Varroa mite associated virus 1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I8B2M4|A0A2I8B2M4_9VIRU ATP-dependent helicase Rep OS=Varroa mite associated virus 1 OX=2077300 PE=3 SV=1
MM1 pKa = 7.33SRR3 pKa = 11.84SKK5 pKa = 10.68NWQFTINNHH14 pKa = 5.27GAVDD18 pKa = 3.78PLLCQWRR25 pKa = 11.84RR26 pKa = 11.84LLEE29 pKa = 3.92QGKK32 pKa = 9.84INYY35 pKa = 7.59LVCQCEE41 pKa = 4.04RR42 pKa = 11.84GDD44 pKa = 3.51NGTPHH49 pKa = 6.16IQGMLQCKK57 pKa = 9.28NAISMQSAKK66 pKa = 10.71DD67 pKa = 3.51RR68 pKa = 11.84ASSNAHH74 pKa = 5.1VEE76 pKa = 3.97VAIRR80 pKa = 11.84PKK82 pKa = 10.15EE83 pKa = 3.74LRR85 pKa = 11.84AYY87 pKa = 10.34CMKK90 pKa = 10.58EE91 pKa = 3.75DD92 pKa = 3.79TRR94 pKa = 11.84EE95 pKa = 4.01LGPWEE100 pKa = 3.71IGEE103 pKa = 4.18FRR105 pKa = 11.84NEE107 pKa = 3.63QGKK110 pKa = 7.73RR111 pKa = 11.84TDD113 pKa = 3.9LDD115 pKa = 3.7LARR118 pKa = 11.84QAIDD122 pKa = 5.06DD123 pKa = 4.34GATDD127 pKa = 3.54AMLAKK132 pKa = 9.89EE133 pKa = 4.16HH134 pKa = 6.69FSTFVKK140 pKa = 9.59YY141 pKa = 10.46HH142 pKa = 6.68KK143 pKa = 10.21GLSAARR149 pKa = 11.84MHH151 pKa = 6.44IQRR154 pKa = 11.84PTNHH158 pKa = 6.89YY159 pKa = 9.21IPKK162 pKa = 9.98KK163 pKa = 9.25VVLFHH168 pKa = 6.56GMPGTGKK175 pKa = 8.97TYY177 pKa = 10.49FCLVTLGLDD186 pKa = 3.46PASVYY191 pKa = 11.12VKK193 pKa = 9.57DD194 pKa = 4.84TRR196 pKa = 11.84TKK198 pKa = 9.0WWDD201 pKa = 3.29GYY203 pKa = 11.07AGQKK207 pKa = 8.8VVVMDD212 pKa = 4.63EE213 pKa = 4.54FPGSLSALDD222 pKa = 3.19WKK224 pKa = 10.99LYY226 pKa = 10.37LGEE229 pKa = 4.56AMVALEE235 pKa = 4.35SKK237 pKa = 10.38GGTMYY242 pKa = 10.69SQYY245 pKa = 9.22EE246 pKa = 4.34TVYY249 pKa = 9.78LTSNYY254 pKa = 10.15AKK256 pKa = 10.76EE257 pKa = 4.15EE258 pKa = 4.11LFPNARR264 pKa = 11.84DD265 pKa = 3.4IDD267 pKa = 3.87RR268 pKa = 11.84QAVQRR273 pKa = 11.84RR274 pKa = 11.84ISEE277 pKa = 4.21SYY279 pKa = 9.63EE280 pKa = 3.44IRR282 pKa = 11.84DD283 pKa = 3.61RR284 pKa = 11.84EE285 pKa = 4.19VDD287 pKa = 4.12FLPFLEE293 pKa = 4.52NYY295 pKa = 8.58VRR297 pKa = 11.84PP298 pKa = 4.03

Molecular weight: 34.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I8B2M4|A0A2I8B2M4_9VIRU ATP-dependent helicase Rep OS=Varroa mite associated virus 1 OX=2077300 PE=3 SV=1
MM1 pKa = 7.27YY2 pKa = 10.15ARR4 pKa = 11.84KK5 pKa = 9.28YY6 pKa = 9.92SYY8 pKa = 10.34RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84PTVRR15 pKa = 11.84RR16 pKa = 11.84YY17 pKa = 8.45GSKK20 pKa = 9.82RR21 pKa = 11.84RR22 pKa = 11.84TYY24 pKa = 10.19GKK26 pKa = 10.35RR27 pKa = 11.84IAKK30 pKa = 9.15LRR32 pKa = 11.84KK33 pKa = 7.21PMRR36 pKa = 11.84RR37 pKa = 11.84AVVHH41 pKa = 4.35VMNRR45 pKa = 11.84RR46 pKa = 11.84VEE48 pKa = 4.16TKK50 pKa = 10.37YY51 pKa = 10.66FYY53 pKa = 9.79STPGPYY59 pKa = 9.91YY60 pKa = 9.51PAPQEE65 pKa = 3.85LWRR68 pKa = 11.84TDD70 pKa = 3.79LLSAIPKK77 pKa = 8.16GTLGSQRR84 pKa = 11.84IGDD87 pKa = 3.84VVNIANVQVKK97 pKa = 9.15YY98 pKa = 10.27QVTITGVTEE107 pKa = 4.19TQGTTTVTTHH117 pKa = 6.69FLNSGGGKK125 pKa = 9.41AAAAPRR131 pKa = 11.84SMLGVLTKK139 pKa = 10.35KK140 pKa = 10.51QIMSFLYY147 pKa = 8.39WQKK150 pKa = 11.45DD151 pKa = 3.61EE152 pKa = 5.76AGTTPVGVSKK162 pKa = 11.53NEE164 pKa = 3.75VFKK167 pKa = 11.1GVSPDD172 pKa = 3.36FVTDD176 pKa = 4.14VIDD179 pKa = 3.72NRR181 pKa = 11.84HH182 pKa = 4.6ITILATRR189 pKa = 11.84KK190 pKa = 9.89HH191 pKa = 5.89VVNPMNVNGAHH202 pKa = 5.94RR203 pKa = 11.84VYY205 pKa = 10.27GTIFKK210 pKa = 11.22SMGQGSNYY218 pKa = 9.93VYY220 pKa = 10.64EE221 pKa = 5.2RR222 pKa = 11.84DD223 pKa = 4.24LGDD226 pKa = 3.71GKK228 pKa = 10.9KK229 pKa = 10.2KK230 pKa = 10.73SLLFGIIVDD239 pKa = 4.32RR240 pKa = 11.84VTTPLDD246 pKa = 3.25VTVLHH251 pKa = 6.16TEE253 pKa = 4.56EE254 pKa = 4.24IRR256 pKa = 11.84FRR258 pKa = 11.84DD259 pKa = 3.43AA260 pKa = 4.6

Molecular weight: 29.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

558

260

298

279.0

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.452 ± 0.715

1.075 ± 0.72

5.197 ± 0.647

5.018 ± 1.3

3.226 ± 0.1

7.527 ± 0.626

2.509 ± 0.135

4.48 ± 0.348

6.81 ± 0.333

7.168 ± 0.937

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.047 ± 0.237

3.943 ± 0.065

4.301 ± 0.211

3.943 ± 0.58

8.065 ± 0.781

5.197 ± 0.132

7.348 ± 1.777

8.244 ± 1.692

1.434 ± 0.445

5.018 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski