Diaporthe ampelina

Taxonomy:

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2HBB3|A0A0G2HBB3_9PEZI Putative mfs maltose OS=Diaporthe ampelina OX=1214573 GN=UCDDA912_g07560 PE=3 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84RR3 pKa = 11.84SIFSKK8 pKa = 10.55RR9 pKa = 11.84QDD11 pKa = 2.97ATTLEE16 pKa = 4.27NQQSGTSYY24 pKa = 10.56TIDD27 pKa = 3.44IEE29 pKa = 4.53IGTPPQPITLIIDD42 pKa = 3.81TGSSEE47 pKa = 4.55LWVNPTCEE55 pKa = 3.84TSGQPEE61 pKa = 4.27YY62 pKa = 11.0CEE64 pKa = 4.53SFSQFDD70 pKa = 3.73YY71 pKa = 10.12TASSTINDD79 pKa = 3.53TGYY82 pKa = 11.62SNMLAYY88 pKa = 10.18GKK90 pKa = 10.96GNVTIEE96 pKa = 4.12YY97 pKa = 8.2VTDD100 pKa = 3.62VVSIGSASITDD111 pKa = 3.51QIFGVGFEE119 pKa = 5.0SYY121 pKa = 10.53DD122 pKa = 3.34IPLGILGLAPPTDD135 pKa = 3.97SDD137 pKa = 3.83PLYY140 pKa = 10.71TYY142 pKa = 11.2VLDD145 pKa = 3.93NMVDD149 pKa = 3.05QGLIDD154 pKa = 3.71SRR156 pKa = 11.84AFSLDD161 pKa = 3.1LRR163 pKa = 11.84DD164 pKa = 3.39VDD166 pKa = 4.69SPDD169 pKa = 3.08GSVIFGGVDD178 pKa = 2.76TGKK181 pKa = 10.53FVGEE185 pKa = 3.98LQKK188 pKa = 11.2CPILDD193 pKa = 4.26PLDD196 pKa = 4.13TPSGADD202 pKa = 3.5RR203 pKa = 11.84YY204 pKa = 9.45WIYY207 pKa = 10.33LTGLGMTLPSGEE219 pKa = 4.49SGLIAEE225 pKa = 4.93GEE227 pKa = 4.28LPVFLDD233 pKa = 3.52SGGTMTRR240 pKa = 11.84LPAEE244 pKa = 4.04VFEE247 pKa = 4.79AVGSVFPGAQYY258 pKa = 11.08DD259 pKa = 4.0SEE261 pKa = 4.46SGYY264 pKa = 11.0FLVDD268 pKa = 3.43CDD270 pKa = 4.33VGDD273 pKa = 3.83SSGSVDD279 pKa = 4.43FVFGDD284 pKa = 3.64KK285 pKa = 10.61VISVAFDD292 pKa = 3.37DD293 pKa = 5.39FIWRR297 pKa = 11.84VPSVEE302 pKa = 4.19DD303 pKa = 3.51TCVLGILADD312 pKa = 4.23DD313 pKa = 4.77GEE315 pKa = 4.71FTRR318 pKa = 11.84ATTGGKK324 pKa = 8.45QRR326 pKa = 11.84EE327 pKa = 4.21ADD329 pKa = 3.44TTFAEE334 pKa = 4.65EE335 pKa = 4.33PVLGDD340 pKa = 3.31TFLRR344 pKa = 11.84AAYY347 pKa = 9.66VVYY350 pKa = 10.7DD351 pKa = 3.36QDD353 pKa = 4.09NRR355 pKa = 11.84NLHH358 pKa = 6.37LAQAANCGASLVAISTGLDD377 pKa = 3.11AVPSVTGDD385 pKa = 3.54CTATAPEE392 pKa = 4.0AVLTGSLTATEE403 pKa = 4.62APSTITSGPGGLTSAIAPGPGGTQTGDD430 pKa = 2.97RR431 pKa = 11.84VASSLCLTCKK441 pKa = 10.52NSATGAAHH449 pKa = 7.44PSTTTRR455 pKa = 11.84TANEE459 pKa = 3.98GAMEE463 pKa = 4.41TGSPLAAVGAMAMAALLII481 pKa = 4.38

Molecular weight: 50.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2F7L8|A0A0G2F7L8_9PEZI Probable dipeptidyl-aminopeptidase B OS=Diaporthe ampelina OX=1214573 GN=UCDDA912_g09347 PE=3 SV=1
MM1 pKa = 7.58EE2 pKa = 4.86AQKK5 pKa = 8.88PTQQASLAFRR15 pKa = 11.84PRR17 pKa = 11.84IKK19 pKa = 9.9TSLRR23 pKa = 11.84QRR25 pKa = 11.84FTKK28 pKa = 10.57QKK30 pKa = 10.75DD31 pKa = 3.63PAPKK35 pKa = 10.22APPQDD40 pKa = 3.26QQVAQQSRR48 pKa = 11.84QQNHH52 pKa = 5.29EE53 pKa = 3.69AVATHH58 pKa = 6.19FHH60 pKa = 6.87HH61 pKa = 7.31FPDD64 pKa = 4.54LPTEE68 pKa = 3.97LRR70 pKa = 11.84LQIWAEE76 pKa = 3.48AARR79 pKa = 11.84YY80 pKa = 8.39KK81 pKa = 10.42RR82 pKa = 11.84YY83 pKa = 10.08VVLEE87 pKa = 4.26PPCNSAAACARR98 pKa = 11.84LFLMAKK104 pKa = 9.84RR105 pKa = 11.84YY106 pKa = 9.23GGPGCARR113 pKa = 11.84GRR115 pKa = 11.84YY116 pKa = 8.65RR117 pKa = 11.84PPAWTSRR124 pKa = 11.84TPPPALLAVSTEE136 pKa = 3.67ARR138 pKa = 11.84AVALGTWQRR147 pKa = 11.84AFGYY151 pKa = 10.02GVFPATVMRR160 pKa = 11.84GMHH163 pKa = 6.86RR164 pKa = 11.84LTDD167 pKa = 3.29RR168 pKa = 11.84SEE170 pKa = 4.01FMNGLKK176 pKa = 10.47SLTIEE181 pKa = 4.46IRR183 pKa = 11.84DD184 pKa = 3.65NN185 pKa = 3.48

Molecular weight: 20.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10703

0

10703

4723684

66

4584

441.3

48.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.528 ± 0.025

1.123 ± 0.008

6.105 ± 0.017

6.221 ± 0.021

3.764 ± 0.014

7.641 ± 0.024

2.234 ± 0.01

4.594 ± 0.015

4.84 ± 0.023

8.799 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.009

3.574 ± 0.012

5.576 ± 0.02

3.843 ± 0.015

5.892 ± 0.022

7.418 ± 0.021

5.76 ± 0.015

6.574 ± 0.019

1.523 ± 0.01

2.791 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski