Arthrobacter phage MediumFry

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; unclassified Gordonvirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KNG5|A0A345KNG5_9CAUD Minor tail protein OS=Arthrobacter phage MediumFry OX=2250415 GN=21 PE=4 SV=1
MM1 pKa = 7.53TKK3 pKa = 10.5LIWGSAGKK11 pKa = 10.11RR12 pKa = 11.84FYY14 pKa = 10.89EE15 pKa = 4.43AGTDD19 pKa = 3.41RR20 pKa = 11.84GVLYY24 pKa = 11.02LDD26 pKa = 3.68GTGVAWDD33 pKa = 3.92GLISVDD39 pKa = 3.83EE40 pKa = 4.41SPSGGEE46 pKa = 4.0SEE48 pKa = 4.28KK49 pKa = 10.9YY50 pKa = 10.44YY51 pKa = 10.81YY52 pKa = 10.77DD53 pKa = 3.61GVPYY57 pKa = 10.33ISSSSMEE64 pKa = 3.97EE65 pKa = 3.81FKK67 pKa = 11.18ASLEE71 pKa = 4.19AYY73 pKa = 6.48TWPVEE78 pKa = 4.32FSQCDD83 pKa = 3.48GSEE86 pKa = 4.31EE87 pKa = 4.1IAQGLYY93 pKa = 10.0ISQQSRR99 pKa = 11.84KK100 pKa = 9.32EE101 pKa = 3.72FGLSYY106 pKa = 9.77RR107 pKa = 11.84TLIGNDD113 pKa = 3.48LDD115 pKa = 4.21EE116 pKa = 5.67LEE118 pKa = 4.98HH119 pKa = 6.72GYY121 pKa = 10.18KK122 pKa = 9.67IHH124 pKa = 6.9IVYY127 pKa = 9.81HH128 pKa = 6.36AMAEE132 pKa = 4.25PSSRR136 pKa = 11.84TYY138 pKa = 10.62STMGDD143 pKa = 3.57DD144 pKa = 4.16TDD146 pKa = 3.65PTTFKK151 pKa = 10.55WNISTRR157 pKa = 11.84PIKK160 pKa = 10.78FEE162 pKa = 4.47DD163 pKa = 3.48PAFGIRR169 pKa = 11.84YY170 pKa = 7.63GAHH173 pKa = 6.4LVLDD177 pKa = 3.91SRR179 pKa = 11.84EE180 pKa = 4.08VYY182 pKa = 8.56PWAMAAVEE190 pKa = 4.1EE191 pKa = 4.42VLYY194 pKa = 8.87GTDD197 pKa = 2.92TTAPRR202 pKa = 11.84LPTPRR207 pKa = 11.84EE208 pKa = 3.92LLDD211 pKa = 4.35LFIDD215 pKa = 3.81NALLKK220 pKa = 9.8ITDD223 pKa = 4.11NGDD226 pKa = 3.63GTWTADD232 pKa = 3.65GPDD235 pKa = 4.76SIIQMLGSDD244 pKa = 4.54MFTIDD249 pKa = 3.76WPSVIPLDD257 pKa = 4.02EE258 pKa = 4.53DD259 pKa = 4.15TVQISSLL266 pKa = 3.6

Molecular weight: 29.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KNL1|A0A345KNL1_9CAUD NUMOD4 domain-containing protein OS=Arthrobacter phage MediumFry OX=2250415 GN=69 PE=4 SV=1
MM1 pKa = 7.64GSGLKK6 pKa = 10.13PVVKK10 pKa = 9.46PVCPVCGIAMRR21 pKa = 11.84YY22 pKa = 7.2YY23 pKa = 10.64ARR25 pKa = 11.84DD26 pKa = 3.56PNMHH30 pKa = 6.5HH31 pKa = 6.52SFWALTEE38 pKa = 4.05ASYY41 pKa = 11.07QHH43 pKa = 6.85LKK45 pKa = 10.52DD46 pKa = 4.43ANCKK50 pKa = 9.58PPEE53 pKa = 4.28PEE55 pKa = 3.9VKK57 pKa = 9.83RR58 pKa = 11.84DD59 pKa = 3.33LSEE62 pKa = 3.84VRR64 pKa = 11.84KK65 pKa = 9.67APVVIRR71 pKa = 11.84SYY73 pKa = 10.58RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84SIHH79 pKa = 6.56RR80 pKa = 11.84LPSSKK85 pKa = 9.94RR86 pKa = 11.84RR87 pKa = 11.84ALVV90 pKa = 3.05

Molecular weight: 10.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17569

41

1527

206.7

23.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.885 ± 0.384

0.495 ± 0.088

6.033 ± 0.293

6.694 ± 0.37

3.728 ± 0.196

7.656 ± 0.472

1.895 ± 0.2

6.079 ± 0.251

6.369 ± 0.391

7.804 ± 0.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.256

4.724 ± 0.208

4.075 ± 0.26

3.597 ± 0.143

5.168 ± 0.309

5.777 ± 0.216

6.233 ± 0.321

6.933 ± 0.226

1.611 ± 0.175

3.324 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski