Sphingomonas sp. SRS2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5P9L5|A0A0F5P9L5_9SPHN Uncharacterized protein OS=Sphingomonas sp. SRS2 OX=133190 GN=WP12_16600 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 6.79DD3 pKa = 2.9IMTIEE8 pKa = 4.24STRR11 pKa = 11.84CCDD14 pKa = 6.2DD15 pKa = 3.31FALWPDD21 pKa = 4.14GSWATLGDD29 pKa = 3.73ISNGDD34 pKa = 3.83YY35 pKa = 10.18HH36 pKa = 6.75WKK38 pKa = 10.2SDD40 pKa = 3.56DD41 pKa = 3.78YY42 pKa = 11.66EE43 pKa = 5.67IISYY47 pKa = 10.92LEE49 pKa = 4.05TEE51 pKa = 4.44RR52 pKa = 11.84LTALGVYY59 pKa = 7.85EE60 pKa = 4.14

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5PFJ5|A0A0F5PFJ5_9SPHN Photosystem reaction center subunit H OS=Sphingomonas sp. SRS2 OX=133190 GN=WP12_04190 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 8.76ILAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84IKK41 pKa = 10.86LSAA44 pKa = 3.84

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4289

0

4289

1272449

32

1912

296.7

32.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.189 ± 0.053

0.778 ± 0.012

6.092 ± 0.029

5.388 ± 0.038

3.519 ± 0.024

8.793 ± 0.037

2.025 ± 0.02

5.486 ± 0.025

3.07 ± 0.033

9.909 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.018

2.478 ± 0.022

5.222 ± 0.027

3.025 ± 0.02

7.562 ± 0.04

5.339 ± 0.027

5.134 ± 0.028

6.907 ± 0.028

1.395 ± 0.015

2.227 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski