Murid herpesvirus 1 (strain K181) (MuHV-1) (Mouse cytomegalovirus)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus; Murid betaherpesvirus 1

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8E1Q2|A8E1Q2_MUHVK M137 protein OS=Murid herpesvirus 1 (strain K181) OX=69156 GN=m137 PE=4 SV=1
MM1 pKa = 7.92AINTTEE7 pKa = 4.23CPSPSEE13 pKa = 4.15TVSAQTIVLTCAALTSVLLAAAILVVAISALVRR46 pKa = 11.84RR47 pKa = 11.84CRR49 pKa = 11.84RR50 pKa = 11.84QQTPRR55 pKa = 11.84GDD57 pKa = 3.38EE58 pKa = 3.82QLPIEE63 pKa = 4.42CSVYY67 pKa = 10.08LVQNNGVDD75 pKa = 4.75DD76 pKa = 5.38DD77 pKa = 5.51DD78 pKa = 4.77IPPGFLGRR86 pKa = 11.84PAGYY90 pKa = 10.42EE91 pKa = 3.8DD92 pKa = 4.46MSPHH96 pKa = 6.63SFPLDD101 pKa = 3.9EE102 pKa = 5.83IYY104 pKa = 11.02EE105 pKa = 4.53SIDD108 pKa = 3.11

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8E1R6|A8E1R6_MUHVK M150 protein OS=Murid herpesvirus 1 (strain K181) OX=69156 GN=m150 PE=4 SV=1
MM1 pKa = 6.95TVSFFRR7 pKa = 11.84DD8 pKa = 3.43LRR10 pKa = 11.84ILLAEE15 pKa = 4.58FTATFDD21 pKa = 3.69TVPDD25 pKa = 3.88SVVGDD30 pKa = 3.37VHH32 pKa = 6.48EE33 pKa = 5.05RR34 pKa = 11.84KK35 pKa = 10.3CPTTPTEE42 pKa = 3.82PHH44 pKa = 5.86GTHH47 pKa = 5.95HH48 pKa = 6.87QEE50 pKa = 4.18DD51 pKa = 4.14ASSGVITHH59 pKa = 6.59EE60 pKa = 3.85PSEE63 pKa = 4.27RR64 pKa = 11.84SEE66 pKa = 4.17VEE68 pKa = 4.23SLPKK72 pKa = 9.14TALYY76 pKa = 8.63TKK78 pKa = 9.71SKK80 pKa = 10.69RR81 pKa = 11.84SGCHH85 pKa = 5.34AASGDD90 pKa = 3.62ASSCQSTGRR99 pKa = 11.84SHH101 pKa = 6.06VRR103 pKa = 11.84ARR105 pKa = 11.84LARR108 pKa = 11.84RR109 pKa = 11.84SAEE112 pKa = 4.62DD113 pKa = 3.27IACADD118 pKa = 3.5LSVPRR123 pKa = 11.84RR124 pKa = 11.84SQLVQTRR131 pKa = 11.84GAIAVVGPPRR141 pKa = 11.84RR142 pKa = 11.84ALRR145 pKa = 11.84VVAPEE150 pKa = 3.99KK151 pKa = 10.83HH152 pKa = 6.7IGTQDD157 pKa = 3.54PLQHH161 pKa = 6.53RR162 pKa = 11.84KK163 pKa = 8.99RR164 pKa = 11.84RR165 pKa = 11.84YY166 pKa = 9.05DD167 pKa = 3.2RR168 pKa = 11.84HH169 pKa = 7.64DD170 pKa = 3.66GDD172 pKa = 4.08QGQLHH177 pKa = 7.19AARR180 pKa = 11.84GRR182 pKa = 11.84LPLCRR187 pKa = 11.84RR188 pKa = 11.84CRR190 pKa = 11.84SQDD193 pKa = 2.85LGLVVRR199 pKa = 11.84RR200 pKa = 11.84GRR202 pKa = 11.84QRR204 pKa = 11.84HH205 pKa = 5.13RR206 pKa = 11.84PPHH209 pKa = 6.07RR210 pKa = 11.84RR211 pKa = 11.84SNDD214 pKa = 2.84TDD216 pKa = 3.43QIRR219 pKa = 11.84GLEE222 pKa = 3.85ARR224 pKa = 11.84GRR226 pKa = 11.84EE227 pKa = 4.22IVV229 pKa = 3.19

Molecular weight: 25.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

64777

84

2147

390.2

43.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.957 ± 0.206

2.221 ± 0.113

5.871 ± 0.155

5.233 ± 0.152

3.788 ± 0.106

6.623 ± 0.228

2.175 ± 0.1

4.424 ± 0.139

3.461 ± 0.124

8.526 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.077

3.284 ± 0.106

6.056 ± 0.239

2.853 ± 0.105

8.264 ± 0.214

8.61 ± 0.245

6.959 ± 0.245

7.442 ± 0.181

1.095 ± 0.068

3.01 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski