Kineobactrum salinum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Kineobactrum

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C0U7B6|A0A6C0U7B6_9GAMM Acyl-CoA_dh_1 domain-containing protein OS=Kineobactrum salinum OX=2708301 GN=G3T16_17055 PE=4 SV=1
MM1 pKa = 6.84TCTRR5 pKa = 11.84VSLNAGDD12 pKa = 3.54TWVIGSIGGAVAVSGPSSLSTTASTSSDD40 pKa = 3.41VFDD43 pKa = 5.83GISANNTAAVDD54 pKa = 3.67TTVNEE59 pKa = 4.3GTDD62 pKa = 3.36VSVSMTRR69 pKa = 11.84TGPGTLVQLDD79 pKa = 3.81EE80 pKa = 4.82FVINLNPRR88 pKa = 11.84YY89 pKa = 8.61TGGQPQNLTMTANLDD104 pKa = 3.53PAFQIQNGSPFSQNGWDD121 pKa = 3.76CTVAGQTVNCTRR133 pKa = 11.84ADD135 pKa = 3.31GGGDD139 pKa = 3.11VGGEE143 pKa = 3.74FDD145 pKa = 4.46HH146 pKa = 7.53NIGQVSIDD154 pKa = 3.22VRR156 pKa = 11.84AIAFGNNLGNTATVSSVSPDD176 pKa = 3.49DD177 pKa = 4.05AQLANNSDD185 pKa = 3.75NLNITVQEE193 pKa = 4.17PDD195 pKa = 3.78FDD197 pKa = 4.25LQALKK202 pKa = 10.7SGPTQTLRR210 pKa = 11.84VVNGEE215 pKa = 4.04SFSFNLRR222 pKa = 11.84VRR224 pKa = 11.84NNSNVAFVGEE234 pKa = 4.05LTMTDD239 pKa = 3.63SLPAGLRR246 pKa = 11.84VDD248 pKa = 4.32SYY250 pKa = 12.05SLNGWSCGPATPVNGADD267 pKa = 3.88TISCTRR273 pKa = 11.84TYY275 pKa = 11.09TEE277 pKa = 4.66GSPLAAGATTPSVLLDD293 pKa = 3.72TVATATGSHH302 pKa = 6.23SNQMCVTNTPAPGYY316 pKa = 9.48VFPADD321 pKa = 3.31SDD323 pKa = 4.06GANDD327 pKa = 4.09CDD329 pKa = 3.61NSAVEE334 pKa = 4.3GQLNADD340 pKa = 3.56SADD343 pKa = 3.22IQVYY347 pKa = 9.95KK348 pKa = 10.56SGSATVVAGEE358 pKa = 3.99LLSYY362 pKa = 10.46QLEE365 pKa = 4.36IVNAGPEE372 pKa = 4.13PAQSVTLTDD381 pKa = 3.39TFGSLFTGGPGNGFEE396 pKa = 4.36GAVVVAGNATLGSCSNAAAANVTSRR421 pKa = 11.84NLSCTFSDD429 pKa = 4.72MPVCSQGVDD438 pKa = 3.49CPVITVQVRR447 pKa = 11.84PLGSASNGTDD457 pKa = 3.21LNRR460 pKa = 11.84NNTASAFSSVTSDD473 pKa = 3.08PVYY476 pKa = 11.22GNNSDD481 pKa = 3.47NHH483 pKa = 5.62ATTVTGQADD492 pKa = 3.02IAAIKK497 pKa = 7.56TASWAEE503 pKa = 4.06DD504 pKa = 3.98PDD506 pKa = 5.23PIAAGTNLSYY516 pKa = 10.78TITVRR521 pKa = 11.84NEE523 pKa = 3.49STGASGASTVSMSDD537 pKa = 3.37TLPLDD542 pKa = 3.28VTFISASASDD552 pKa = 3.91GGSCGVTPAVGATTEE567 pKa = 4.1AGDD570 pKa = 3.73RR571 pKa = 11.84TVEE574 pKa = 4.3CTWASVQRR582 pKa = 11.84GGQRR586 pKa = 11.84TVSLVVRR593 pKa = 11.84PNRR596 pKa = 11.84GTQASTLSNTVTSATSTPEE615 pKa = 3.57VTLDD619 pKa = 3.79NNDD622 pKa = 3.55ADD624 pKa = 3.68VDD626 pKa = 4.37VPVAAPEE633 pKa = 3.91VDD635 pKa = 3.59LATTQVDD642 pKa = 3.98SPDD645 pKa = 3.66PLQIDD650 pKa = 3.66EE651 pKa = 4.68TVVYY655 pKa = 9.38TLTVRR660 pKa = 11.84NLGPSVAEE668 pKa = 3.83TVRR671 pKa = 11.84LYY673 pKa = 11.16NVLPTSGLSFLGFQYY688 pKa = 11.11VDD690 pKa = 3.42GGGITQPGLPPGVSCPVQPAVGEE713 pKa = 4.04FGKK716 pKa = 8.75TVDD719 pKa = 4.33RR720 pKa = 11.84VDD722 pKa = 3.55TALLTPSDD730 pKa = 4.16PAWLNGAWDD739 pKa = 4.22PEE741 pKa = 4.44LDD743 pKa = 3.91NADD746 pKa = 4.32IICDD750 pKa = 3.6VSALMLAGDD759 pKa = 5.0DD760 pKa = 4.65ISFQMLLDD768 pKa = 4.16AEE770 pKa = 4.4ASGVYY775 pKa = 8.26TNYY778 pKa = 10.17MIARR782 pKa = 11.84SQEE785 pKa = 3.81HH786 pKa = 7.06RR787 pKa = 11.84DD788 pKa = 3.47GFDD791 pKa = 3.69DD792 pKa = 4.37VNPANDD798 pKa = 4.56LEE800 pKa = 4.54PEE802 pKa = 4.07NTTVRR807 pKa = 11.84TRR809 pKa = 11.84ADD811 pKa = 3.25MEE813 pKa = 4.62VASKK817 pKa = 8.99TADD820 pKa = 3.64PGSVSLFEE828 pKa = 4.23PFEE831 pKa = 4.03YY832 pKa = 10.64SIVVQNNGPHH842 pKa = 6.14EE843 pKa = 4.31ALGVEE848 pKa = 4.73LEE850 pKa = 4.48DD851 pKa = 3.84SFPAGMEE858 pKa = 4.14LLGAPAVVVDD868 pKa = 3.68QGSFEE873 pKa = 4.31TTSCSGVAGDD883 pKa = 4.37TAVTCDD889 pKa = 3.99FGEE892 pKa = 4.16ISADD896 pKa = 2.98GRR898 pKa = 11.84ATVTLPVRR906 pKa = 11.84LISAAGAAVNNTATVNVTGLTLDD929 pKa = 3.99DD930 pKa = 3.91TPGNNSNDD938 pKa = 3.0GSVTVVRR945 pKa = 11.84SSIAGRR951 pKa = 11.84VYY953 pKa = 10.45HH954 pKa = 6.8DD955 pKa = 3.48QDD957 pKa = 2.79ISGDD961 pKa = 3.81YY962 pKa = 10.93DD963 pKa = 3.45VGEE966 pKa = 4.29PGISGVTVTLTGSDD980 pKa = 3.73DD981 pKa = 3.59YY982 pKa = 11.8GNTVNRR988 pKa = 11.84STTTDD993 pKa = 2.73GTGEE997 pKa = 3.87YY998 pKa = 9.84RR999 pKa = 11.84FEE1001 pKa = 4.95DD1002 pKa = 4.23LAPGVYY1008 pKa = 9.42TLTEE1012 pKa = 4.0SHH1014 pKa = 6.67PAAWVDD1020 pKa = 3.59GQEE1023 pKa = 4.51TVGSVGGTTPTTDD1036 pKa = 2.86VITNIEE1042 pKa = 3.92LAAGVDD1048 pKa = 3.54ATDD1051 pKa = 3.4YY1052 pKa = 11.28NYY1054 pKa = 11.42GEE1056 pKa = 4.24FQVGSVASISGYY1068 pKa = 10.3VYY1070 pKa = 10.43HH1071 pKa = 7.32DD1072 pKa = 3.68QNEE1075 pKa = 4.11DD1076 pKa = 3.92GIFDD1080 pKa = 3.89GDD1082 pKa = 3.9EE1083 pKa = 3.85NPIAGVVVDD1092 pKa = 4.81LAGPVPGSTTTDD1104 pKa = 2.87ANGFYY1109 pKa = 10.29SFTGLAPGSYY1119 pKa = 8.78TVTEE1123 pKa = 4.03QQPTDD1128 pKa = 3.41WLDD1131 pKa = 3.84GLDD1134 pKa = 3.67TAGTVAALVSEE1145 pKa = 4.36VDD1147 pKa = 3.63DD1148 pKa = 4.31EE1149 pKa = 5.09FVVEE1153 pKa = 5.13LLAGDD1158 pKa = 5.23EE1159 pKa = 4.07AQQWNFGEE1167 pKa = 4.29IALPDD1172 pKa = 3.65APAPVPALNRR1182 pKa = 11.84TALALLSALLLLLSAGHH1199 pKa = 6.07LRR1201 pKa = 11.84RR1202 pKa = 11.84RR1203 pKa = 11.84GLLL1206 pKa = 3.44

Molecular weight: 124.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C0U0I5|A0A6C0U0I5_9GAMM Phosphopantetheine adenylyltransferase OS=Kineobactrum salinum OX=2708301 GN=coaD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.42RR12 pKa = 11.84ARR14 pKa = 11.84AHH16 pKa = 6.29GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.35NGRR28 pKa = 11.84LVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3712

0

3712

1225903

20

1787

330.3

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.009 ± 0.052

1.063 ± 0.013

5.746 ± 0.031

6.162 ± 0.035

3.556 ± 0.027

8.139 ± 0.037

2.225 ± 0.02

4.802 ± 0.032

2.665 ± 0.032

11.053 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.019

2.934 ± 0.023

4.899 ± 0.03

4.225 ± 0.031

7.05 ± 0.041

5.942 ± 0.03

5.049 ± 0.028

7.104 ± 0.033

1.406 ± 0.017

2.708 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski