = 10.21SLTAALMVNGTAYY134 pKa = 10.98NIDD137 pKa = 4.11LTSAMQTALANGTANYY153 pKa = 8.12WLKK156 pKa = 11.24GPDD159 pKa = 3.02ATQARR164 pKa = 11.84VSVPVAGSLRR174 pKa = 11.84LVADD178 pKa = 4.07ITANADD184 pKa = 3.36GTFSATVGFNNDD196 pKa = 2.56VAEE199 pKa = 4.22TANGGTIALNAKK211 pKa = 9.75LIQNGSTVYY220 pKa = 10.64SSGNITQYY228 pKa = 10.94QYY230 pKa = 11.38QDD232 pKa = 2.52WSATVGTAPASTINVQHH249 pKa = 7.49DD250 pKa = 2.88IGYY253 pKa = 9.83LEE255 pKa = 4.32KK256 pKa = 10.26TGAIPTYY263 pKa = 10.52DD264 pKa = 3.8LSTGVATSVLTGDD277 pKa = 4.06ASAVASPGWNAPLAPNGINQHH298 pKa = 5.58MPTTGGRR305 pKa = 11.84GDD307 pKa = 3.85IGPTTQANADD317 pKa = 3.51WLQTQDD323 pKa = 3.38QSAMQYY329 pKa = 11.81ALGQAGNGASSVPWHH344 pKa = 6.47FWNPTAGTWLNTASYY359 pKa = 8.36PQLWNDD365 pKa = 3.19PRR367 pKa = 11.84GGNGAPGGLTQQVDD381 pKa = 3.56TGNTGWTVDD390 pKa = 3.72EE391 pKa = 4.3AHH393 pKa = 6.46QPTLDD398 pKa = 3.37YY399 pKa = 11.03DD400 pKa = 4.14AYY402 pKa = 11.17LLTGNRR408 pKa = 11.84TYY410 pKa = 11.44LDD412 pKa = 3.6NLNAQASNAVMADD425 pKa = 3.13WPGVRR430 pKa = 11.84DD431 pKa = 3.52EE432 pKa = 5.16GSYY435 pKa = 10.89TDD437 pKa = 3.59IVANGGDD444 pKa = 3.83QVRR447 pKa = 11.84QQAWSLRR454 pKa = 11.84EE455 pKa = 3.7IDD457 pKa = 3.33EE458 pKa = 4.28AAYY461 pKa = 10.5ANPAGSAEE469 pKa = 4.08KK470 pKa = 10.39AYY472 pKa = 8.42FTQTMNDD479 pKa = 2.83NWHH482 pKa = 6.45WLVSQIPAWTKK493 pKa = 11.16LEE495 pKa = 4.06GQAYY499 pKa = 9.17GYY501 pKa = 11.13VPGTYY506 pKa = 10.16GSTGTTMAPWQQDD519 pKa = 3.75YY520 pKa = 10.25FVSTTVEE527 pKa = 3.82AARR530 pKa = 11.84MGNADD535 pKa = 4.32ALTFLKK541 pKa = 10.27WEE543 pKa = 4.58SNFIIGRR550 pKa = 11.84FSQSQPGWNAHH561 pKa = 6.98DD562 pKa = 3.79GAAYY566 pKa = 10.74NLDD569 pKa = 3.52IGPANSPLQTWSAIEE584 pKa = 4.17SATAAAGDD592 pKa = 4.27SNGSGWAAGDD602 pKa = 3.7YY603 pKa = 10.58AQLGLEE609 pKa = 4.2SLAGIYY615 pKa = 10.34SVTGDD620 pKa = 3.68PQALAEE626 pKa = 4.21NNWLKK631 pKa = 11.13SSGAPFLDD639 pKa = 3.56TASYY643 pKa = 10.27QGLGSSPAEE652 pKa = 4.05QFNVAPTPSTLPPSGAPAPTPTPTPTPTPTPTPTPTPAPTPTPTPTPTTNPTPVPSSGHH711 pKa = 4.53VLQVGAGQTYY721 pKa = 8.1ATISQAVAASQSGDD735 pKa = 3.71TIQVQAGTYY744 pKa = 9.87TNDD747 pKa = 3.08FPNLISHH754 pKa = 7.58DD755 pKa = 4.03LTLEE759 pKa = 4.0AVGGVVHH766 pKa = 6.19MVATEE771 pKa = 3.71PVPNGKK777 pKa = 10.21AILDD781 pKa = 3.42IGAPGVSVSVTGFDD795 pKa = 3.61FSGATVSSGNGAGIRR810 pKa = 11.84YY811 pKa = 9.18EE812 pKa = 4.37GGSLTLNNDD821 pKa = 3.11SFEE824 pKa = 4.47NNQDD828 pKa = 3.54GLLGASDD835 pKa = 3.59TSGAITITNSVFSHH849 pKa = 6.39NGTGDD854 pKa = 3.34GHH856 pKa = 4.99THH858 pKa = 5.77NLYY861 pKa = 10.34VGEE864 pKa = 4.32VGTLTVTGSTLTDD877 pKa = 2.9AVVGHH882 pKa = 6.56EE883 pKa = 4.1IKK885 pKa = 10.76SRR887 pKa = 11.84ADD889 pKa = 3.15TTIIQGNTISDD900 pKa = 4.07GPTGTGSYY908 pKa = 10.59SIDD911 pKa = 3.56LPNGGKK917 pKa = 9.93AVISGNVIEE926 pKa = 5.11KK927 pKa = 10.5GPNAGNPAIISVGEE941 pKa = 3.97EE942 pKa = 3.7GGVYY946 pKa = 10.52ANSSVTLSNNTVLNDD961 pKa = 3.45NASPSVVLVKK971 pKa = 10.82NDD973 pKa = 3.42TTGPATISGTSVWNLTASEE992 pKa = 4.18MLQGPGSVSGTTLLASEE1009 pKa = 4.66PSIAAAAAPTPTPTPTPAPTPTPTPTPTPADD1040 pKa = 3.77TPIVVAPGGTLTTAAGTTNTVTMPTGDD1067 pKa = 3.33ATVVSGGTDD1076 pKa = 3.55TIYY1079 pKa = 11.04AGAGSDD1085 pKa = 3.95KK1086 pKa = 10.56ISSGVNTVTVNQGSGPLTFIGGYY1109 pKa = 9.55GGNATLNMAAGGTGANLTLYY1129 pKa = 11.14GNTTINAAGTFTANDD1144 pKa = 3.92GGSGAGADD1152 pKa = 3.55VFNITKK1158 pKa = 10.59GDD1160 pKa = 3.58VTNITITNFGDD1171 pKa = 3.56AFHH1174 pKa = 7.36LIGFSAGEE1182 pKa = 3.93AQQAVATAQHH1192 pKa = 5.99TGAGEE1197 pKa = 4.08VMSFSDD1203 pKa = 4.63GSQVTLAGWNHH1214 pKa = 6.31AMLSWFQQ1221 pKa = 3.24

Molecular weight: 123.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6P3Q0|A0A0D6P3Q0_9PROT Transcription termination factor Rho OS=Acidisphaera rubrifaciens HS-AP3 OX=1231350 GN=rho PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.09VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.24GRR39 pKa = 11.84QRR41 pKa = 11.84LSAA44 pKa = 4.04

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2788

0

2788

851939

39

2198

305.6

32.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.238 ± 0.071

0.939 ± 0.016

5.824 ± 0.039

4.711 ± 0.05

3.349 ± 0.026

9.138 ± 0.051

2.316 ± 0.022

4.564 ± 0.031

1.978 ± 0.034

10.079 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.022

2.173 ± 0.032

5.846 ± 0.038

2.807 ± 0.03

7.98 ± 0.064

4.499 ± 0.039

5.626 ± 0.05

7.777 ± 0.036

1.424 ± 0.018

2.334 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski