Actinocrispum wychmicini

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinocrispum

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10734 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2S874|A0A4V2S874_9PSEU RNA polymerase sigma factor (Sigma-70 family) OS=Actinocrispum wychmicini OX=1213861 GN=EV192_102889 PE=3 SV=1
MM1 pKa = 7.4NEE3 pKa = 4.02CHH5 pKa = 7.45DD6 pKa = 4.76DD7 pKa = 3.85VCITCSDD14 pKa = 3.6TAVQVTVVRR23 pKa = 11.84LLDD26 pKa = 3.88DD27 pKa = 3.58EE28 pKa = 4.57MAVVDD33 pKa = 4.1TGSGAGSEE41 pKa = 4.53EE42 pKa = 4.45EE43 pKa = 4.14VSVALVSAKK52 pKa = 10.75VGDD55 pKa = 4.08TVLVHH60 pKa = 6.9ASEE63 pKa = 5.27AIAVVGDD70 pKa = 3.65QPP72 pKa = 4.2

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2JC86|A0A4R2JC86_9PSEU Uncharacterized protein OS=Actinocrispum wychmicini OX=1213861 GN=EV192_110309 PE=4 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84AHH5 pKa = 6.41HH6 pKa = 6.07LAVHH10 pKa = 6.93KK11 pKa = 10.24AQKK14 pKa = 8.45ATKK17 pKa = 8.82TKK19 pKa = 7.72TTIHH23 pKa = 6.38RR24 pKa = 11.84RR25 pKa = 11.84HH26 pKa = 5.78GNITRR31 pKa = 11.84KK32 pKa = 9.76LRR34 pKa = 11.84GHH36 pKa = 5.96LRR38 pKa = 11.84VRR40 pKa = 11.84GVKK43 pKa = 7.9FHH45 pKa = 7.04RR46 pKa = 11.84VRR48 pKa = 11.84HH49 pKa = 5.43PEE51 pKa = 3.8WLPGVHH57 pKa = 5.87PHH59 pKa = 5.69GVVRR63 pKa = 11.84VVAGHH68 pKa = 6.01RR69 pKa = 11.84TMSAATKK76 pKa = 10.43AKK78 pKa = 9.86IAAALKK84 pKa = 9.88GKK86 pKa = 9.22PNPHH90 pKa = 7.29PGRR93 pKa = 11.84PLSAAARR100 pKa = 11.84AKK102 pKa = 10.36ISAKK106 pKa = 10.51LKK108 pKa = 9.26GKK110 pKa = 7.31QHH112 pKa = 6.91PGHH115 pKa = 7.03PLSASARR122 pKa = 11.84AKK124 pKa = 10.33ISAKK128 pKa = 10.46LKK130 pKa = 9.19GKK132 pKa = 8.79HH133 pKa = 5.93HH134 pKa = 7.26PGHH137 pKa = 6.88PLSAATKK144 pKa = 9.36AKK146 pKa = 10.27ISAKK150 pKa = 10.29LKK152 pKa = 9.86GKK154 pKa = 8.79HH155 pKa = 5.13HH156 pKa = 7.29PGRR159 pKa = 11.84KK160 pKa = 8.37GVHH163 pKa = 5.96HH164 pKa = 7.1KK165 pKa = 10.12GHH167 pKa = 7.11PVTAATRR174 pKa = 11.84AKK176 pKa = 10.17ISAALKK182 pKa = 9.75GRR184 pKa = 11.84KK185 pKa = 8.73HH186 pKa = 5.37PHH188 pKa = 6.19RR189 pKa = 11.84PAHH192 pKa = 4.79PHH194 pKa = 5.53ARR196 pKa = 11.84VAHH199 pKa = 7.41RR200 pKa = 11.84IQQHH204 pKa = 4.63RR205 pKa = 11.84RR206 pKa = 11.84GG207 pKa = 3.66

Molecular weight: 22.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10734

0

10734

3650074

25

8028

340.0

36.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.642 ± 0.032

0.828 ± 0.008

6.273 ± 0.02

4.936 ± 0.023

2.937 ± 0.01

8.817 ± 0.027

2.298 ± 0.011

3.644 ± 0.014

2.085 ± 0.017

10.304 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.896 ± 0.01

2.183 ± 0.015

5.9 ± 0.019

3.076 ± 0.015

7.723 ± 0.025

5.244 ± 0.016

6.455 ± 0.023

9.079 ± 0.024

1.587 ± 0.01

2.093 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski